Determination of α-glucan molecular weight using SEC with calcofluor detection in cereal extracts

Cereal Chemistry

Volumn 80, Issue 4, 2003, Pages 485-490

  Rimsten, Lena ^a   Stenberg, Tove ^a   Andersson, Roger ^a   Andersson, Annica ^a   Åman, Per ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINITY; CEREAL PRODUCTS; MOLECULAR WEIGHT DISTRIBUTION; SIZE EXCLUSION CHROMATOGRAPHY; SODIUM HYDROXIDE;

ALKALINES; AVERAGE MOLECULAR WEIGHT; DETECTOR RESPONSE; GLUCANS; HIGH PERFORMANCE SIZE EXCLUSION CHROMATOGRAPHY; HOT WATER; SPECIFIC BINDING; WEIGHT RANGE; XYLANASES; Β-GLUCANASE;

EXTRACTION;

AMYLASE; BETA GLUCAN; HOT WATER; SODIUM HYDROXIDE;

ARTICLE; CALIBRATION; CARBOHYDRATE ANALYSIS; CHEMICAL BINDING; FRACTIONATION; GEL PERMEATION CHROMATOGRAPHY; HIGH TEMPERATURE; MOLECULAR WEIGHT; PH; SIGNAL DETECTION;

AVENA; HORDEUM VULGARE SUBSP. VULGARE;

EID: 0037480254     PISSN: 00090352     EISSN: None     Source Type: Journal    
DOI: 10.1094/CCHEM.2003.80.4.485     Document Type: Article

References (26)

1. Åman, P., and Graham, H. 1987. Analysis of total and insoluble mixed-linked (1-3),(1-4)-β-D-glucan in barley and oats. J. Agric. Food Chem. 35:704-709.
2. Anderson, I. 1990. The effect of β-glucan molecular weight on the sensitivity of dye binding assay procedures for β-glucan estimation. J. Inst. Brew. 96:323-326.
3. Andersson, A. M. M., Merker, A., Nilsson, P., Sorensen, H., and Åman, P. 1999. Chemical composition of the potential new oil crops Barbara vulgaris, Barbara verna, and Lepidium campestre. J. Sci. Food Agric. 79:179-186.
4. Bamforth, C. W., Martin, H. L., and Wainwright, T. 1979. A role for carboxypeptidase in the solubilization of barley β-glucan. J. Inst. Brew. 85:334-338.
5. Beer, M. U., Wood, P. J., and Weisz, J. 1997. Molecular weight distribution and (1→3)(1→4)-β-glucan content of consecutive extracts of various oat and barley cultivars. Cereal Chem. 74:476-480.
6. Chen, W. L., Anderson, J. W., and Gould, M. R. 1981. Effects of oat bran, oat gum and pectin on lipid metabolism of cholesterol-fed rats. Nutr. Rep. Int. 24:1093-109.
7. Davidson, M. H., Lynn, D. D., Burns, J. H., and Bova, J. 1991. The hypocholestromic effect of β-glucan in oatmeal and oat bran. J. Am. Med. Assoc. 265:1833-1839.
8. Foldager, L., and Jørgensen, K. G. 1984. The molecular weight distribution of β-glucan in wort from malts of different barley varieties at different stages of malting. Carlsberg Res. Commun. 49:525-534.
9. Forrest, I. S., and Wainwright, T. 1977. The mode of β-glucans and pentosans in barley endosperm cell walls. J. Inst. Brew. 83:279-286.
10. Jørgensen, K. G. 1988. Quantification of high molecular weight (1→3) (1→4)-β-D-glucan using Calcofluor complex formation and flow injection analysis. I. Analytical principle and its standardization. Carlsberg Res. Commun. 53:277-285.
11. Knuckles, B. E., and Chiu, M.-C. M. 1999. β-Glucanase activity and molecular weight of β-glucans in barley after various treatments. Cereal Chem. 76:92-95.
12. Knuckles, B. E., Yokoyama, W. H., and Chiu, M.-C. M. 1997. Molecular characterization of barley β-glucans by size-exclusion chromatography with multiple-angle laser light scattering and other detectors. Cereal Chem. 74:599-604.
13. Manzanares, P., Navarro, A., Sendra, J. M., and Carbonell, J. V. 1993. Determination of the average molecular weight of barley β-glucan within the range 30-100k by the Calcfluor-FIA method. J. Cereal Sci. 17:211-223.
14. McCleary, B. V., and Glennie-Holmes, M. 1985. Enzymic quantification of (1→3)(1→4)-β-D-glucan in barley and malt. J. Inst. Brew. 91:285-295.
15. Mekis, E., Pinter, G., and Bendek, G. 1987. Modified fluorometric flow-injection-analysis (FIA) method for the determination of (1→3) (1→4)-β-D-glucan. J. Inst. Brew. 93:396-398.
16. Roubrokes, J. P., Mastromauro, D. I., Andersson, R., Christensen, B. E., and Åman, P. 2000. Molecular weight, structure and shape of oat (1→3) (1→4)-β-D-glucan fractions obtained by enzymatic degradation with lichenase. Biomacromolecules 1:584-591.
17. Sundberg, B., Pettersson, D., and Åman, P. 1995. Nutritional properties of fibre-rich barley products fed to broiler chickens. J. Sci. Food Agric. 67:469-476.
18. Suortti, T. 1993. Size-exclusion chromatographic determination of β-glucan with postcolumn reaction detection. J. Chromatogr. 632:105-110.
19. Theander, O., Åman, P., Westerlund, E., Andersson, R., and Pettersson, D. 1995. Total dietary fibre determined as neutral sugar residues, uronic acid residues, and klason lignin (The Uppsala Method): Collaborative study. J. AOAC Int. 78:1030-1044.
20. Vårum, K. M., Martinsen, A., and Smidsröd, O. 1991. Fractionation and viscometric characterization of a (1→3)(1→4)-β-D-glucan from oat, and universal calibration of a high-performance-size-exclusion chromatographic system by the use of fractionated β-glucans, alginates and pullulans. Food Hydrocoll. 5:363-374.
21. Wood, P. J. 1980. The interaction of direct dyes with water soluble substituted celluloses and cereal β-glucans. Ind. Eng. Prod. Res. Dev. 19:19-23.
22. Wood, P. J., and Fulcher, R. G. 1978. Interaction of some dyes with cereal β-glucans. Cereal Chem. 55:952-966.
23. Wood, P. J., and Weisz, J. 1984. Use of calcofluor in analysis of oat β-D-glucan. Cereal Chem. 61:73-75.
24. Wood, P. J., Weisz, J., and Blackwell, B. A. 1991a. Molecular characterization of cereal β-D-glucans. Structural analysis of oat β-D-glucan and rapid structural evaluation of β-D-glucans from different sources by high-performance liquid chromatography of oligosaccharides released by lichenase. Cereal Chem. 68:31-39.
25. Wood, P. J., Weisz, J., and Mahn, W. 1991b. Molecular characteristic of cereal β-glucans. II. Size-exclusion chromatography for comparison of molecular weight. Cereal Chem. 68:530-536.
26. 13C-nuclear magnetic resonance spectroscpoy and by rapid analysis of cellulose-like regions using high-performance anion-exchange chromatography of oligosaccharides released by lichenase. Cereal Chem. 71:301-307.