Reverse transcription/polymerase chain reaction (RT/PCR) amplification of very small numbers of transcripts: The risk in misinterpreting negative results

Leukemia

Volumn 10, Issue 7, 1996, Pages 1217-1221

  Melo, J V ^a   Yan, X H ^a   Diamond, J ^a   Lin, F ^a   Cross, N C P ^a   Goldman, J M ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

BCR ABL; Chronic myeloid leukaemia; Polymerase chain reaction; Sampling

Indexed keywords

COMPLEMENTARY DNA;

ARTICLE; CELL STRAIN HL 60; CELL STRAIN K 562; CHRONIC MYELOID LEUKEMIA; DIAGNOSTIC ERROR; GENE AMPLIFICATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0030054856     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Diamond J, Goldman JM, Melo JV. BCR-ABL, ABL-BCR, BCR and ABL genes are all expressed in individual CFU-GM colonies derived from blood of patients with chronic myeloid leukemia. Blood 1995; 85: 2171-2175.
2. Keating A. Ph positive CML cell lines. Baillières Clin Haematol 1987; 1: 1021-1029.
3. Melo JV, Gordon DE, Cross NCP, Goldman JM. The ABL-BCR fusion gene is expressed in chronic myeloid leukemia. Blood 1993; 81: 158-165.
4. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156-159.
5. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979; 18: 5294-5299.
6. Cross NCP, Melo JV, Lin F, Goldman JM. An optimised multiplex polymerase chain reaction (PCR) for detection of BCR-ABL fusion mRNAs in haematological disorders. Leukemia 1994; 8: 186-189.
7. Cross NCP, Lin F, Chase A, Bungey J, Hughes TP, Goldman JM. Competitive polymerase chain reaction to estimate the number of BCR-ABL transcripts in chronic myeloid leukemia patients after bone marrow transplantation. Blood 1993; 6: 1929-1936.
8. Ouspenkaia MV, Johnston DA, Roberts WM, Estrov Z, Zipf TF. Accurate quantitation of residual B-precursors acute lymphoblastic leukemia by limiting dilution and a PCR-based detection system: a description of the method and the principles involved. Leukemia 1995; 9: 321-328.
9. Sykes PJ, Neoh SJ, Brisco MJ, Hughes E, Condon J, Morley AA. Quantitation of targets for PCR by use of limiting dilution. Biotechniques 1992; 13: 444-449.