Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope

Biophysical Journal

Volumn 71, Issue 6, 1996, Pages 3242-3250

  Akashi, Ken Ichirou ^a   Miyata, Hidetake ^a   Itoh, Hiroyasu ^b   Kinosita Jr , Kazuhiko ^a  

^a KEIO UNIVERSITY   (Japan)

^b CENTRAL RESEARCH LABORATORY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM CHLORIDE; CARDIOLIPIN; LIPID; LIPOSOME; PHOSPHATIDIC ACID; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLGLYCEROL; PHOSPHATIDYLSERINE; POTASSIUM CHLORIDE; POTASSIUM ION; SODIUM CHLORIDE;

ARTICLE; FLUORESCENCE; FLUOROMETRY; HYDRATION; IONIC STRENGTH; LIPID BILAYER; MICROSCOPY; PHASE CONTRAST MICROSCOPY; POTASSIUM TRANSPORT; TECHNIQUE; YOUNG MODULUS;

EID: 0029752766     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79517-6     Document Type: Article

References (57)

1. Büsehl, R., H. Ringsdorf, and U. Zimmermann. 1982. Electric field-induced fusion of large liposomes from natural and polymerizable lipids. FEBS Lett. 150:38-42.
2. Cohen, F. S., M. H. Akabas, J. Zimmerberg, and A. Finkelstein. 1984. Parameters affecting the fusion of unilamellar phospholipid vesicles with planar bilayer membranes. J. Cell Biol. 98:1054-1062.
3. Deamer, D., and A. D. Bangham. 1976. Large volume liposomes by an ether vaporization method. Biochim. Biophys. Acta. 443:629-634.
4. Elbaum, M., D. K. Fygenson, and A. Libchaber. 1996. Buckling microtubles in vesicles. Phys. Rev. Lett. 76:4078-4081.
5. Engelhardt, H., H. P. Duwe, and E. Sackmann. 1985. Bilayer bending elasticity measured by Fourier analysis of thermally excited surface undulations of flaccid vesicles. J. Phys. Lett. 46:L-395-L-400.
6. Evans, E., and D. Needham. 1987. Physical properties of surfactant bilayer membranes: thermal transitions, elasticity, rigidity, cohesion, and col-loidal interactions. J. Phys. Chem. 91:4219-4228.
7. Evans, E., and W. Rawicz. 1990. Entropy-driven tension and bending elasticity in condensed-fluid membranes. Phys. Rev. Lett. 64: 2094-2097.
8. Evans, E., K. Ritchie, and R. Merkel. 1995. Sensitive force technique to probe molecular adhesion and structural linkages at biological interfaces. Biophys. J. 68:2580-2587.
9. Farge, E., and P. F. Devaux. 1992. Shape changes of giant liposomes induced by an asymmetric transmembrane distribution of phospholipids. Biophys. J. 61:347-357.
10. Fraley, R., S. Subramani, P. Berg, and D. Papahadjopoulos. 1980. Introduction of liposome-encapsulated SV40 DNA into cells. J. Biol. Chem. 255:10,431-10,435.
11. Gelles, J., B. J. Schnapp, and M. P. Sheetz. 1988. Tracking kinesin-driven movements with nanometre-scale precision. Nature (London). 331: 450-453.
12. Harbich, W., and W. Helfrich. 1979. Alignment and opening of giant lecithin vesicles by electric fields. Z. Naturforsch. 34a:1063-1065.
13. Helfrich, W., and R. M. Servuss. 1984. Undulations, steric interaction and cohesion of fluid membranes. Nuovo Cimento. D3:137-151.
14. Hibino, M., M. Shigemori, H. Itoh, K. Nagayama, and K. Kinosita, Jr. 1991. Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential. Biophys. J. 59: 209-220.
15. Higashi, K., S. Suzuki, H. Fujii, and Y. Kirino. 1987. Preparation and some properties of giant liposomes and proteoliposomes. J. Biochem. 101: 433-440.
16. Hoekstra, D., T. de Boer, K. Klappe, and J. Wilshut. 1984. Fluorescence method for measuring the kinetics of fusion between biological membranes. Biochemistry. 23:5675-5681.
