Optimization of immobilized metal ion affinity chromatography for single-step purification of recombinant ovine growth hormone expressed in Escherichia coli

Journal of Chromatography A

Volumn 998, Issue 1-2, 2003, Pages 93-101

  Gupta, V ^a   Eshwari, A N S ^b   Panda, A K ^b   Agarwal, G P ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

^b NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Escherichia coli; Growth hormone; Iminodiacetic acid; Immobilized metal ion affinity chromatography; Proteins

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; ESCHERICHIA COLI; GELS; IONIC STRENGTH; OPTIMIZATION; PH EFFECTS;

ION AFFINITY CHROMATOGRAPHY;

HORMONES;

COPPER ION; DIGLYCINE; ETHER DERIVATIVE; NICKEL; RECOMBINANT GROWTH HORMONE; SEPHAROSE;

ARTICLE; CELL LYSATE; CONTROLLED STUDY; ESCHERICHIA COLI; FLOW RATE; HORMONE BINDING; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; IONIC STRENGTH; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; QUANTUM YIELD;

ESCHERICHIA COLI; IMA; OVIS;

EID: 0037716468     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(03)00650-2     Document Type: Article

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