Nonenzymatic palmitoylation at Cys 3 causes extra-activation of the α- subunit of the stimulatory GTP-binding protein G(s)

European Journal of Biochemistry

Volumn 257, Issue 1, 1998, Pages 236-241

  Mollner, Stefan ^a   Ferreira, Patrik ^a   Beck, Krista ^a   Pfeuffer, Thomas ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Hydroxylaminolysis; Nonenzymatic palmitoylation; Recombinant (s); Reconstitution

Indexed keywords

ADENYLATE CYCLASE; CYSTEINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HYDROXYLAMINE; PALMITIC ACID; PROTEINASE;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DOSE RESPONSE; ENZYME ACTIVITY; LEPIDOPTERA; NONHUMAN; PALMITOYLATION; PRIORITY JOURNAL; RABBIT; TURKEY (BIRD);

ACYLATION; ANIMALS; CELL LINE; CYSTEINE; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; HUMANS; HYDROLYSIS; PALMITIC ACID; RECOMBINANT PROTEINS; TRITIUM; TURKEYS;

ANIMALIA; AVES; ESCHERICHIA COLI; LEPIDOPTERA; MELEAGRIS GALLOPAVO; ORYCTOLAGUS CUNICULUS; SPODOPTERA FRUGIPERDA;

EID: 0032190173     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2570236.x     Document Type: Article

References (32)

