Regulation of the human endothelial cell protein C receptor gene promoter by multiple Sp1 binding sites

Blood

Volumn 101, Issue 11, 2003, Pages 4393-4401

  Rance, James B ^a   Follows, George A ^b   Cockerill, Peter N ^b   Bonifer, Constanze ^b   Lane, David A ^b   Simmonds, Rachel E ^b  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; DEOXYRIBONUCLEASE I; DIMETHYL SULFATE; PREGNANCY SPECIFIC BETA1 GLYCOPROTEIN; PROTEIN C; ACTIVATED PROTEIN C RECEPTOR; BLOOD CLOTTING FACTOR; CELL SURFACE RECEPTOR; MESSENGER RNA; TRANSCRIPTION FACTOR SP1;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CELL CULTURE; CELL NUCLEUS; CELL STRAIN HEPG2; CELL STRAIN U937; CONTROLLED STUDY; DNA FLANKING REGION; DNA FOOTPRINTING; ENDOTHELIUM CELL; FEMALE; GEL MOBILITY SHIFT ASSAY; GENE CONTROL; GENE EXPRESSION; GENE MAPPING; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; SEQUENCE HOMOLOGY; TATA BOX; TRANSLATION INITIATION; CHEMISTRY; CYTOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; UMBILICAL VEIN; VASCULAR ENDOTHELIUM;

BASE SEQUENCE; BINDING SITES; BLOOD COAGULATION FACTORS; DEOXYRIBONUCLEASE I; ENDOTHELIUM, VASCULAR; GENE EXPRESSION REGULATION; HUMANS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RECEPTORS, CELL SURFACE; RESTRICTION MAPPING; RNA, MESSENGER; SEQUENCE ALIGNMENT; SP1 TRANSCRIPTION FACTOR; UMBILICAL VEINS;

EID: 0037804781     PISSN: 00064971     EISSN: None     Source Type: Journal    
DOI: 10.1182/blood-2002-05-1570     Document Type: Article

References (38)

