Effects of minimally toxic levels of carbonyl cyanide P-(trifluoromethoxy) phenylhydrazone (FCCP), elucidated through differential gene expression with biochemical and morphological correlations

Toxicological Sciences

Volumn 73, Issue 2, 2003, Pages 348-361

  Kuruvilla, Sabu ^a,b   Qualls Jr , Charles W ^b   Tyler, Ronald D ^b   Witherspoon, Sam M ^b   Benavides, Gina R ^b   Yoon, Lawrence W ^b   Dold, Karen ^b   Brown, Roger H ^b   Sangiah, Subbiah ^a   Morgan, Kevin T ^b,c  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b GLAXOSMITHKLINE   (United States)

^c Aventis Pharmaceuticals   (United States)

Author keywords

Cell cycle; FCCP; Gene expression; Molecular marker; Protein synthesis; Uncoupling agent

Indexed keywords

ADENOSINE TRIPHOSPHATE; ASPARTATE AMMONIA LIGASE; BETA ACTIN; BROXURIDINE; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; CELL PROTEIN; COMPLEMENTARY DNA; CYCLINE; CYTOSKELETON PROTEIN; HEAT SHOCK PROTEIN; METHYLENETETRAHYDROFOLATE DEHYDROGENASE; MOLECULAR MARKER; PROPIDIUM IODIDE; SERINE TRANSFER RNA LIGASE;

APOPTOSIS; ARTICLE; BIOASSAY; CELL CYCLE; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DAMAGE; DOSIMETER; ENERGY METABOLISM; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENOTOXICITY; GROWTH DISORDER; HUMAN; HUMAN CELL; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARCOMA CELL; STATISTICAL SIGNIFICANCE;

ADENOSINE TRIPHOSPHATE; CARBONYL CYANIDE P-TRIFLUOROMETHOXYPHENYLHYDRAZONE; CELL CYCLE; CELL LINE, TUMOR; DOSE-RESPONSE RELATIONSHIP, DRUG; ENERGY METABOLISM; FEMALE; GENE EXPRESSION PROFILING; GENETIC MARKERS; HUMANS; INTRACELLULAR MEMBRANES; MEMBRANE POTENTIALS; MITOCHONDRIA; MUSCLES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHABDOMYOSARCOMA; RNA, MESSENGER; RNA, NEOPLASM; TRANSCRIPTION, GENETIC; UNCOUPLING AGENTS;

EID: 0037497179     PISSN: 10966080     EISSN: None     Source Type: Journal    
DOI: 10.1093/toxsci/kfg084     Document Type: Article

References (38)