1. Casey, P. J. (1995) Protein lipidation in cell signaling, Science 268, 221-225.
2. Wedegaertner, P. B., Wilson, P. T. & Bourne, H. R. (1995) Lipid modifications of trimeric G proteins, J. Biol. Chem. 270, 503-506.
3. Dunphy, J. T., Greentree, W. K., Manahan, C. L. & Linder, M. E. (1996) G-protein palmitoyltransferase activity is enriched in plasma membranes, J. Biol. Chem. 271, 7154-7159.
4. Das, A. K., Dasgupta, B., Bhattacharya, R. & Basu, J. (1997) Purification and biochemical characterization of a protein-palmitoyl acyltransferase from human erythrocytes, J. Biol. Chem. 272, 11021-11025.
5. Liu, L., Dudler, T. & Gelb, M. H. (1996) Purification of a protein palmitoyl-transferase that acts on H-Ras protein and on a C-terminal N-Ras peptide, J. Biol. Chem. 271, 23269-23276.
6. Berthiaume, L. & Resh, M. D. (1995) Biochemical characterization of a palmitoyl acyltransferase activity that palmitoylates myristoylated proteins, J. Biol. Chem. 270, 22399-22405.
7. Camp, L. A., Verkruyse, L. A., Afendis, S. J., Slaughter, C. A. & Hofmann, S. L. (1994) Molecular cloning and expression of palmitoyl-protein thio-esterase, J. Biol. Chem. 269, 23212-23219.
8. O'Brien, P. J., St Jules, R. S., Reddy, T. S., Bazan, N. G. & Zatz, M. (1987) Acylation of disc membrane rhodopsin may be nonenzymatic, J. Biol. Chem. 262, 5210-5215.
9. Sudo, Y., Valenzuela, D., Beck-Sickinger, A. G., Fishman, M. C. & Strittmatter, S. M. (1992) Palmitoylation alters protein activity: blockade of Go stimulation by GAP-43, EMBO J. 11, 2095-2102.
10. Duncan, J. A. & Gilman, A. G. (1996) Autoacylation of G protein alpha subunits, J. Biol. Chem. 271, 23594-23600.
11. Magee, A. I., Koyama, A. H., Malfer, C., Wen, D. & Schlesinger, M. J. (1984) Release of fatty acids from virus glycoproteins by hydroxylamine, Biochim. Biophys. Acta 798, 156-166.
12. Taylor, D. C., Weber, N., Hogge, L. R. & Underhill, E. W. (1990) A simple enzymatic method for the preparation of radiolabeled erucoyl-CoA and other long-chain fatty acyl-CoAs and their characterization by mass spectrometry, Anal. Biochem. 184, 311-316.
13. Graziano, M. P., Freissmuth, M. & Gilman, A. G. (1991) Purification of recombinant Gs alpha, Methods Enzymol. 195, 192-202.
14. Palm, D., Münch, G., Malek, D., Dees, C. & Hekman, M. (1990) Identification of a Gs-protein coupling domain to the beta-adrenoceptor using site-specific synthetic peptides. Carboxyl terminus of Gs alpha is involved in coupling to beta-adrenoceptors, FEBS Lett. 261, 294-298.
15. Asano, T., Morishita, R. & Kato, K. (1991) Immuno-affinity purification and characterization of the alpha subunits of Go type G proteins from various bovine tissues, J. Biochem. (Tokyo) 110, 571-574.
16. Roof, D. J., Applebury, M. L. & Sternweis, P. C. (1985) Relationships within the family of GTP-binding proteins isolated from bovine central nervous system, J. Biol. Chem. 260, 16242-16249.
17. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the folin phenol reagent, J. Biol. Chem. 193, 265-275.
18. Mollner, S., Beck, K. & Pfeuffer, T. (1995) Acylation of adenylyl cyclase catalyst is important for enzymic activity, FEBS Lett. 371, 241-244.
19. Wallach, J., Droste, M., Kluxen, F. W., Pfeuffer, T. & Frank, R. (1994) Molecular cloning and expression of a novel type V adenylyl cyclase from rabbit myocardium, FEBS Lett. 338, 257-263.
20. Taussig, R., Tang, W.-J. & Gilman, A. G. (1994) Expression and purification of recombinant adenylyl cyclase in Sf9 cells, Methods Enzymol. 238, 95-108.
21. 131I-labelled human growth hormone of high specific radioactivity. Biochem. J. 89, 114-123.
22. Puchwein, G., Pfeuffer, T. & Helmreich, E. J. M. (1974) Uncoupling of catecholamine activation of pigeon erythrocyte membrane adenylate cyclase by filipin, J. Biol. Chem. 249, 3232-3240.
23. Pfeuffer, T. & Metzger, H. (1982) 7-O-Hemisuccinyl-deacetyl forskolin-Sepharose: a novel affinity support for purification of adenylate cyclase, FEBS Lett. 146, 369-375.
24. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 277, 680-685.
25. Northup, J. K., Sternweis, P. C., Smigel, M. D., Schleifer, L. S., Ross, E. M. & Gilman, A. G. (1980) Purification of the regulatory component of adenylate cyclase, Proc. Natl Acad. Sci. USA 77, 6516-6520.
26. Mollner, S., Deppisch, H. & Pfeuffer, T. (1992) The alpha subunit of the stimulatory guanine-nucleotide-binding regulatory protein forms high-molecular-mass aggregates, concomitant with iloprost-induced desensitization of human platelet adenylyl cyclase, Eur. J. Biochem. 210, 539-544.
27. Sα, Mol. Pharmacol. 52, 993-999.
28. Premont, R. T., Chen, J., Ma, H. W., Ponnapalli, M. & Iyengar, R. (1992) Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases, Proc. Natl Acad. Sci. USA 89, 9809-9813.
29. 3H]palmitic acid and mutation of cysteine-3 prevents this modification, Biochemistry 32, 8057-8061.
30. Linder, M. E., Middleton, P., Hepler, J. R., Taussig, R., Gilman, A. G., Mumby, S. M. (1993) Lipid modifications of G proteins: alpha subunits are palmitoylated, Proc. Natl Acad. Sci. USA 90, 3675-3679.
31. Kleuss, C. & Gilman, A. G. (1997) Gs alpha contains an unidentified covalent modification that increases its affinity for adenylyl cyclase, Proc. Natl Acad. Sci. USA 94, 6116-6120.
32. Iiri, T., Backlund, P. S., Jones, T. L., Wedegaertner, P. B. & Bourne, H. R. (1996) Reciprocal regulation of Gs alpha by palmitate and the beta, gamma subunit, Proc. Natl Acad. Sci. USA 93, 14592-14597.