Quantification of elastase-like activity in 13 human cancer cell lines and in an immortalized human epithelial cell line by RP-HPLC

Biological Chemistry

Volumn 384, Issue 5, 2003, Pages 817-824

  Achilles, Karin ^a   Bednarski, Patrick J ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

Cancer cells; Elastase; Enzyme activity; HPLC; Inhibitor; PMN E

Indexed keywords

4 NITROANILINE; ANTINEOPLASTIC AGENT; ELASTASE; ELASTASE INHIBITOR; KETONE DERIVATIVE; PRODRUG;

ARTICLE; CANCER CELL CULTURE; CELL COUNT; CELL GROWTH; CELL IMMORTALIZATION; CELL STRAIN HL 60; CELL STRAIN MCF 7; CELL STRAIN U937; CELL VOLUME; CONTROLLED STUDY; DRUG EFFICACY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; EPITHELIUM CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HYDROLYSIS; IN VITRO STUDY; INCUBATION TIME; LEUKEMIA CELL LINE; LUNG CANCER; MOLECULAR MODEL; PANCREAS ADENOCARCINOMA; PANCREAS CANCER; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SENSITIVITY AND SPECIFICITY;

ANILINE COMPOUNDS; CELL DIVISION; CELL LINE; CELL LINE, TUMOR; CELL SIZE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; EPITHELIAL CELLS; HUMANS; LEUKOCYTE ELASTASE; NEOPLASMS;

MINK CELL FOCUS-FORMING VIRUS;

EID: 0037532966     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2003.091     Document Type: Article

References (45)