1. Esmon CT. Regulation of blood coagulation. Biochim Biophys Acta. 2000;1477:349-360.
2. Dahlback B. Blood coagulation. Lancet. 2000; 355:1627-1632.
3. Lane DA, Mannucci PM, Bauer KA, et al. Inherited thrombophilia: part 1. Thromb Haemost. 1996;76:651-662.
4. Fukudome K, Esmon CT. Identification, cloning and regulation of a novel endothelial cell protein C/activated protein C receptor. J Biol Chem. 1994;269:26486-26491.
5. Stearns-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex. Proc Natl Acad Sci U S A. 1996;93:10212-10216.
6. Taylor FB Jr, Peer GT, Lockhart MS, Ferrell G, Esmon CT. Endothelial cell protein C receptor plays an important role in protein C activation in vivo. Blood. 2001;97:1685-1688.
7. Simmonds RE, Lane DA. Structural and functional implications of the intron/exon organization of the human endothelial cell protein C/activated protein C receptor gene: comparison with the structure of CD1/major histocompatability complex alpha 1 and alpha 2 domains. Blood. 1998; 94:632-641.
8. Hayashi T, Nakamura H, Okada A, et al. Organisation and chromosomal localization of the human endothelial protein C receptor gene. Gene. 1999;238:367-373.
9. Liang Z, Rosen ED, Castellino FJ. Nucleotide structure and characterization of the murine gene encoding the endothelial cell protein C receptor. Thromb Haemost. 1999;81:585-588.
10. Gu J, Fukudome K, Esmon CT. Characterization and regulation of the 5′ flanking region of the murine endothelial protein C receptor gene. J Biol Chem. 2000;275:12481-12488.
11. Laszik Z, Mitro A, Taylor FB Jr, Ferrell G, Esmon CT. Human protein C receptor is present primarily on endothelium of large blood vessels. Circulation. 1997;96:3633-3640.
12. Czyz M, Cierniewski CS. Selective Sp1 and Sp3 binding is crucial for activity of the integrin alphaV promoter in cultured endothelial cells. Eur J Biochem. 1999;265:638-644.
13. Gory S, Dalmon J, Prandini M, Kortulewski T, Launoit Y, Huber P. Requirement of a GT box (Sp1 Site) and two Ets binding sites for vascular endothelial cadherin gene transcription. J Biol Chem. 1998;273:6750-6755.
14. Hata Y, Duh E, Zhang K, Robinson GS, Aiello LP. Transcription factors Sp1 and Sp3 alter vascular endothelial growth factor receptor expression through a novel recognition sequence. J Biol Chem. 1998;273:19294-19303.
15. Pan J, McEver RP. Characterization of the promoter for the human p-selectin gene. J Biol Chem. 1993;268:22600-22608.
16. Lee ME, Temizer DH, Clifford JA, Quertermous T. Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells. J Biol Chem. 1991;266:16188-16192.
17. Dube A, Akbarali Y, Sato TN, Libermann TA, Oettgen P. Role of the Ets transcription factors in the regulation of the vascular-specific Tie2 gene. Circ Res. 1999;84:1177-1185.
18. Rius C, Smith JD, Almendro N, et al. Cloning of the promoter region of human endoglin, the target gene for hereditary hemorrhagic telangiectasia type 1. Blood. 1998;92:4677-4690.
19. Kappel A, Schlaeger TM, Flamme I, Orkin SH, Risau W, Breier G. Role of SCL/Tal-1, GATA and Ets transcription factor binding sites for the regulation of Flk-1 expression during murine vascular development. Blood. 2000;96:3078-3085.
20. Resnick N, Gimbrone MA Jr. Hemodynamic forces are complex regulators of endothelial gene expression. FASEB J. 1995;9:874-882.
21. Aird WC, Edelberg JM, Weiler-Guettler H, Simmons WW, Smith TW, Rosenberg RD. Vascular bed-specific expression of an endothelial cell gene is programmed by the microenvironment. J Cell Biol. 1997;138:1117-1124.
22. Tsuneyoshi N, Fukudome K, Horiguchi S, et al. Expression and anticoagulant function of the endothelial cell protein C receptor (EPCR) in cancer cell lines. Thromb Haemost. 2001;85:356-361.
23. Cockerill PN, Bert AG, Roberts D, Vadas MA. The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Proc Natl Acad Sci U S A. 1999;96:15097-15102.
24. Cockerill PN. Identification of DNasel hypersensitive sites within nuclei. Methods Mol Biol. 2000; 130:29-46.
25. Kontaraki J, Chen HH, Riggs A, Bonifer C. Chromatin fine structure profiles for a developmentally regulated gene: reorganization of the lysozyme locus before trans-activator binding and gene expression. Genes Dev. 2000;14:2106-2122.
26. Edgell CS, McDonald CC, Graham JB. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci U S A. 1983;80:3734-3737.
27. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1989.
28. Docherty K. Gene Transcription: DNA Binding Proteins. Chichester, United Kingdom: Wiley; 1996.
29. Quandt K, FrechKaras H, Wingender E, Werner T. Matlnd and Matlnspector - new, fast and versatile tools for detection of consensus matches in nucleotide sequence data. Nucleic Acids Res. 1995;23:4878-4884.
30. Gu JM, Crawley JT, Ferrell G, et al. Disruption of the endothelial cell protein C receptor gene in mice causes placental thrombosis and early embryonic lethality. J Biol Chem. 2002;277:43335-43343.
31. Hernandez-Munain C, Roberts JL, Krangel MS. Cooperation among multiple transcription factors is required for access to minimal T-cell receptor alpha-enhancer chromatin in vivo. Mol Cell Biol. 1998;18:3223-3233.
32. Hagan G, Muller S, Beato M, Suske G. Cloning by recognition of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Res. 1992;20:5519-5525.
33. Patterson C, Wu Y, Lee M, DeVault JD, Runge MS, Haber E. Nuclear protein interactions with the human KDR/flk-1 promoter in vivo. J Biol Chem. 1997;272:8410-8416.
34. Scheffer GL, Flens MJ, Hageman S, Izquierdo MA, Shoemaker RH, Scheper RJ. Expression of the vascular endothelial cell protein C receptor in epithelial tumour cells. Eur J Cancer. 2002;38: 1535-1542.
35. Galligan L, Livingstone W, Volkov Y, et al. Characterization of protein C receptor expression in monocytes. Br J Haematol. 2001;115:408-414.
36. Schlaeger TM, Bartunkova S, Lawitts JA, et al. Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice. Proc Natl Acad Sci U S A. 1997;94: 3058-3063.
37. Kappel A, Ronicke V, Damert A, Flamme I, Risau W, Breier G. Identification of vascular endothelial growth factor (VEGF) receptor-2 (Flk-1) promoter enhancer sequences sufficient for angioblast and endothelial cell-specific transcription in transgenic mice. Blood. 1999;93:4284-4292.
38. Ades EW, Candal FJ, Swerlick RA, et al. HMEC-1: establishment of an immortalized human microvascular endothelial cell line. J Invest Dermatol. 1992;99:683-690.