Pulsed addition of limiting-carbon during Aspergillus oryzae fermentation leads to improved productivity of a recombinant enzyme

Biotechnology and Bioengineering

Volumn 82, Issue 1, 2003, Pages 111-117

  Bhargava, Swapnil ^a   Wenger, Kevin S ^b   Marten, Mark R ^a  

^a UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^b Novozymes Biotech Inc   (United States)

Author keywords

Fed batch; Fermentation; Filamentous fungi; Morphology

Indexed keywords

ENZYMES; FERMENTATION; MASS TRANSFER; MORPHOLOGY; VISCOSITY;

PULSE-FED FERMENTATIONS;

BIOTECHNOLOGY;

CARBON; GLUCAN 1,4 ALPHA GLUCOSIDASE; OXYGEN; RECOMBINANT ENZYME;

ARTICLE; ASPERGILLUS ORYZAE; CHEMICAL MODIFICATION; CONTROLLED STUDY; ENZYME ACTIVITY; FED BATCH FERMENTATION; FUNGAL MORPHOLOGY; MICROFILAMENT; MYCELIUM; NONHUMAN; OXYGEN CONSUMPTION; PRODUCTIVITY; VISCOSITY;

ASPERGILLUS ORYZAE; BIOREACTORS; CARBON; CELL CULTURE TECHNIQUES; FERMENTATION; GLUCAN 1,4-ALPHA-GLUCOSIDASE; GLUCOSE; PERIODICITY; POLYSACCHARIDES; QUALITY CONTROL; RECOMBINANT PROTEINS; RESTRICTION MAPPING; RHEOLOGY; VISCOSITY;

ASPERGILLUS; ASPERGILLUS ORYZAE; FUNGI;

EID: 0037420418     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10548     Document Type: Article

References (53)

