A photochemical study of cells loaded with 2′,7′-dichlorofluorescin: Implications for the detection of reactive oxygen species generated during UVA irradiation

Free Radical Biology and Medicine

Volumn 34, Issue 8, 2003, Pages 1029-1034

  Chignell, Colin F ^a,b   Sik, Robert H ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

^b Neuropharmacology Section   (United States)

Author keywords

Free radicals; HaCaT keratinocytes; Reactive oxygen species; UVA

Indexed keywords

DICHLOROFLUORESCEIN; FLUORESCEIN; HYDROGEN PEROXIDE; JUGLONE; LACTATE DEHYDROGENASE; PEROXIDASE; REACTIVE OXYGEN METABOLITE; SUPEROXIDE; UNCLASSIFIED DRUG;

ARTICLE; CELL; CELL FREE SYSTEM; CELL VIABILITY; CONCENTRATION RESPONSE; DARKNESS; DOSE RESPONSE; ENZYME RELEASE; FLUORESCENCE; HORSERADISH; IRRADIATION; KERATINOCYTE; LIGHT ABSORPTION; MEASUREMENT; OXIDATION; OXIDATION REDUCTION REACTION; PHOTOCHEMISTRY; PRIORITY JOURNAL; RADIATION EXPOSURE; ULTRAVIOLET A RADIATION;

ARMORACIA RUSTICANA;

EID: 0037446363     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(03)00022-4     Document Type: Article

References (27)

