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Volumn 125, Issue 25, 2003, Pages 7608-7612

Synthesis-based approach toward direct sandwich immunoassay for ciguatoxin CTX3C

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; ENZYMES; IMMUNOLOGY; SYNTHESIS (CHEMICAL);

EID: 0037532666     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja034990a     Document Type: Article
Times cited : (87)

References (67)
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  • 3
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    • For reviews on marine polycyclic ether toxins, see: (a) Yasumoto, T.; Murata, M. Chem. Rev. 1993, 93, 1897.
    • (1993) Chem. Rev. , vol.93 , pp. 1897
    • Yasumoto, T.1    Murata, M.2
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    • and ref 2
    • (c) Yasumoto, T. Chem. Rec. 2001, 1, 228 and ref 2.
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    • Hokama, Y.1
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    • Ph. D Thesis, Tohoku University
    • Nagumo, Y. Ph. D Thesis, Tohoku University, 2002.
    • (2002)
    • Nagumo, Y.1
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    • note
    • d values obtained by competitive ELISA experiments should be useful for comparison of binding selectivity of the small molecules and suitable for the issue in this work.
  • 51
    • 0038222439 scopus 로고    scopus 로고
    • note
    • We have also checked the two-step binding assay using SPR (BIAcore 2000): When CTX3C (100 nM) was applied to a 10C9-immobilized sensor chip, a very weak resonance (∼40 RU) was observed. When 100 nM of antibody 3D11 was consecutively applied to the sensor chip, a large resonance (∼900 RU) appeared. Thus, the two-step binding assay using SPR also demonstrated that the two antibodies recognize the different epitopes as shown by ELISA (Table 1).
  • 66
    • 0038222436 scopus 로고    scopus 로고
    • note
    • Samples of ciguateric fish contaminated with CTX3C are not currently available, and studies of our sandwich assay to actual biological samples will be reported in due course.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.