17. Hotani, H. 1984. Transformation pathways of liposomes. J. Mol. Biol. 178:113-120.
18. Hotani, H., and H. Miyamoto. 1990. Dynamic features of microtubles as visualized by dark-field microscopy. Adv. Biophys. 26:135-156.
19. Hub, H. H., U. Zimmermann, and H. Ringsdorf. 1982. Preparation of large unilamellar vesicles. FEBS Lett. 140:254-256.
20. Inoué, S., and R. Oldenbourg. 1995. Microscope. In Handbook of Optics, 2nd ed., Vol. II. M. Bass, editor. McGraw-Hill, New York. Chap. 17.
21. Itoh, H., M. Hibino, M. Shigemori, M. Koishi, A. Takahashi, T. Hayakawa, and K. Kinosita, Jr. 1990. Multi-shot pulsed laser fluorescence microscope system. Proc. SPIE. 1204:49-53.
22. +) antiporter. J. Biol. Chem. 265:10,522-10,526.
23. Käs, J., and E. Sackmann. 1991. Shape transitions and shape stability of giant phospholipid vesicles in pure water induced by area-to-volume changes. Biophys. J. 60:825-844.
24. Ketis, N. V., J. Girdlestone, and C. W. M. Grant. 1980. Positive cooperativity in a (dissected) lectin-membrane glycoprotein binding event. Proc. Natl. Acad. Sci. USA. 77:3788-3790.
25. Kim, S., and G. M. Martin. 1981. Preparation of cell-size unilamellar liposomes with high captured volume and defined size distribution. Biochim. Biophys. Acta. 646:1-9.
26. Kinosita, K., Jr., M. Hibino, H. Itoh, M. Shigemori, K. Hirano, Y. Kirino, and T. Hayakawa. 1992. Events of membrane electroporation visualized on a time scale from microsecond to seconds. In Guide to Electroporation and Electrofusion. D. C. Chang, B. M. Chassy, J. A. Saunders, and A. E. Sowers, editors. Academic Press, San Diego, CA. 29-46.
27. Kinosita, K., Jr., H. Itoh, S. Ishiwata, K. Hirano, T. Nishizaka, and T. Hayakawa. 1991. Dual-view microscopy with a single camera: real-time imaging of molecular orientations and calcium. J. Cell Biol. 115:67-73.
28. Kwok, R., and E. Evans. 1981. Thermoelasticity of large lecithin bilayer vesicles. Biophys. J. 35:637-652.
29. Lipowsky, R. 1991. The conformation of membranes. Nature (London). 349:475-481.
30. Longo, M. L., A. Waring, and J. A. N. Zasadzinski. 1992. Lipid bilayer surface association of lung surfactant protein SP-B, amphipathic segment detected by flow immunofluorescence. Biophys. J. 63:760-773.
31. MacDonald, R. I. 1990. Characteristics of self-quenching of the fluorescence of lipid-conjugated rhodamine in membranes. J. Biol Chem. 265:13,533-13,539.
32. Mathivet, L., S. Cribier, and P. F. Devaux. 1996. Shape change and physical properties of giant phospholipid vesicles prepared in the presence of an ac electric field. Biophys. J. 70:1112-1121.
33. Miyata, H., and H. Hotani. 1992. Morphological changes in liposomes caused by polymerization of encapsulated actin and spontaneous formation of actin bundles. Proc. Natl. Acad. Sci. USA. 89:11,547-11,551.
34. Miyata, H., and K. Kinosita, Jr. 1994. Transformation of actinencapsulating liposomes induced by cytochalasin D. Biophys. J. 67: 922-928.
35. Mueller, P., T. F. Chien, and B. Rudy. 1983. Formation and properties of cell-size lipid bilayer vesicles. Biophys. J. 44:375-381.
36. Needham, D., and E. Evans. 1988. Structure and mechanical properties of giant lipid (DMPC) vesicle bilayers from 20°C below to 10°C above the liquid crystal-crystalline phase transition at 24°C. Biochemistry. 27: 8261-8269.
