Fully Automated Assessment of Inflammatory Cell Counts and Cytokine Expression in Bronchial Tissue

American Journal of Respiratory and Critical Care Medicine

Volumn 167, Issue 11, 2003, Pages 1496-1503

  Sont, Jacob K ^a   De Boer, Willem I ^b   Van Schadewijk, W Annemarie A M ^b   Grünberg, Katrien ^b   Van Krieken, J Han J M ^b   Hiemstra, Pieter S ^b   Sterk, Peter J ^b  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b NONE

Author keywords

Airway inflammation; Asthma; Biopsy (bronchial); Chronic obstructive pulmonary disease; Cytokines; Helper T cells, types 1 and 2

Indexed keywords

CYTOKINE; GAMMA INTERFERON ANTIBODY; HEMATOXYLIN; INTERLEUKIN 4; INTERLEUKIN 4 ANTIBODY; INTERLEUKIN 5; INTERLEUKIN 5 ANTIBODY; MESSENGER RNA; MONOCLONAL ANTIBODY CD3;

ADULT; ALLERGIC ASTHMA; ARTICLE; BRONCHUS; BRONCHUS BIOPSY; CELL COUNT; CYTOKINE RELEASE; DENSITOMETRY; DEVICE; FEMALE; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMAGE ANALYSIS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; INFLAMMATORY CELL; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; ALGORITHM; ASTHMA; AUTOANALYSIS; BIOPSY; BRONCHOSCOPY; FROZEN SECTION; IMAGE PROCESSING; METABOLISM; MIDDLE AGED; PATHOLOGY;

ADULT; ALGORITHMS; ASTHMA; AUTOANALYSIS; BIOPSY; BRONCHI; BRONCHOSCOPY; CELL COUNT; CYTOKINES; DENSITOMETRY; FEMALE; FROZEN SECTIONS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; MALE; MIDDLE AGED; RNA, MESSENGER;

EID: 0037794404     PISSN: 1073449X     EISSN: None     Source Type: Journal    
DOI: 10.1164/rccm.2205003     Document Type: Article

References (21)

1. de Boer WI, Hiemstra PS, Sont JK, De Heer E, Rabe KF, Van Krieken JH, Sterk PJ. Image analysis and quantification in lung tissue. Clin Exp Allergy 2000;31:504-508.
2. Trigg CJ, Manolitsas ND, Wang JH, Calderon MA, Mcaulay A, Jordan SE, Herdman MJ, Jhalli N, Duddle JM, Hamilton SA, et al. Placebo-controlled immunopathologic study of four months of inhaled corticosteroids in asthma. Am J Respir Crit Care Med 1994;150:17-22.
3. Jeffery PK. Bronchial biopsies and airway inflammation. Eur Respir J 1996;9:1583-1587.
4. Faul JL, Demers EA, Burke CM, Poulter LW. The reproducibility of repeat measures of airway inflammation in stable atopic asthma. Am J Respir Crit Care Med 1999;160:1457-1461.
5. Sullivan P, Stephens D, Ansari T, Costello J, Jeffery P. Variation in the measurements of basement membrane thickness and inflammatory cell number in bronchial biopsies. Eur Respir J 1998;12:811-815.
6. Sont JK, Willems LN, Bel EH, Van Krieken JH, Vandenbroucke JP, Sterk PJ. Clinical control and histopathologic outcome of asthma when using airway hyperresponsiveness as an additional guide to long-term treatment. AMPUL Study Group. Am J Respir Crit Care Med 1999; 159:1043-1051.
7. Grünberg K, Sharon RF, Sont JK, in't Veen JC, Van Schadewijk WA, De Klerk EP, Dick CP, Van Krieken JH, Sterk PJ. Rhinovirus-induced airway inflammation in asthma: effect of treatment with inhaled corticosteroids before and during experimental infection. Am J Respir Crit Care Med 2001;164:1816-1822.
8. 1992. Anonymous. Workshop summary and guidelines: Investigative use of bronchoscopy, lavage, and bronchial biopsies in asthma and other airways diseases. J Allergy Clin Immunol 1991;88:808-814.
9. Jarjour NN, Peters SP, Djukanovic R, Calhoun WJ. Investigative use of bronchoscopy in asthma. Am J Respir Crit Care Med 1998;157:692-697.
10. Sont JK, van Krieken JM, Evertse CE, Hooijer R, Willems LA, Sterk PJ. The relationship between the inflammatory infiltrate in bronchial biopsies and clinical severity of asthma in patients treated with inhaled steroids. Thorax 1996;51:496-502.
11. de Boer WI, Schuller AG, Vermey M, van der Kwast TH. Expression of growth factors and receptors during specific phases in regenerating urothelium after acute injury in vivo. Am J Pathol 1994;145:1199-1207.
12. de Boer WI, van Schadewijk A, Sont JK, Sharma HS, Stolk J, Hiemstra PS, Van Krieken JH. Transforming growth factor β1 and recruitment of macrophages and mast cells in airways in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 1998;158:1951-1957.
13. Grashoff WF, Sont JK, Sterk PJ, Hiemstra PS, de Boer WI, Stolk J, Han J, van Krieken JM. Chronic obstructive pulmonary disease: role of bronchiolar mast cells and macrophages. Am J Pathol 1997;151:1785-1790.
14. Ruifrok AC. Quantification of immunohistochemical staining by color translation and automated thresholding. Anal Quant Cytol Histol 1997;19: 107-113.
15. Sont JK, Willems LA, Evertse CE, Sterk PJ, van Krieken JM. Repeatability of measures of inflammatory cell number in bronchial biopsies in atopic asthma. Eur Respir J 1997;10:2602-2608.
16. Altman DG. Practical statistics for medical research. London: Chapman and Hall; 1991.
17. Berger P, Lavallee J, Rouiller R, Laurent F, Marthan R, Tunon-de-Lara JM. Assessment of bronchial inflammation using an automated cell recognition system based on colour analysis. Eur Respir J 1999;14: 1394-1402.
18. Sousa AR, Poston RN, Lane SJ, Nakhosteen JA, Lee TH. Detection of GM-CSF in asthmatic bronchial epithelium and decrease by inhaled corticosteroids. Am Rev Respir Dis 1993;147:1557-1561.
19. Sousa AR, Trigg CJ, Lane SJ, Hawksworth R, Nakhosteen JA, Poston RN, Lee TH. Effect of inhaled glucocorticoids on IL-1β and IL-1 receptor antagonist (IL-1 RA) expression in asthmatic bronchial epithelium. Thorax 1997;52:407-410.
20. Smolle J. Optimization of linear image combination for segmentation in red-green-blue images. Anal Quant Cytol Histol 1996;18:323-329.
21. Underwood RA, Gibran NS, Muffley LA, Usui ML, Olerud JE. Color subtractive-computer-assisted image analysis for quantification of cutaneous nerves in a diabetic mouse model. J Histochem Cytochem 2001;49: 1285-1291.