1. 2 in cultured endothelial cells. Arch. Biochem. Biophys. 302:1993;348-355.
2. Hempel S.L., Buettner G.R., O'Malley Y.Q., Wessels D.A., Flaherty D.M. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123 . Free Radic. Biol. Med. 27:1999;146-159.
3. Tyrrell R.M. Role for singlet oxygen in biological effects of ultraviolet A radiation. Methods Enzymol. 319:2000;290-296.
4. Morliere P., Santus R. Pro-oxidant role of superoxide dismutase in ultraviolet A-induced lipid peroxidation in cultured normal human skin fibroblasts. Eur. J. Biochem. 256:1998;184-189.
5. Ryter S.W., Tyrrell R.M. Singlet molecular oxygen a possible effector of eukaryotic gene expression . Free Radic. Biol. Med. 24:1998;1520-1534.
6. Morita A., Werfel T., Stege H., Ahrens C., Karmann K., Grewe M., Grether-Beck S., Ruzicka T., Kapp A., Klotz L.O., Sies H., Krutmann J. Evidence that singlet oxygen-induced human T helper cell apoptosis is the basic mechanism of ultraviolet A radiation phototherapy. J. Exp. Med. 186:1997;1763-1768.
7. Briviba K., Klotz L.O., Sies H. Toxic and signaling effects of photochemically or chemically generated singlet oxygen in biological systems. Biol. Chem. 378:1997;1259-1265.
8. Wlaschek M., Wenk J., Brenneisen P., Briviba K., Schwarz A., Sies H., Scharffetter-Kochanek K. Singlet oxygen is an early intermediate in cytokine-dependent ultraviolet A induction of interstitial collagenase in human dermal fibroblasts in vitro. FEBS Lett. 413:1997;239-242.
9. Klotz L.O., Briviba K., Sies H. Singlet oxygen mediates the activation of JNK by UVA radiation in human skin fibroblasts. FEBS Lett. 408:1997;289-291.
10. Basumodak S., Tyrrell R.M. Singlet oxygen a primary effector in the ultraviolet A/near-visible light induction of the human heme oxygenase gene . Cancer Res. 53:1993;4505-4510.
11. Tyrrell R.M., Pidoux M. Singlet oxygen involvement in the inactivation of cultured human fibroblasts by UVA (334 nm, 365 nm) and near-visible (405 nm) radiations. Photochem. Photobiol. 49:1989;407-412.
12. Cunningham M.L., Johnson J.S., Giovanazzi S.M., Peak M.J. Photosensitized production of superoxide anion by monochromatic (290-405 nm) ultraviolet irradiation of NADH and NADPH coenzymes. Photochem. Photobiol. 42:1985;125-128.
13. Berneburg M., Grether-Beck S., Kurten V., Ruzicka T., Briviba K., Sies H., Krutmann J. Singlet oxygen mediates the UVA-induced generation of the photoaging-associated mitochondrial common deletion. J. Biol. Chem. 274:1999;15345-15349.
14. Wlaschek M., Briviba K., Stricklin G.P., Sies H., Scharffetter-Kochanek K. Singlet oxygen may mediate the ultraviolet A-induced synthesis of interstitial collagenase. J. Invest. Dermatol. 104:1995;194-198.
15. Wlaschek M., Briviba K., Sies H., Scharffetter-Kochanek K. Singlet oxygen mediates the UV-induced synthesis of interstitial collagenase. J. Invest. Dermatol. 103:1994;412.
16. Scharffetter-Kochanek K., Wlaschek M., Briviba K., Sies H. Singlet oxygen induces collagenase expression in human skin fibroblasts. FEBS Lett. 331:1993;304-306.
17. Husain J., Juurlink B.H. Oligodendroglial precursor cell susceptibility to hypoxia is related to poor ability to cope with reactive oxygen species. Brain Res. 698:1995;86-94.
18. Petersen A.B., Gniadecki R., Vicanova J., Thorn T., Wulf H.C. Hydrogen peroxide is responsible for UVA-induced DNA damage measured by alkaline comet assay in HaCaT keratinocytes. J. Photochem. Photobiol. B. 59:2000;123-131.
19. Peak M.J., Peak J.G. Hydroxyl radical quenching agents protect against DNA breakage caused by both 365 nm UVA and by γ-radiation. Photochem. Photobiol. 51:1990;649-652.
20. Tobi S.E., Paul N., McMillan T.J. Glutathione modulates the level of free radicals produced in UVA-irradiated cells. J. Photochem. Photobiol. B. 57:2000;102-112.
21. Gniadecki R., Christoffersen N., Wulf H.C. Cholesterol-rich plasma membrane domains (lipid rafts) in keratinocytes importance in the baseline and UVA-induced generation of reactive oxygen species . J. Invest. Dermatol. 118:2002;582-588.
22. Marchesi E., Rota C., Fann Y.C., Chignell C.F., Mason R.P. Photoreduction of the fluorescent dye 2′-7′-dichlorofluorescein a spin trapping and direct electron spin resonance study with implications for oxidative stress measurements . Free Radic. Biol. Med. 26:1999;148-161.
23. Fusenig N.E., Breitkreutz D., Dzarlieva R.T., Boukamp P., Bohnert A., Tilgen W. Growth and differentiation characteristics of transformed keratinocytes from mouse and human skin in vitro and in vivo. J. Invest. Dermatol. 81:1983;S168-S175.
24. Noda Y., Kawazoe Y., Hakura A. Cytotoxicity of naphthoquinones toward cultured resting murine leukemia L1210 cells in the presence of glutathione, diethyl maleate, or iodoacetamide. Biol. Pharm. Bull. 20:1997;1250-1256.
25. Ollinger K., Brunmark A. Effect of hydroxy substituent position on 1,4-naphthoquinone toxicity to rat hepatocytes. J. Biol. Chem. 266:1991;21496-21503.
26. Buffinton G.D., Ollinger K., Brunmark A., Cadenas E. DT-diaphorase-catalyzed reduction of 1,4-naphthoquinone derivatives and glutathionyl-quinone conjugates. Biochem. J. 257:1989;561-571.
27. Bilski P., Belanger A.G., Chignell C.F. Photosensitization of 2′,7′-dichlorofluorescin singlet oxygen does not contribute to the formation of fluorescent oxidation product 2′,7′-dichlorofluorescein . Free Radic. Biol. Med. 33:2002;938-946.