Ferricytochrome c encapsulated in silica hydrogels: Correlation between active site dynamics and solvent structure

Biophysical Chemistry

Volumn 103, Issue 1, 2003, Pages 67-75

  Santangelo, Maria Grazia ^a   Levantino, Matteo ^a   Vitrano, Eugenio ^a   Cupane, Antonio ^a  

^a UNIVERSITY OF PALERMO   (Italy)

Author keywords

Optical absorption spectroscopy; Protein dynamics; Sol gel encapsulation

Indexed keywords

CYTOCHROME C; SILICON DIOXIDE; SOLVENT; WATER;

ABSORPTION SPECTROSCOPY; ANIMAL TISSUE; ARTICLE; BINDING SITE; CORRELATION ANALYSIS; ENCAPSULATION; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; FREEZING; HYDROGEL; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; SAMPLE; SOLID; STRUCTURE ANALYSIS; TEMPERATURE; WATER CONTENT; WATER STRUCTURE;

EID: 0037425464     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-4622(02)00235-1     Document Type: Article

References (31)

1. Ellerby L.M., Nishida C.R., Nishida F., et al. Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method. Science. 255:1992;1113-1115.
2. Avnir D., Braun S., Lev O., Ottolenghi M. Enzymes and other proteins entrapped in sol-gel materials. Chem. Mater. 6:1994;1605-1614.
3. Dave B.C., Dunn B., Valentine J.S., Zink J.I. Sol-gel encapsulation method for biosensors. Anal. Chem. 66:1994;1120A-1127A.
4. Savini I., Santucci R., Di Venere A., et al. Catalytic and spectroscopic properties of cytochrome-c, horseradish peroxidase, and ascorbate oxidase embedded in a sol-gel silica matrix as a function of gelation time. Appl. Biochem. Biotechnol. 82:1999;227-241.
5. Lan E.H., Dave B.C., Fukuto J.M., Dunn B., Zink J.I., Valentine J.S. Synthesis of sol-gel encapsulated heme proteins with chemical sensing properties. J. Chem. Mater. 9:1999;45-53.
6. Shibayama N., Saigo S. Fixation of the quaternary structures of human adult haemoglobin by encapsulation in transparent porous silica gels. J. Mol. Biol. 251:1995;203-209.
7. Bettati S., Mozzarelli A. T state hemoglobin binds oxygen noncooperatively with allosteric effects of protons, inositol hexaphosphate and chloride. J. Biol. Chem. 272:1997;32050-32055.
8. Shibayama N. Kinetics of the allosteric transition in hemoglobin within silicate sol-gel. J. Am. Chem. Soc. 121:1999;444-445.
9. Khan I., Shannon C.F., Dantsker D., Friedman A.J., Perez-Gonzalez-de-Apodaca J., Friedman J.M. Sol-gel trapping of functional intermediates of hemoglobin: geminate and bimolecular recombination studies. Biochemistry. 39:2000;16099-16109.
10. Bruno S., Bonnaccio M., Bettati S., et al. High and low oxygen affinity conformations of T state hemoglobin. Protein Sci. 10:2001;2401-2407.
11. Samuni U., Navati M.S., Juszczak L.J., Dantsker D., Yang M., Friedman J.M. Unfolding and refolding of sol-gel encapsulated carbonmonoxymyoglobin: an orchestrated spectroscopic study of intermediates and kinetics. J. Phys. Chem. B. 104:2000;10802-10813.
12. Frauenfelder H., Parak F., Young R.D. Conformational substates in proteins. Ann. Rev. Biophys. Chem. 17:1988;451-479.
13. Steinbach P.J., Ansari A., Berendzen J., et al. Ligand binding to heme proteins: connection between dynamics and function. Biochemistry. 30:1991;3988-4001.
14. Di Iorio E.E., Hiltpold U.R., Filipovic D., et al. Protein dynamics. Comparative investigation on heme-proteins with different physiological roles. Biophys. J. 59:1991;742-754.
15. Cameron C.E., Benkovic S.J. Evidence for a functional role of the dynamics of glycine-121 of Escherichia coli dihydrofolate reductase obtained from kinetic analysis of a site-directed mutant. Biochemistry. 36:1997;15792-15800.
16. Falconi M., Stroppolo M.E., Cioni P., et al. Dynamics-function correlation in Cu, Zn superoxide dismutase: a spectroscopic and molecular dynamics simulation study. Biophys. J. 80:2001;2556-2567.
17. Di Pace A., Cupane A., Leone M., Vitrano E., Cordone L. Protein dynamics. Vibrational coupling, spectral broadening mechanisms and anharmonicity effects in carbonmonoxy heme proteins studied by the temperature dependence of the Soret band lineshape. Biophys. J. 63:1992;475-484.
18. Cupane A., Leone M., Vitrano E., Cordone L. Low temperature optical absorption spectroscopy: an approach to the study of stereodynamic properties of hemeproteins. Eur. Biophys. J. 23:1995;385-398.
19. Melchers B., Knapp E.W., Parak F., Cordone L., Cupane A., Leone M. Structural fluctuations of myoglobin from normal-modes, Mossbauer, Raman and absorption spectroscopy. Biophys. J. 70:1996;2092-2099.
20. Sanfratello V., Boffi A., Cupane A., Leone M. Heme symmetry, vibronic structure and dynamics in heme proteins: ferrous nicotinate horse myoglobin and soybean leghemoglobin. Biopolymers (Biospectroscopy). 57:2000;291-305.
21. A. Cupane, M. Levantino, M.G. Santangelo, Near infrared spectra of water confined in silica hydrogels in the temperature interval 365-5 K, J. Phys. Chem. B (2002) in press.
22. Militello V., Leone M., Cupane A., Santucci R., Desideri A. Local dynamic properties of the heme pocket native and solvent-induced molten-globule-like states of cytochrome c. Biophys. Chem. 97:2002;121-128.
23. Schomacker K.T., Bangcharoenpaurpong O., Champion P.M. Investigation of the Stokes and anti-Stokes resonance Raman scattering of cytochrome c. J. Chem. Phys. 80:1984;4701-4717.
24. Schomacker K.T., Champion P.M. Investigations of spectral broadening mechanisms in biomolecules. J. Chem. Phys. 84:1986;5314-5325.
25. Chan C.K., Page J.B. Temperature effects in the time correlator theory of resonance Raman scattering. J. Chem. Phys. 79:1983;5234-5250.
26. Chan C.K., Page J.B. T≠0 multimode modeling of optical absorption spectra and resonance Raman profiles. Chem. Phys. Lett. 104:1984;1609-1614.
27. Angell C.A., Rodgers V. Near infrared spectra and the disrupted network model of normal and supercooled water. J. Chem. Phys. 80:1984;6245-6252.
28. Nishikawa H. Nalwa H.S. Silicon-Based Materials and Devices. 2:2001;93-122 Academic Press, San Diego.
29. Segtnan V.H., Isaksson T., Ozaki Y. Studies on the structure of water using two-dimensional near-infrared correlation spectroscopy and principal component analysis. Anal. Chem. 73:2001;3153-3161.
30. Bizzarri A.R., Paciaroni A., Cannistraro S. Glasslike dynamical behavior of the plastocyanin hydration water. Phys. Rev. E. 62:2000;3991-3999.
31. Paciaroni A., Bizzarri A.R., Cannistraro S. Neutron scattering evidence of a boson peak in protein hydration water. Phys. Rev. E. 60:1999;2476-2479.