1. Achilles, K. (2001). Coumarin derivatives as protease-sensitive prodrugs. Arch. Pharm. Pharm. Med. Chem. 334, 209-215.
2. Achilles, K. (2002). Synthesis and evaluation of new elastase substrates as tripartate prodrugs. Arch. Pharm. Pharm. Med. Chem. 335, 325-330.
3. Barrett, A.J. (1981). Leukocyte elastase. Methods Enzymol. 80, 581-588.
4. Bednarski, P.J. (1992). Relationships between the aqueous chemistry and the in vitro cytotoxic activities of mixed-amine cisplatin analogues. Biochem. Pharmacol. 43, 2609-2620.
5. Berquin, I.M. and Sloane, B.F. (1994). Cysteine proteases and tumor progression. Persp. Drug Discov. Design 2, 371-388.
6. Bervar, A., Zajc, I., Sever, N., Katunuma, N., Sloane, B.F. and Lah, T.T. (2003). Invasiveness of transformed human breast epithelial cell lines is related to cathepsin B and inhibited by cysteine proteinase inhibitors. Biol. Chem. 384, 447-455.
7. Bieth, J.G. (1986). In: Regulation of Matrix Accumulation, R.P. Meacham, ed. (New York, USA: Academic Press), pp. 217-320.
8. Bjørnland, K., Buo, L., Scott, H., Konttinen, Y., Johansen, H.T. and Aasen, A.O. (1998). Polymorphonuclear elastase in human colorectal carcinoma. Int. J. Oncol. 12, 535-540.
9. Bodnar, A.G., Ouellette, M., Frolkis, M., Holt, S.E., Chiu, C.-P., Morin, G.B., Harley, C.B., Shay, J.W., Lichtsteiner, S. and Wright, W.E. (1998). Extension of life-span by introduction of telomerase into normal human cells. Science 279, 349-352.
10. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
11. Cavarra, E., Fimiani, M., Lungarella, G., Andresassi, L., de Santi M., Mazzatenta, C. and Ciccoli, L. (2002). UVA light stimulates the production of cathepsin G and elastase-like enzymes by dermal fibroblasts: a possible contribution to the remodeling of elastotic areas in sun-damaged skin. Biol. Chem. 383, 199-206.
12. Danö, K., Andreason, PA., Gröndahl-Hansen, J., Kristensen, P., Nielsen, L.S. and Skriver, L. (1985). Plasminogen activators, tissue degradation, and cancer. Adv. Cancer Res. 44, 139-266.
13. De Noncourt,-P., Robledo, O., Alain, T, Kossakowska, A.E., Urbanski, S.-J., Potworowski, E.F. and St-Pierre, Y. (2001). Leukocyte elastase in murine and human non-Hodgkin lymphomas. J. Leukoc. Biol. 70, 585-591.
14. Duffy, M.J. (1996). Proteases as prognostic markers in cancer. Clin. Cancer Res. 2, 613-618.
15. Elsässer, H.-P., Lehr, U., Agricola, B. and Kern, H.F. (1993). Structure analysis of a highly metastatic cell line PaTu 8902 from a primary human pancreatic adenocarcinoma. Virchow's Arch. B Cell Pathol. 64, 201-207.
16. Grant, A.J., Russell, P.J. and Raghavan, D. (1989). Elastase activities of human bladder cancer cell lines derived from high grade invasive tumours. Biochem. Biophys. Res. Commun. 162, 308-315.
17. Grant, A.J., Lerro, K.A. and Wu, C.W. (1990). Cell associated elastase activities in rat mammary tumor cells. Biochem. Int. 22, 1077-1084.
18. Kao, R.T. and Stern, R. (1986). Elastases in human breast carcinoma cell lines. Cancer Res. 46, 1355-1358.
19. Keppler, D., Fondanéche, M.C., Dalet-Fumeron, V., Pagano, M. and Burtin, R (1988). Immunohistochemical and biochemical study of a cathepsin B-like proteinase in human colonic cancer. Cancer Res. 48, 6855-6862.
20. Liotta, L.A. (1986). Tumor invasion and metastasis: role of the extracellular matrix. Cancer Res. 46, 1-7.
21. Lowe, F.C. and Isaacs, J.T. (1984). Biochemical methods for predicting metastatic ability of prostatic cancer utilizing the Dunning R-3327 rat prostatic adenocarcinoma system as a model. Cancer Res. 44, 744-452.
22. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
23. Neumann, S., Gunzler, G., Hennrich, N. and Lang, H. (1984). PMN-elastase assay: enzyme immunoassay for human polymorphonuclear elastase complexed with α1-proteinase inhibitor. J. Clin. Chem. Clin. Biochem. 22, 693-697.
24. Neumann, S. and Lang, H. (1995). Entzijndung. In: Lehrbuch der Klinischen Chemie und Pathobiochemie, H. Greiling, A.M. Gressner, eds. (Stuttgart, Germany: Schattauer), pp. 1289-1293.
25. Oshima, G. and Yamada, M. (1991). Preferential localisation of elastase in cytosol of human myeloid leukemia HL-60 cells. Biol. Chem. Hoppe-Seyler 372, 947-953.
