Gαs is palmitoylated at the N-terminal glycine

EMBO Journal

Volumn 22, Issue 4, 2003, Pages 826-832

  Kleuss, Christiane ^a   Krause, Eberhard ^b  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

Author keywords

Adenylyl cyclase; Gly2 palmitoylation; Lipid modification; Stimulatory G protein

Indexed keywords

ADENYLATE CYCLASE; GLYCINE; CAENORHABDITIS ELEGANS PROTEIN; CYSTEINE; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; ODR 3 PROTEIN, C ELEGANS; ODR-3 PROTEIN, C ELEGANS; PALMITOYL COENZYME A;

ALPHA CHAIN; AMINO TERMINAL SEQUENCE; ARTICLE; CELL STIMULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; HYDROPHOBICITY; MASS SPECTROMETRY; PALMITOYLATION; PRIORITY JOURNAL; PROTEIN PROCESSING; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; CHEMISTRY; DRUG ANTAGONISM; HUMAN; METABOLISM; PHYSIOLOGY;

ADENYLATE CYCLASE; CAENORHABDITIS ELEGANS PROTEINS; CYSTEINE; ENZYME ACTIVATION; GLYCINE; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; HETEROTRIMERIC GTP-BINDING PROTEINS; HUMANS; MASS SPECTROMETRY; PALMITOYL COENZYME A;

EID: 0037450538     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/cdg095     Document Type: Article

References (35)

1. Berthiaume, L. and Resh, M.D. (1995) Biochemical characterization of a palmitoyl acyltransferase activity that palmitoylates myristoylated proteins. J. Biol. Chem., 270, 22399-22405.
2. Beyermann, M., Fechner, K., Furkert, J., Krause, E. and Bienert, M. (1996) A single-point slight alteration set as a tool for structure-activity relationship studies of ovine corticotropin releasing factor. J. Med. Chem., 39, 3324-3330.
3. Casey, P.J. (1994) Lipid modifications of G proteins. Curr. Opin. Cell Biol., 6, 219-225.
4. Casey, P.J. (1995) Protein lipidation in cell signaling. Science, 268, 221-225.
5. Chamoun, Z., Mann, R.K., Nellen, D., von Kessler, D.P., Bellotto, M., Beachy, P.A. and Basler, K. (2001) Skinny hedgehog, an acyltransferase required for palmitoylation and activity of the hedgehog signal. Science, 293, 2080-2084.
6. 1A receptor. J. Biol. Chem., 275, 23516-23522.
7. Chen, C.A. and Manning, D.R. (2001) Regulation of G proteins by covalent modification. Oncogene, 20, 1643-1652.
8. s protein α subunit after activation by the β-adrenergic receptor or cholera toxin. J. Biol. Chem., 268, 23769-23772.
9. Duncan, J.A. and Gilman, A.G. (1996) Autoacylation of G protein α subunits. J. Biol. Chem., 271, 23594-23600.
10. Dunphy, J.T., Greentree, W.K., Manahan, C.L. and Linder, M.E. (1996) G-protein palmitoyltransferase activity is enriched in plasma membranes. J. Biol. Chem., 271, 7154-9.
11. i to these domains requires palmitoyltransferase activity. J. Biol. Chem., 276, 43300-43304.
12. o1α regulates its activity of interaction with the plasma membrane. Biochem. J., 302, 913-920.
13. sα in Escherichia coli. Purification and properties of two forms of the protein. J. Biol. Chem., 264, 409-418.
14. Hackett, M., Guo, L., Shabanowitz, J., Hunt, D.F. and Hewlett, E.L. (1994) Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis. Science, 266, 433-435.
15. sα contains an unidentified covalent modification that increases its affinity for adenylyl cyclase. Proc. Natl Acad. Sci. USA, 94, 6116-6120.
16. iα after expression in Escherichia coli. J. Biol. Chem., 265, 8243-8251.
17. oα increases its affinity for βγ. J. Biol. Chem., 266, 4654-4659.
18. Linder, M.E., Middleton, P., Hepler, J.R., Taussig, R., Gilman, A.G. and Mumby, S.M. (1993) Lipid modifications of G proteins: α subunits are palmitoylated. Proc. Natl Acad. Sci. USA, 90, 3675-3679.
19. Lobo, S., Greentree, W.K., Linder, M.E. and Deschenes, R.J. (2002) Identification of a Ras palmitoyltransferase in Saccharomyces cerevisiae. J. Biol. Chem., 277, 41268-41273.
20. Moffett, S., Brown, D.A. and Linder, M.E. (2000) Lipid-dependent targeting of G proteins into rafts. J. Biol. Chem., 275, 2191-2198.
21. s. Eur. J. Biochem. 257, 236-241.
22. Mumby, S.M., Heukeroth, R.O., Gordon, J.I. and Gilman, A.G. (1990) G-protein α-subunit expression, myristoylation, and membrane association in COS cells. Proc. Natl Acad. Sci. USA, 87, 728-732.
23. Mumby, S.M., Kleuss, C. and Gilman, A.G. (1994) Receptor regulation of G-protein palmitoylation. Proc. Natl Acad. Sci. USA, 91, 2800-2804.
24. Peitzsch, R.M. and McLaughlin, S. (1993) Binding of acylated peptides and fatty acids to phospholipid vesicles: pertinence to myristoylated proteins. Biochemistry, 32, 10436-10443.
25. Pepinsky, R.B. (1998) Identification of a palmitic acid-modified form of human Sonic hedgehog. J. Biol. Chem., 273, 14037-14045.
26. Simons, K. and Toomre, D. (2000) Lipid rafts and signal transduction. Nat. Rev. Mol. Cell Biol., 1, 31-39.
27. sα. Science, 278, 1943-1947.
28. sα and forskolin. Science, 268, 1769-1772.
29. iα. Science, 261, 218-221.
30. z GAP and other RGS proteins by palmitoylation of G protein α subunits. Science, 278, 1132-1135.
31. 13 to lipid rafts. J. Biol. Chem., 277, 32409-32412.
32. o subunit. J. Biol. Chem., 274, 37435-37442.
33. sα. J. Biol. Chem., 268, 25001-25008.
34. Weitmann, S., Wursig, N., Navarro, J.M. and Kleuss, C. (1999) A functional chimera of mammalian guanylyl and adenylyl cyclases. Biochemistry, 38, 3409-3413.
35. Weitmann, S., Schultz, G. and Kleuss, C. (2001) Adenylyl cyclase type II domains involved in Gβγ stimulation. Biochemistry, 40, 10853-10858.