The response regulator LetA regulates the stationary-phase stress response in Legionella pneumophila and is required for efficient infection of Acanthamoeba castellanii

FEMS Microbiology Letters

Volumn 219, Issue 2, 2003, Pages 241-248

  Lynch, Damien ^a   Fieser, Nicole ^a   Glöggler, Karin ^a   Forsbach Birk, Vera ^a   Marre, Reinhard ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Amoeba; Legionella pneumophila; Regulation; Stationary phase

Indexed keywords

PROTEIN; PROTEIN DOTA; PROTEIN LETA; SIGMA FACTOR RPOS; UNCLASSIFIED DRUG;

ACANTHAMOEBA CASTELLANII; AMEBIASIS; ARTICLE; CONTROLLED STUDY; GENETIC TRANSCRIPTION; LEGIONELLA PNEUMOPHILA; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION; WILD TYPE;

ACANTHAMOEBA; ACANTHAMOEBA CASTELLANII; INSERTION SEQUENCES; LEGIONELLA; LEGIONELLA PNEUMOPHILA;

EID: 0037469609     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00050-8     Document Type: Article

References (40)

1. Rowbotham T.J. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J. Clin. Pathol. 33:1980;1179-1183.
2. Harb O.S., Gao L.Y., Abu Kwaik Y. From protozoa to mammalian cells: a new paradigm in the life cycle of intracellular bacterial pathogens. Environ. Microbiol. 2:2000;251-265.
3. Swanson M.S., Hammer B.K. Legionella pneumophila pathogenesis: a fateful journey from amoebae to macrophages. Annu. Rev. Microbiol. 54:2000;567-613.
4. Barker J., Brown M.R., Collier P.J., Farrell I., Gilbert P. Relationship between Legionella pneumophila and Acanthamoeba polyphaga: physiological status and susceptibility to chemical inactivation. Appl. Environ. Microbiol. 58:1992;2420-2425.
5. Barker J., Scaife H., Brown M.R. Intraphagocytic growth induces an antibiotic-resistant phenotype of Legionella pneumophila. Antimicrob. Agents Chemother. 39:1995;2684-2688.
6. Brieland J.K., Fantone J.C., Remick D.G., LeGendre M., McClain M., Engleberg N.C. The role of Legionella pneumophila-infected Hartmannella vermiformis as an infectious particle in a murine model of Legionnaire's disease. Infect. Immun. 65:1997;5330-5333.
7. Cirillo J.D., Cirillo S.L., Yan L., Bermudez L.E., Falkow S., Tompkins L.S. Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila. Infect. Immun. 67:1999;4427-4434.
8. Cirillo J.D., Falkow S., Tompkins L.S. Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion. Infect. Immun. 62:1994;3254-3261.
9. Byrne B., Swanson M.S. Expression of Legionella pneumophila virulence traits in response to growth conditions. Infect. Immun. 66:1998;3029-3034.
10. Hammer B.K., Swanson M.S. Co-ordination of Legionella pneumophila virulence with entry into stationary phase by ppGpp. Mol. Microbiol. 33:1999;721-731.
11. Bachman M.A., Swanson M.S. RpoS co-operates with other factors to induce Legionella pneumophila virulence in the stationary phase. Mol. Microbiol. 40:2001;1201-1214.
12. Laville J., Voisard C., Keel C., Maurhofer M., Defago G., Haas D. Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco. Proc. Natl. Acad. Sci. USA. 89:1992;1562-1566.
13. Rahme L.G., Stevens E.J., Wolfort S.F., Shao J., Tompkins R.G., Ausubel F.M. Common virulence factors for bacterial pathogenicity in plants and animals. Science. 268:1995;1899-1902.
14. Cui Y., Chatterjee A., Chatterjee A.K. Effects of the two-component system comprising GacA and GacS of Erwinia carotovora subsp. carotovora on the production of global regulatory rsmB RNA, extracellular enzymes, and harpin Ecc. Mol. Plant Microbe Interact. 14:2001;516-526.
15. Johnston C., Pegues D.A., Hueck C.J., Lee A., Miller S.I. Transcriptional activation of Salmonella typhimurium invasion genes by a member of the phosphorylated response-regulator superfamily. Mol. Microbiol. 22:1996;715-727.
16. Wong S.M., Carroll P.A., Rahme L.G., Ausubel F.M., Calderwood S.B. Modulation of expression of the ToxR regulon in Vibrio cholerae by a member of the two-component family of response regulators. Infect. Immun. 66:1998;5854-5861.
