Activity and population structure of nitrifying bacteria in an activated-sludge reactor containing polymer beads

Environmental Microbiology

Volumn 5, Issue 4, 2003, Pages 278-286

  Tanaka, Jun Ichi ^a,b   Syutsubo, Kazuaki ^a   Watanabe, Kazuya ^a   Izumida, Hitoshi ^b   Harayama, Shigeaki ^a  

^a MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

^b KANSAI PAINT CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; BACTERIA; BIOREACTORS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; NITROGEN; OXIDATION-REDUCTION; POLYMERS; RNA, RIBOSOMAL, 16S; SEWAGE;

BACTERIA (MICROORGANISMS); BETAPROTEOBACTERIA; NITROSPIRA; PROTEOBACTERIA;

EID: 0037385510     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.2003.00409.x     Document Type: Article

References (34)

1. Amann, R.I. (1995) In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In Molecular Microbial Ecology Manual. Kluwer Academic Publishers, 3.3.6; pp. 1-15.
2. Burrell, P.C., Keller J., and Blackall, L.L. (1998) Microbiology of a nitrite-oxidizing bioreactor. Appl Environ Microbiol 64: 1878-1883.
3. Christine, H., and Sabine, K. (1998) Simultaneous nitrification/denitrification an aerobic biofilm system. Wat Sci Tech 37: 183-187.
4. Ehrich, S., Behrens, D., Lebedeva, E., Ludwig, W., and Bock, E. (1995) A new obligately chemolithoautotrophic, nitrite-oxidizing bacterium, Nitrospira moscoviensis sp. nov. and its phylogenetic relationship. Arch Microbiol 164: 16-23.
5. Fan, X.J., Urbain, V., Qian, Y., Manem, J., Ng, W.J., and Ong, S.L. (2000) Nitrification in a membrane bioreactor (MBR) for wastewater treatment. Wat Sci Tech 42: 289-294.
6. Gieseke, A., Purkhold, U., Wagner, M., Amann, R., and Schramm, A. (2001) Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm. Appl Environ Microbiol 67: 1351-1362.
7. Head, I.M., Hiorns, W.D., Embley, T.M., McCarthy A.J., and Saunders, J.R. (1993) The phylogeny of autotrophic ammonia-oxidizing bacteria as determined by analysis of 16S ribosomal gene sequences. J Gen Microbiol 139: 1147-1153.
8. Hiorns, W.D., Hastings, R.C., Head, I.M., McCarthy, A.J., Saunders, J.R, Pickup, R.W., and Hall, G.H. (1995) Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. Microbiology 141: 2793-2800.
9. Hovanec, T.A., Taylor, L.T., Blakis, A., and Delong, E.F. (1998) Nitrospira-like bacteria associated with nitrite oxidation in freshwater aquaria. Appl Environ Microbiol 64: 258-264.
10. Juretschko, S., Timmermann, G., Schmid, M., Schleifer, K.-H., Andreas, P.-R., Koops, H.-P., and Wagner, M. (1998) Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge. Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations. Appl Environ Microbiol 64: 3042-3051.
11. Karlin, S., and Altschul, S.F. (1990) Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. Proc Natl Acad Sci USA 87: 2264-2268.
12. Kokufuta, E., Shimohashi, M., and Nakamura, I. (1987) Simultaneously occurring nitrification and denitrification under oxygen gradient by polyelectrolyte complex-coimmobilized Nitrosomonas europaea and Paracoccus denitrificans cells. Biotech Bioeng 31: 382-384.
13. Kowalchuk, G.A., Stephen, J.R., de Boer, W., Prosser, J.I., Embley, T.M., and Woldendorp, J.W. (1997) Analysis of ammonia-oxidizing bacteria of the β-subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments. Appl Environ Microbiol 63: 1489-1497.
14. Lee, N., Nielsen, P.H., Andreasen, K.H., Juretschko, S., Nielsen, J.L., Schleifer, K.-H., and Wagner, M. (1999) Combination of fluorescent in situ hybridization and microautoradiography-a new tool for structure-function analyses in microbial ecology. Appl Environ Microbiol 65: 1289-1297.
15. Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208-218.
16. Mishima, K., Nishimura, T., Goi, M., and Katsukura, N. (1996) Characteristics of nitrification and denitrification of the media-anareobic-anoxic-oxic process. Wat Sci Tech 34: 137-143.
17. Mobarry, B.K., Wagner, M., Urbain, V., Rittmann, B.E., and Stahl, D.A. (1996) Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Appl Environ Microbiol 62: 2156-2162.
18. Muyzer, G., de Waal E.C. and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 56: 695-700.
19. Okabe, S., Satoh, H., and Watanabe, Y. (1999) In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes. Appl Environ Microbiol 65: 3182-3191.
20. Prosser, J.I. (1989) Autotrophic nitrification in bacteria. Adv Microb Physiol 30: 125-181.
21. Sambrook, J., Fritsch E.F., and Maniatis T. (1989) Molecular cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
22. dos Santos, V.A.P.M., Bruijnse, M., Tramper, J., and Wijffels, R.H. (1996) The majic-bead concept: an integrated approach to nitrogen removal with co-immobilized micro-organisms. Appl Microbiol Biotechnol 45: 447-453.
23. Schramm, A., Larsen, L.H., Revsbech, N.P., Amann, R., and Schleifer, K.-H. (1996) Structure and function of a nitrifying biofilm as determined by in situ hybridization and the use of microelectrodes. Appl Environ Microbiol 62: 4641-4647.
24. Schramm, A., de Beer, D., Wagner, M., and Amann, R. (1998) Identification and activity in situ of Nitrosospira and Nitrospira spp. as dominant populations in a nitrifying fluidized bed reactor. Appl Environ Microbiol 64: 3480-3485.
25. Schramm, A., de Beer, D., Gieseke, A., and Amann, R. (2000) Microenvironments and distribution of nitrifying bacteria in a membrane-bound biofilm. Environ Microbiol 2: 680-686.
26. Stephen, J.R., McCaig, A.E., Smith, Z., Prosser, J.I., and Embley, T.M. (1996) Molecular diversity of soil and marine 16S rRNA gene sequences related to β-subgroup ammonia-oxidizing bacteria. Appl Environ Microbiol 62: 4147-4154.
27. Syutsubo, K., Kishira, H., and Harayama, S. (2001) Development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading Alcanivorax strains. Environ Microbiol 3: 1-10.
28. Tagawa, T., Syutsubo, K., Sekiguchi, Y., Ohashi, A., and Harada, H. (2000) Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization. Wat Sci Tach 42: 77-82.
29. Teske, A., Alm, E., Regan, J.M., Toze, S., Rittman, B.E., and Stahl, D.A. (1994) Evolutionary relationships among ammonia- and nitrite-oxidizing bacteria. J Bacteriol 176: 6623-6630.
30. Tsuneda, S., Miyoshi, T., and Hirata, A. (2000) Tailoring of highly efficient nitrifying biofilms in fluidized bed for ammonia-rich inductrial wastewater treatment. Wat Sci Tech 42: 357-362.
31. Wagner, M., Rath, G., Koops, H.-P., Flood, J., and Amann, R. (1996) In situ analysis of nitrifying bacteria in sewage treatment plants. Wat Sci Tech 34: 237-244.
32. Watanabe, K., Watanabe, K., Kodama, Y., Syutsubo, K., and Harayama, S. (2000b) Molecular characterization of bacterial populations in petroleum-contaminated groundwater discharged from underground crude oil storage cavities. Appl Environ Microbiol 66: 4803-4809.
33. Watson, S.W., Bock, E.H., Harms, H., Koops, H.-P., and Hooper, A.B. (1989) Nitrifying bacteria, In Bergey's Manual of Systematic Bacteriology, Vol. 3. Staley, J.T., Bryant, M.P., Pfennig N., and Holt, J.G. (eds). Baltimore, MD: The Williams & Wilkins Company, pp.1808-1834.
34. von Wintzingerode, F., Gobel, U.B., and Stackebrandt, E. (1997) Determination of microbial diversity in environmental sam-ples: pitfalls of PCR-based rRNA analysis. FEMS Microbiol Rev 21: 213-229.