1. Abel C, Linz F, Scheper T, Schugerl, K. 1994. Transient behaviour of continuously cultivated baker's yeast during enforced variations of dissolved oxygen and glucose concentrations. J Biotechno. 33: 183-193.
2. Amanullah A, Blair R, Davies A, Riley GR, Thomas CR, Nienow AW. 1999. Effects of agitation intensity on mycelial morphology and protein production in chemostat cultures of recombinant Aspergillus oryzae. Biotechnol Bioeng 62:434-446.
3. Bainbridge BW, Bull AT, Pirt SJ, Trinci APJ. 1971. Biochemical and structural changes in non-growing maintained and autolysing cultures of Aspergillus nidulans. Trans Br Mycol Soc 56:371-385.
4. Bhargava S, Nandakumar MP, Roy A, Wenger KS, Marten MR. 2002. Pulsed feeding during fed-batch fungal fermentation leads to reduced viscosity without detrimentally affecting protein expression. Biotechnol Bioeng 81:341-347.
5. Bocking SP, Wiebe MG, Robson GD, Hansen K, Christiansen LH, Trinci APJ. 1999. Effect of branch frequency in Aspergillus oryzae on protein secretion and culture viscosity. Biotechnol Bioeng 65:638-648.
6. Brooks JD, Meers JL. 1973. The effect of discontinuous methanol addition on the growth of a carbon-limited culture of Pseudomonas. J Gen Microbiol 77:513-519.
7. Buckland BC, Gwewonyo K, DiMasi D, Hunt G, Westerfield G, Nienow, AW. 1988b. Improved performance in viscous mycelial fermentations by agitator retrofitting. Biotechnol Bioeng 31:737-742.
8. Carlsen M, Spohr AB, Nielsen J, Villadsen J. 1996. Morphology and physiology of an alpha-amylase producing strain of Aspergillus oryzae during batch cultivations. Biotechnol Bioeng 49:266-276.
9. Christensen T. 1994. Application: Aspergillus oryzae as a host for production of industrial enzymes. In: Powell KA and Renwick A, editors. The genus Aspergillus. New York: Plenum Press. p 251-260.
10. Christensen T, Woeldike H, Boel E, Mortensen SB, Hjortshoej K, Thim L, Hansen MT. 1988. High level expression of recombinant genes in Aspergillus oryzae. Biotechnology 6:1419-1422.
11. Cook PE, Campbell-Platt G. 1994. Aspergillus and fermented foods. In: Powell KA, Renwick A, and Peberdy JF, editors. The genus Aspergillus. New York: Plenum Press. p 171-188.
12. DeLeon A, Galindo E, Ramirez OT. 1995. Effect of oscillating dissolved oxygen tension on penicillin acylase production by a recombinant E. coli. In: Biochemical Engineering 3: Proceedings of 3rd Intl. Symposium on Biochemical Engineering. Stutgart, Germany. p 200-202.
13. Fowler JD, Dunlop EH. 1989. Effects of reactant heterogeneity and mixing on catabolite repression in cultures of Saccharomyces cerevisiae. Biotechnol Bioeng 33:1039-1046.
14. Heinzle E, Dunn IJ 1985. The influence of cyclic glucose feeding on a continuous baker's yeast culture. Biotechnol Lett 7:235-240.
15. Heinzle E, Nishizawa Y, Dunn IJ, Bourne JR. 1981. Dynamic and steadystate effects of cyclic feeding of oxygen and glucose in an ethanolproducing yeast culture. Ann NY Acad Sci 369:159-166.
16. Holm K. 1986. Automatic spectrometric determination of amyloglucosidase activity using p-nitrophenyl-a-D-glucopyranoside and a flow injection analyser. Analyst 111:927-929.
17. Justen P, Paul GC, Nienow AW, Thomas CR. 1998a. Dependence of Penicillium chrysogenum growth, morphology, vacuolation, and productivity in fed-batch fermentations on impeller type and agitation intensity. Biotechnol Bioeng 59:762-775.
18. Justen P, Paul GC, Nienow AW, Thomas CR. 1998b. A mathematical model for agitation-induced fragmentation of Penicillium chrysogenum. Bioproc Eng 18:7-16.
19. Kataoka H, Sato S, Mukataka S, Namiki A, Yoshimura K, Takahashi J. 1986. Effects of periodic change in pressure and dissolved oxygen concentration on the incubation characteristics of Pseudomonas aeruginosa. Biotechnol Bioeng 28:663-667.
20. Larsson G, Enfors SO. 1985. Influence of oxygen starvation on the respriatory capacity of Penicillium chrysogenum. Appl Microbiol Biotechnol 21:228-233.
21. Li ZJ, Bhargava S, Marten MR. 2002a. Measurements of fragmentation rate constant imply tensile strength of fungal hyphae can change significantly during growth. Biotechnol Lett 24:1-7.
22. Li ZJ, Shukla V, Pedersen AG, Wenger KS, Fordyce AP, Marten MR 2000. Fungal morphology and fragmentation behavior in a fed-batch Aspergillus oryzae fermentation at the production scale. Biotechnol Bioeng 70:300-312.
23. Li ZJ, Shukla V, Pedersen AG, Wenger KS, Fordyce AP, Marten MR. 2002b. Effects of increased impeller power in a production-scale Aspergillus oryzae fermentation. Biotechnol Progr 18:437-444.
24. Marten MR, Wenger KS and Khan SA 1997. Rheology, Mixing Time, and Regime Analysis for a Production-Scale Aspergillus oryzae Fermentation. In: Nienow AW, editor. Bioreactor and bioprocess fluid dynamics. Cranfield, UK: BHR Group. p 295-313.