37. Needham, D., and R. M. Hochmuth. 1989. Electro-mechanical permeabilization of lipid vesicles. Biophys. J. 55:1001-1009.
38. New, R. R. C. 1990. Liposomes. Oxford University Press, Oxford.
39. Noppl-Simson, D. A., and D. Needham. 1996. Avidin-biotin interactions at vesicle surfaces: adsorption and binding, cross-bridge formation, and lateral interactions. Biophys. J. 70:1391-1401.
40. Oku, N., and R. C. MacDonald. 1983a. Differential effects of alkali metal chlorides on formation of giant liposomes by freezing and thawing and dialysis. Biochemistry. 22:855-863.
41. Oku, N., and R. C. MacDonald. 1983b. Formation of giant liposomes from lipids in chaotropic ion solutions. Biochim. Biophys. Acta. 734:54-61.
42. Oku, N., J. F. Scheerer, and R. C. MacDonald. 1982. Preparation of giant liposomes. Biochim. Biophys. Acta. 692:384-388.
43. Opsahl, L. R., and W. W. Webb. 1994. Transduction of membrane tension by the ion channel alamethicin. Biophys. J. 66:71-74.
44. Rand, R. P. 1981. Interacting phospholipid bilayers: measured forces and induced structural changes. Ann. Rev. Biophys. Bioeng. 10:277-314.
45. Reeves, J. P., and R. M. Dowben. 1969. Formation and properties of thin-walled phospholipid vesicles. J. Cell. Physiol. 73:49-60.
46. Schneider, M. B., J. T. Jenkins, and W. W. Webb. 1984. Thermal fluctuations of large quasi-spherical bimoleculer phospholipid vesicles. J. Phys. (Paris). 45:1457-1472.
47. Servuss, R. M., and E. Boroske. 1979. Lamellarity of artificial phospholipid-membranes determined by photometric phase-contrast microscopy. Phys. Lett. 69A:468-470.
48. Servuss, R. M., W. Harbich, and W. Helfrich. 1976. Measurement of the curvature-elastic modulus of egg lecithin bilayers. Biochim. Biophys. Acta. 436:900-903.
49. Servuss, R. M., and W. Helfrich. 1989. Mutual adhesion of lecithin membranes at ultralow tensions. J. Phys. (Paris). 50:809-827.
50. Sokabe, M., F. Sachs, and Z. Jing. 1991. Quantitative video microscopy of patch clamped membranes stress, strain, capacitance, and stretch channel activation. Biophys. J. 59:722-728.
51. Soltesz, S. A, and D. A. Hammer. 1995. Micropipette manipulation technique for the monitoring of pH-dependent membrane lysis as induced by the fusion peptide of influenza virus. Biophys. J. 68:315-325.
52. Stoicheva, N. G., and S. W. Hui. 1994. Electrofusion of cell-size liposomes. Biochim. Biophys. Acta. 1195:31-38.
53. Szoka, F., Jr., and D. Papahadjopoulos. 1978. Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc. Natl Acad. Sci. USA. 75:4194-4198.
54. Tank, D. W., C. Miller, and W. W. Webb. 1982. Isolated-patch recording from liposomes containing functionally reconstituted chloride channels from Torpedo electroplax. Proc. Natl. Acad. Sci. USA. 79:7749-7753.
55. Teissié, J., M. P. Rols, and C. Blangero. 1989. Electrofusion of mammalian cells and giant unilamellar vesicles. In Electroporation and Electrofusion in Cell Biology. E. Neumann, A. E. Sowers, and C. A. Jordan, editors. Plenum Publishing Company, New York. 203-214.
56. Venema, K., R. Gibrat, J.-P. Grouzis, and C. Grignon. 1993. Quantitative measurement of cationic fluxes, selectivity and membrane potential using liposomes multilabelled with fluorescent probes. Biochim. Biophys. Acta. 1146:87-96.
57. Winterhalter, M., and W. Helfrich. 1992. Bending elasticity of electrically charged bilayers: coupled monolayers, neutral surfaces, and balancing stresses. J. Phys. Chem. 96:327-330.