17. Pernestig A.K., Melefors O., Georgellis D. Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli. J. Biol. Chem. 276:2001;225-231.
18. Heeb S., Haas D. Regulatory roles of the GacS/GacA two-component system in plant-associated and other gram-negative bacteria. Mol. Plant Microbe Interact. 14:2001;1351-1363.
19. Ausubel, F.M. (1987) Current Protocols in Molecular Biology, Greene/Wiley-Interscience, New York.
20. Edelstein P.H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J. Clin. Microbiol. 14:1981;298-303.
21. Fettes P.S., Susa M., Hacker J., Marre R. Characterization of the Legionella pneumophila gene ligA. Int. J. Med. Microbiol. 290:2000;239-250.
22. Essig A., Heinemann M., Simnacher U., Marre R. Infection of Acanthamoeba castellanii by Chlamydia pneumoniae. Appl. Environ. Microbiol. 63:1997;1396-1399.
23. O'Connell W.A., Bangsborg J.M., Cianciotto N.P. Characterization of a Legionella micdadei mip mutant. Infect. Immun. 63:1995;2840-2845.
24. Cianciotto N.P., Fields B.S. Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages. Proc. Natl. Acad. Sci. USA. 89:1992;5188-5191.
25. Hales L.M., Shuman H.A. The Legionella pneumophila rpoS gene is required for growth within Acanthamoeba castellanii. J. Bacteriol. 181:1999;4879-4889.
26. Podbielski A., Woischnik M., Leonard B.A., Schmidt K.H. Characterization of nra, a global negative regulator gene in group A streptococci. Mol. Microbiol. 31:1999;1051-1064.
27. Goodier R.I., Ahmer B.M. SirA orthologs affect both motility and virulence. J. Bacteriol. 183:2001;2249-2258.
28. Hammer B.K., Tateda E.S., Swanson M.S. A two-component regulator induces the transmission phenotype of stationary-phase Legionella pneumophila. Mol. Microbiol. 44:2002;107-118.
29. Whistler C.A., Corbell N.A., Sarniguet A., Ream W., Loper J.E. The two-component regulators GacS and GacA influence accumulation of the stationary-phase sigma factor sigmaS and the stress response in Pseudomonas fluorescens Pf-5. J. Bacteriol. 180:1998;6635-6641.
30. Mukhopadhyay S., Audia J.P., Roy R.N., Schellhorn H.E. Transcriptional induction of the conserved alternative sigma factor RpoS in Escherichia coli is dependent on BarA, a probable two-component regulator. Mol. Microbiol. 37:2000;371-381.
31. Berger K.H., Isberg R.R. Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila. Mol. Microbiol. 7:1993;7-19.
32. Hengge-Aronis R. Survival of hunger and stress: the role of rpoS in early stationary phase gene regulation in E. coli. Cell. 72:1993;165-168.
33. Fang F.C., Libby S.J., Buchmeier N.A., Loewen P.C., Switala J., Harwood J., Guiney D.G. The alternative sigma factor katF (rpoS) regulates Salmonella virulence. Proc. Natl. Acad. Sci. USA. 89:1992;11978-11982.
34. Bandyopadhyay P., Steinman H.M. Catalase-peroxidases of Legionella pneumophila: cloning of the katA gene and studies of KatA function. J. Bacteriol. 182:2000;6679-6686.
35. Blumer C., Heeb S., Pessi G., Haas D. Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proc. Natl. Acad. Sci. USA. 96:1999;14073-14078.
36. Fettes P.S., Forsbach-Birk V., Lynch D., Marre R. Overexpresssion of a Legionella pneumophila homologue of the E. coli regulator csrA affects cell size, flagellation, and pigmentation. Int. J. Med. Microbiol. 291:2001;353-360.
37. Sadosky A.B., Wiater L.A., Shuman H.A. Identification of Legionella pneumophila genes required for growth within and killing of human macrophages. Infect. Immun. 61:1993;5361-5373.
38. Sarker M.R., Cornelis G.R. An improved version of suicide vector pKNG101 for gene replacement in gram-negative bacteria. Mol. Microbiol. 23:1997;410-411.
39. Alexeyev M.F., Shokolenko I.N., Croughan T.P. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis. Gene. 160:1995;63-67.
40. Morales V.M., Backman A., Bagdasarian M. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene. 97:1991;39-47.