Production of a recombinant anti-morphine-3-glucuronide single-chain variable fragment (scFv) antibody for the development of a "real-time" biosensor-based immunoassay

Journal of Immunological Methods

Volumn 276, Issue 1-2, 2003, Pages 151-161

  Dillon, Paul P ^a,b   Manning, Bernadette M ^a   Daly, Stephen J ^a,b   Killard, Anthony J ^b   O'Kennedy, Richard ^a,b  

^a DUBLIN CITY UNIVERSITY   (Ireland)

^b DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Biosensor; ELISA; Inhibition assay; Morphine 3 glucuronide; Recombinant antibody; Surface plasmon resonance

Indexed keywords

BUFFER; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MORPHINE 3 GLUCURONIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE AMPLIFICATION; IMMUNIZATION; IMMUNOASSAY; IMMUNOSENSOR; MOLECULAR CLONING; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPLEEN CELL; SURFACE PLASMON RESONANCE; URINE;

BOVINAE; ESCHERICHIA COLI;

EID: 0037407772     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00099-1     Document Type: Article

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