26. Otto, A.M., Faderl, F. and Schönenberger, H. (1991). Dissociation of estrogenic and cytotoxic properties of an estrogen receptor binding platinum complex in human breast cancer cell lines. Cancer Res. 51, 3217-3223.
27. Pauli, B.U., Schwartz, D.E., Thonar, E. J. and Kuettner, K.E. (1983). Tumor invasion and host extracellular matrix. Cancer Metastasis Rev. 2, 129-152.
28. Powers, J.C., Gupton, B.F., Harley, A.D., Nishino, N. and Whitley, R.J. (1977). Specificity of porcine pancreatic elastase, human leukocyte elastase and cathepsin G. Inhibition with peptide chloromethyl ketones. Biochim. Biophys. Acta 485,156-166.
29. Pulford, K.A.F., Erber, W.N., Crick, J.A., Olsson, I., Mickelm, K.J., Gatter, K.C. and Mason, D.Y. (1988). Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells. J. Clin. Pathol. 41, 853-860.
30. Pyke, C., Kristensen, R, Ralfkiaer, E., Gröndahl-Hansen, J., Erksen, J., Blasi, F. and Danö, K. (1991). Urokinase-type plasminogen activator is expressed in stromal cells and its receptor in cancer cells at invasive foci in human adenocarcinomas. Am. J. Pathol. 138, 1059-1067.
31. Rochefort, H., Capony, F. and Garcia, M. (1990). Cathepsin D in breast cancer: from molecular and cellular biology to clinical applications. Cancer Cells 2, 383-388.
32. Senior, R.M., Campbell, E.J., Landis, J.A., Cox, F.R., Kuhn, C. and Koren, H.S. (1982). Elastaseof U-937 monocytelike cells. Comparisons with elastases derived from human monocytes and neutrophils and murine macrophagelike cells. J. Clin. Invest. 69, 384-393.
33. Shimada, A. and Imamura, M. (1993) Prognostic significance of cell culture in carcinoma of the oesophagus. Br. J. Surg. 80, 605-607.
34. Stein, R.L. (1983). Catalysis by human leukocyte elastase: substrate structural dependence of rate-limiting protolytic catalysis and operation of the charge relay system. J. Am. Chem. Soc. 105, 5111-5116.
35. Stein, R.L. and Trainor, D.A. (1986). Mechanism of inactivation of human leukocyte elastase by a chloromethyl ketone: kinetic and solvent isotope effect studies. Biochemistry 25, 5414-5419.
36. Stein, R.L., Strimpler, A.M., Hori, H. and Powers, J.C. (1987). Catalysis by human leukocyte elastase: mechanistic insights into specificity requirements. Biochemistry 26, 1301-1305.
37. Thomssen, C., Schmitt, M., Goretzki, L., Oppelt, P., Pache, L., Dettmar, R, Janicke, F. and Graeff, H. (1995). Prognostic value of the cysteine proteases; cathepsin B and cathepsin L in human breast cancer. Cancer Res. 1, 741-746.
38. Tömebohm, E., Egberg, N., Sablica, H., Wallin, R., Lockner, D. and Paul, C. (1992). Elastase activity in leukaemic cells and plasma in patients with acute leukaemia. Eur. J. Haematol. 49, 98-104.
39. Turpeenniemi-Hujanen, T., Thorgeirsson, U.P., Hart, I.R., Grant, S.S. and Liotta, L.A. (1985). Expression of collagenase IV (basement membran collagenase) activity in murine tumor cell hybrids that differ in metastatic potential. J. Natl. Cancer Inst. 75,99-103.
40. Vasishta, A., Baker, P.R., Preece, P.E., Wood, R.A.B. and Cuschieri, A. (1984). Serum and tissue proteinase-like peptidase activities in women undergoing total mastectomy for breast cancer. Eur. J. Cancer Clin. Oncol. 20, 203-208.
41. Yamashita, J.-I., Ogawa, M. and Shirakusa, T. (1995a). Free-form neutrophil elastase is an independent marker predicting recurrence in primary breast cancer. J. Leukoc. Biol. 57, 375-378.
42. Yamashita, J.-I., Ogawa, M. and Sakai, K. (1995b). Prognostic significance of three novel biologic factors in a clinical trial of adjuvant therapy for node-negative breast cancer. Surgery 117, 601-608.
43. Yamashita, J.-I., Tashiro, K., Yoneda, S., Kawahara, K. and Shirakusa, T. (1996). Local increase in polymorphonuclear elastase is associated with tumor invasiveness in non-small cell lung cancer. Chest 109, 1328-1334.
44. Yamashita, J.-I., Ogawa, M., Abe, M., Hayashi, N., Kurusu, Y, Kawahara, K. and T. Shirakusa (1997). Tumor neutrophil elastase is closely associated with the direct extension of non-small cell lung cancer into the aorta. Chest 111, 885-890.
45. Zeydel, M., Nakagawa, S., Biempica, L. and Takahashi, S. (1986). Collagenase and elastase production by mouse adenocarcinoma primary cultures and cloned cells. Cancer Res. 46, 6438-6445.