1. Ahmad, N., Adhami, V. M., Afaq, F., Feyes, D. K., and Mukhtar, H. (2001). Resveratrol causes WAF-1/p21-mediated G(1)-phase arrest of cell cycle and induction of apoptosis in human epidermoid carcinoma A431 cells. Clin. Cancer Res. 7, 1466-1473.
2. Armstrong, J. S., Hornung, B., Lecane, P. Jones, D. P., and Knox, S. J. (2001). Rotenone-induced G2/M cell cycle arrest and apoptosis in a human B lymphoma cell line PW. Biochem. Biophys. Res. Commun. 289(5), 973-978.
3. Bergstrom-Porter, B., and Shelton, E. (1979). Effect of uncouplers of oxidative phosphorylation on microtuble location and surface structure in murine mast cells. Anat. Rec. 195, 375-386.
4. Blaxter, K. (1989). Energy Metabolism in Animals and Man. Cambridge University Press, Cambridge, U.K.
5. Bowtell, D. D. L. (1999). Options available-from start to finish-for obtaining expression data by microarray. Nat. Genet. Supplement 21, 25-32.
6. Bravo, R. (1986). Synthesis of nuclear protein cyclin (PCNA) and its relationship with DNA replication. Exp. Cell Res. 163, 287-293.
7. Chen, J., Peters, R., Saba, P., Lee, P., Theodoras, A., Pagano, M., Wagner, G., and Duta, A. (1996). A 39amino acid fragment of the cell cycle regulator p21 is sufficient to bind PCNA and partially inhibit DNA replication in vivo. Nucleic Acids Res. 24, 1727-1733.
8. Clapham, J. C., Arch, J. R., Chapman, H., Haynes, A., Lister, C., Moore, G. B., Piercy, V., Carter, S. A., Lehner, I., Smith, S. A., et al. (2000). Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean. Nature 406, 415-418.
9. Cossarizza, A., Baccarani-Contri, M., Kalashnikova, G., and Franceschi, C. (1993). A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodine (JC-1). Biochem. Biophys. Res. Commun. 197, 40-45.
10. Cotran, R. S., Kumar, V., and Collins, T. (1999). Robbins Pathologic Basis of Disease. W. B. Saunders Co., Philadelphia, PA.
11. Dolbeare, F., Gratzner, H. G., Pallavicini, M. G, and Gray, J. W. (1983). Flow cytometic measurement of total DNA content and incorporated bromodeoxymidine. Proc. Natl. Acad. Sci. U.S.A. 80, 5573-5578.
12. Dynlacht, B. D., Brook, A., Dembski, M., Yensh, L., and Dyson, N. (1994). DNA binding and transactivation properties of Drosophila E2F and DP proteins. Proc. Natl. Acad. Sci. U.S.A. 91, 6359-6363.
13. El-Deiry, W. S., Tokino, T., Velculescu, V. E., Levy, D. B., Parsons, R., Trent, J. M., Lin, D., Mercer, E., Kinzler, K. W., and Vogelstein, B. (1993). Waf 1, a potential mediator of p53 tumor suppression. Cell 75, 817-825.
14. Ford, S. R., Chenault, K. H., Bunton, L. S., Ginger, H. J., McCarty, J., Hall, M. S., Pangburn, S. J., Buck, L. M., and Leach, F. R. (1996). Use of firefly luciferase for ATP measurement for ATP measurement: other nucleotides enhance turnover. J. Biolumin. Chemilumin. 11, 149-167.
15. Hool, L. C., and Arthur, P. G. (2002). Decreasing cellular hydrogen peroxide with catalase mimics the effects of hypoxia on the sensitivity of the L-type Ca2++ channel to beta-adrenergic receptor stimulation in cardiac myocytes. Circ. Res. 91, 601-609.
16. Karamohamed, S., Nordstrom, T., and Nyren, P. (1999). Real-time bioluminometric method for detection of nucleoside diphosphate kinase activity. Biotechniques 26, 728-734.
17. Kearsey, J. M., Coates, P. J., Prescott, A. R., Warbrick, E., and Hall, P. A. (1995). Oncogene 11, 1675-1683.
18. Kepler, T. B., Crossby, L., and Morgan, K. T. (2002). Normalization and analysis of DNA microarray data by self-consistency and local regression. Genome Biol. 3, 1-12.
19. Lehninger, A. L., Nelson, D. L., and Cox, M. M. (1997). Principles of Biochemistry. Worth Publishers, NY.
20. Linsinger, G., Wilhelm, S., Wagner, H., and Hacker, G. (1999). Uncouplers of oxidative phosphorylation can enhance a Fas death signal. Mol. Cel. Biol. 19, 3299-3311.
21. Lohrer, H. D. (1996). Regulation of the cell cycle following DNA damage in normal and Ataxia telangiectasia cells. Experimentia 52, 316-28.
22. Maro, B., Marty, M. C., and Bornens, M. (1982). In vivo and in vitro effects of the mitochondrial uncoupler FCCP on microtubules. EMBO J. 1, 1347-1352.
23. McAllister, R. M., Melnyk, J., Finkelstein, J. Z., Adams, E. C., and Gardner, M. B. (1969).Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24, 520-526.
24. Murray, R. K., Granner, D. K., Mayes, P. A., and Rodwell, V. W. (1996). Harper's Biochemistry. Appleton and Lange, Stamford, CT.
25. Rolfe, D. F. S., Newman, J. M., Buckingham, J. A., Clark, M. G., and Brand, M. D. (1999). Contribution of mitochondrial proton leak to respiration rate in working skeletal muscle and liver and to SMR. Am. J. Physiol. 276, C692-C699.
26. Ron, D., and Hanener, J. F. (1992). CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription. Genes Dev. 6, 439-453.
27. Sagi-Eisenberg, R., and Pechat, I. (1983). Membrane potential changes during IgE-mediated histamine release from rat basophilic leukemia cells. J. Membr. Biol.75, 97-104.
28. 6(3) or rhodamine 123, is a reliable fluorescent probe to assess DΨ changes in untact cells: Implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411, 77-82.
29. Shinohara, Y., Shidzuko, B., Shinichi, K., Seiichiro, K., Shuuji, I., and Hiroshi, T. (1998). Cationic uncouplers of oxidative phosphorylation are inducers of mitochondrial phosphorylation are inducers of mitochondrial permeability transition. FEBS Lett. 428, 89-92.
30. Skulachev, V. P. (1998). Uncoupling: new approaches to an old problem of bioenergetics. Biochim. Biophys. Acta. 1363, 100-124.
31. Smiley, S. T., Reers, M., Motola-Hartshorn, C., Lin, M., Chen, A., Smith, T. W., Steele, G., and Chen, L. B. (1991). Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. U.S.A. 88, 3671-3675.
32. Smith, M. L., Chen, I. T., Zhan, Q., Bae, I., Chen, C. Y., Glimer, T. M., Kastan, M. B., O'Connor, P. M., and Furnace, A. J. (1994). Interaction of the p53 regulated protein Gadd45 with proliferating cell nuclear antigen. (1994). Science 266, 1376-1380.
33. Starkov, A. A. (1997). "Mild" uncoupling of mitochondria. Biosci. Rep. 17, 273-279.
34. Stott, F. J., Bates, S., James, M. C., McConnell, B. B., Starborg, M., Brookes, S., Palmero, I., Ryan, K., Hara, E., Vousden, K. H., et al. (1998). The alternative product from the human CDKN2A locus, p14(ARF), participates in a regulatory feedback loop with p53 and MDM2. EMBO J. 17, 5001-5014.
35. Takekawa, M., and Saito, H. (1998). A family of stress-inducible Gadd45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAP-KKK. Cell 95, 521-530.
36. Terada, H. (1990). Uncouplers of oxidative phosphorylation. Environ. Health Perspect. 87, 213-218.
37. Wang, H., Iakova, P., Wilde, M., Welm, A., Goode, T., Roesler, W. J., and Timchenko, N. A. (2001). Mol. Cell 8, 817-828.
38. Wong, A., and Cortopassi, G. A. (2002). High-throughput measurement of mitochondrial membrane potential in a neural cell line using a fluorescence plate reader. Biochem. Biophys. Res. Commun. 298, 750-754.