Current recommendations for positive controls in RT-PCR assays

Leukemia

Volumn 15, Issue 7, 2001, Pages 1033-1037

  Lion, T ^a  

^a CHILDREN'S CANCER RESEARCH INSTITUTE   (Austria)

Author keywords

ABL; Control genes; Pseudogenes; RT PCR; Actin; 2 MG

Indexed keywords

BCR ABL PROTEIN; BETA ACTIN; COMPLEMENTARY DNA; DIHYDROFOLATE REDUCTASE; DNA; FRUCTOSE BISPHOSPHATE ALDOLASE; GLYCERALDEHYDE 3 PHOSPHATE; HISTONE H3; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; RNA;

ARTICLE; CONTROL; CONTROLLED STUDY; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC SELECTION; GENETIC TRANSCRIPTION; HUMAN; LABORATORY TEST; LEUKEMIA; PRACTICE GUIDELINE; PRIORITY JOURNAL; PSEUDOGENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA STABILITY;

EID: 0034900371     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.leu.2402133     Document Type: Article

References (38)

1. Appropriate controls for RT-PCR. Debate round table
2. Appropriate controls for RT-PCR. Debate round table
3. Control genes in reverse transcriptase-polymerase chain reaction assays
4. Problematic controls for reverse transcription polymerase chain reactions (RT-PCR): An issue revisited
5. Appropriate controls for RT-PCR
6. Commentary: Round-table debate on appropriate controls for RT-PCR
7. Commentary: Round-table debate on appropriate controls for RT-PCR
8. Commentary: Round-table debate on appropriate controls for RT-PCR
9. Commentary: Round-table debate on appropriate controls for RT-PCR
10. Commentary: Round-table debate on appropriate controls for RT-PCR
11. Commentary: Round-table debate on appropriate controls for RT-PCR
12. Commentary: Round-table debate on appropriate controls for RT-PCR
13. Commentary: Round-table debate on appropriate controls for RT-PCR
14. Commentary: Round-table debate on appropriate controls for RT-PCR
15. Commentary: Round-table debate on appropriate controls for RT-PCR
16. Commentary: Round-table debate on appropriate controls for RT-PCR
17. Commentary: Round-table debate on appropriate controls for RT-PCR
18. Multiplex PCR for quality control of template RNA/cDNA in RT-PCR assays
19. Control genes for reverse transcriptase polymerase chain reaction (RT-PCR)
20. Appropriate controls for reverse transcription polymerase-chain reaction (RT-PCR)
21. Controls for reverse transcriptase-polymerase chain reaction amplification of BCR-ABL transcripts
22. Control genes for reverse-transcription polymerase-chain reaction: A comparison of beta actin and glyceraldehyde phosphate dehydrogenase
23. Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference
24. Human β-actin retropseudogenes interfere with RT-PCR
25. Removal of DNA contamination in polymerase chain reaction reagents by ultraviolet irradiation
26. Processed pseudogenes interfere with reverse transcriptase polymerase chain reaction controls
27. Quantification of residual disease in chronic myelogenous leukemia patients on interferon-alpha therapy by competitive polymerase chain reaction
28. Competitive polymerase chain reaction to estimate the number of BCR/ABL transcripts in chronic myeloid leukemia after bone marrow transplantation
29. Evaluation of RNA isolation methods and reference genes for RT-PCR analyses of rare target RNA
30. Use of two reporter dyes without interference in a single-tube rapid-cycle PCR: Alpha(1)-antitrypsin genotyping by multiplex real-time fluorescence PCR with the LightCycler
31. Prevention of product carry-over by single tube two-round (ST-2R) PCR: Application to BCR-ABL analysis in chronic myelogenous leukemia
32. Use of quantitative polymerase chain reaction to monitor residual disease in chronic myelogenous leukemia during treatment with interferon
33. Evolutionary implications of the human aldolase-A, -B, -C, and -pseudogene chromosome locations
34. Structure of two human beta-actin-related processed genes one of which is located next to a simple repetitive sequence
35. A human dihydrofolate reductase pseudogene and its relationship to the multiple forms of specific messenger RNA
36. Human glyceraldehyde-3-phosphate dehydrogenase pseudogenes: Molecular evolution and possible mechanisms of amplification
37. Characterization of an unusual human histone H3.3 pseudogene
38. The presence of a pseudogene may affect the use of HPRT as an endogenous mRNA control in RT-PCR