Detection of plant parasitic nematodes, Meloidogyne incognita and Pratylenchus coffeae by multiplex PCR using specific primers

Soil Science and Plant Nutrition

Volumn 49, Issue 2, 2003, Pages 291-295

  Saeki, Yuichi ^a   Kawano, Eiji ^a   Yamashita, Chigusa ^a   Akao, Shoichiro ^a   Nagatomo, Yoshitaka ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

Internal transcribed spacer; Meloidogyne incognita; Multiplex PCR; Plant parasitic nematode; Pratylenchus coffeae

Indexed keywords

MELOIDOGYNE; MELOIDOGYNE INCOGNITA; MELOIDOGYNE JAVANICA; NEMATODA; PRATYLENCHUS; PRATYLENCHUS COFFEAE;

EID: 0037657952     PISSN: 00380768     EISSN: 17470765     Source Type: Journal    
DOI: 10.1080/00380768.2003.10410010     Document Type: Article

References (14)

1. Chacon MR, Parkhouse RME, Robinson MP, Burrows PR, and Garate T 1991: A species-specific oligonucleotide DNA probe for the identification of Meloidogyne incognita. Parasitology, 103, 315-319
2. Goto S 1964: Studies on the control of root rot nematode disease of sweet potato caused by Pratylenchus coffeae. Bull. Miyazaki Agr. Exp. Sin., 5, 1-121, 8 pis. (in Japanese with English summary)
3. Harris TS, Sandall LJ, and Powers TO 1990: Identification of single Meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial DNA. J. Nematoi, 22, 518- 524
4. Iwahori H, Sano Z-I, and Ogawa T 2000: Distribution of main plant-parasitic nematodes in sweet potato and taro fields in Kyushu and Okinawa, Japan, 1. Survey in the central and southern parts in Kyushu island (Kumamoto, Miyazaki and Kagoshima Pref.) and development of an effective DNA analysis method for species identification. Kyushu. PL Prot. Res., 46, 112-117 (in Japanese with English summary)
5. Kondo T 1992: Studies of the southern mot-knot nematode, Meloidogyne incognita (Kofoid & WHITE) on sweet potato in the former Kaijo Experiment Station. In Senchu Kenkyu no Ayumi (Progress in Nematology), Ed. K Nakasono, p. 10-14, The Japanese Nematological Society, Tsukuba (in Japanese)
6. Mizukubo T 1995: Evidence for Pratylenchus coffeae races in differential reproduction on fifteen cultivars (Nematoda: Pratylenchidae). Jpn. J. Nematoi., 25, 85-93
7. Orui Y 1998: Identification of Japanese species of the genus Meloidogyne (nematode: Meloidogynidae) by PCR-RELP analysis. App. Entomol. Zoo/., 33, 43-51
8. Powers TO and Harris TS 1993: A polymerase chain reaction method for identification of five major Meloidogyne species. J. Nematoi, 25, 1-6
9. Powers TO, Todd TC, Burnell AM, Murray PCB, Fleming CC, Szalanski AL, Adams BA, and Harris TS 1997: The rDNA internal transcrmea spacer region as a laxononuc maiR.ci nematodes. J. Nematoi., 29, 441-450
10. Uehara T, Kushida A, and Momota Y 1999: Rapid and sensitive identification of Pratylenchus spp. using reverse dot blot hybridization. Nematology, 1, 549-555
11. Uehara T, Mizukubo T, Kushida A, and Momota Y 1998a: Identification of Pratylenchus coffeae and P. loosi using specific primers for PCR amplification of ribosomal DNA. Nemato-logica, 44, 357-368
12. Uehara T, Mizukubo T, Kushida A, and Momota Y 1998b: Identification of Pratylenchus penetrans (Cobb) by PCR using ITS-based species-specific primers. Jpn. J. Nematoi., 28, 1-7
13. Vrain TC, Wakarchuk DA, Levesque AC, and Hamiiton RI 1992: Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema arnericanum group. Fundam. Appl. Nematoi, 15, 563-573
14. Waeyenberge L, Ryss A, Moens M, Pinochet J, and Vrain TC 2000: Molecular characterisation of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology, 2, 135-142