25. McIntyre M, Berry DR, McNeil B. 1999. Response of P. chrysogenum to oxygen starvation in glucose and nitrogen limited chemostat cultures. Enzyme Microb Tech 24:447-454.
26. McIntyre M, Berry DR, McNeil B. 2000. Role of proteases in autolysis of Penicillium chrysogenum chemostat cultures in response to nutrient depletion. Appl Microbiol Biotechnol 53:235-242.
27. McIntyre M, Muller C, Dynesen J, Nielsen J. 2001. Metabolic engineering of the morphology of Aspergillus. Adv Biochem Eng Biotechnol 73: 73-103.
28. Metz B, Kossen WF, van Suijdam JC. 1979. The rheology of mould suspensions. Adv Biochem En. 11:103-156.
29. Müller C. 2001. Metabolic engineering of the morphology of Aspergillus oryzae by altering chitin synthesis. Ph.D. thesis, Denmark Technical University, Lyngby, Denmark.
30. Namdev PK, Irwin N, Thompson BG, Gray MR. 1993. Effect of oxygen fluctuations on recombinant Escherichia coli fermentation. Biotechnol Bioeng 41:666-670.
31. Namdev PK, Thompson BG, Gray MR. 1992. Effect of feed zone in fed-batch fermentations of Saccharomyces cerevisiae. Biotechnol Bioeng 40:235-246.
32. Olsvik E, Kristiansen B. 1994. Rheology of filamentous fermentations. Biotechnol Adv 12:1-39.
33. Olsvik ES, Kristiansen B. 1992a. Influence of oxygen tension, biomass concentration, and specific growth rate on the rheological properties of a filamentous fermentation broth. Biotechnol Bioeng 40:1293-1299.
34. Olsvik ES, Kristiansen B. 1992b. On-line rheological measurements and control in fungal fermentations. Biotechnol Bioeng 40:375-387.
35. Oosterhuis NMG, Kossen NWF, Olivier APC, Schenk ES. 1985. Scaledown and optimization studies of gluconic acid fermentation by Gluconobacter oxydans. Biotechnol Bioeng 27:711-720.
36. Paul GC, Thomas CR. 1998. Characterization of mycelial morphology using image analysis. Adv Biochem Eng 60:1-59.
37. Perez-Leblic MI, Reyes F, Lahoz R, Archer SA 1982. Autolysis of Penicillium oxalicum with special reference to its cell walls. Can J Microbiol 28:1289-1295.
38. Pickett AM, Bazin MJ, Topiwala HH. 1979. Growth and composition of Escherichia coli subjected to square-wave perturbations in nutrient supply: Effects of varying frequencies. Biotechnol Bioeng 21: 1043-1055.
39. Pitson S, Sevoiur RJ, Bott R, Stasinopoulos SJ. 1991. Production and regulation of β-glucanases in Acremonium and Cephalosporium isolates. Mycol Res 95:352-356.
40. 14C-L-aspartic acid. J Basic Microb 8:475-482.
41. Rodriguez J, Copa-Patino JL, Perez-Leblic MI. 1995. Purification and properties of a chitinase from Penicillium oxalicum autolysates. Lett Appl Microbiol 20:46-49.
42. Sahai AS, Manocha MS. 1993. Chitinases of fungi and plants: Their involvement in morphogenesis and host-parasite interaction. FEMS Microbiol Rev 11:317-338.
43. Schweder T, Kruger E, Xu B, Jurgen B, Blomsten G, Enfors S, Hecker M. 1999. Monitoring of genes that respond to process-related stress in large-scale bioprocesses. Biotechnol Bioeng 65:151-159.
44. Smith JJ, Lilly MD, Fox RI. 1990. The effect of agitation on the morphology and penicillin production of Penicillium chrysogenum. Biotechnol Bioeng 35:1011-1023.
45. Sweere APJ, Matla YA, Zandvliet J, Luyben KCAM, Kossen NWF. 1988a. Experimental simulation of glucose fluctuations: The Influence of continually changing glucose concentrations on the fed-batch baker's yeast production. Appl Microbiol Biotechnol 28:109-115.
46. Sweere APJ, Mesters JR, Janse L, Luyben KCAM, Kossen NWF. 1988b. Experimental simulation of oxygen profiles and their influence on baker's yeast production: I. One-fermentor system. Biotechnol Bioeng 31:567-578.
47. Taguchi H, Miyamoto S. 1966. Power requirement in non-newtonian fermentation broth. Biotechnol Bioeng 8:43-54.
48. van den Hondel CAMJJ, Punt PJ, Gorcom RFMv. 1992. Production of extracellular proteins by the filamentous fungus Aspergillus. Antonie van Leeuwenhoek 61:153-160.
49. van Suijdam JC, Metz B. 1981. Influence of engineering variables upon the morphology of filamentous molds. Biotechnol Bioeng 23:111-148.
50. Vardar F, Lilly MD. 1982. Effect of cycling dissolved oxygen concentrations on product formation in penicillin fermentations. Eur J Appl Microbiol Biotechnol 14:203-211.
51. Ward PP, Lo J-Y, Duke M, May GS, Headon DR, Coneely O 1992. Production of biologically active recombinant human lactoferrin in Aspergillus oryzae. Bio/Technology 10:784-789.
52. Welles JB, Blanch HW. 1976. The effect of discontinuous feeding on ethanol production by Saccharomyces cerevisiae. Biotechnol Bioeng 18:129-132.
53. Yegneswaran PK, Gray MR. 1991. Experimental simulation of dissolved oxygen fluctuations in large fermentors: Effect on Streptomyces clavuligerus. Biotechnol Bioeng 38:1203-1209.