Multi-photon microscopic evaluation of saphenous vein endothelium and its preservation with a new solution, GALA

Annals of Thoracic Surgery

Volumn 75, Issue 4, 2003, Pages 1145-1152

  Thatte, Hemant S ^a   Biswas, Kunda S ^a   Najjar, Samer F ^a   Birjiniuk, Vladimir ^a   Crittenden, Michael D ^a   Michel, Thomas ^a   Khuri, Shukri F ^a,b  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b VA BOSTON HEALTHCARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; ASCORBIC ACID; GLUTATHIONE; NITRIC OXIDE;

ADULT; AGED; ARTICLE; CALCIUM MOBILIZATION; CELL VIABILITY; CONTROLLED STUDY; CORONARY ARTERY BYPASS GRAFT; ENDOTHELIUM CELL; EVALUATION; FLUORESCENCE; GRAFT PATENCY; GRAFT PRESERVATION; HUMAN; HUMAN TISSUE; MALE; MICROSCOPY; PHOTON; PRESERVATION; PRIORITY JOURNAL; SAPHENOUS VEIN GRAFT; VASCULAR ENDOTHELIUM;

AGED; CELL SURVIVAL; CORONARY ARTERY BYPASS; ENDOTHELIUM, VASCULAR; HUMANS; MALE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; ORGAN PRESERVATION SOLUTIONS; PEPTIDES; SAPHENOUS VEIN;

EID: 0037383581     PISSN: 00034975     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-4975(02)04705-7     Document Type: Article

References (25)

1. Sharma GVRK, Deupree RH, Khuri SF, et al. Coronary bypass graft surgery improves survival in high risk unstable angina: results of a VA co-operative study with a eight year followup. Circulation 1991;84:260-7.
2. Mills NL, Everson CT. Vein graft failure. Curr Opin Cardiol 1995;10:562-8.
3. Davies MG, Hagen PO. Pathophysiology of vein graft failure: a review. Eur J Vasc Endovasc Surg 1995;9:7-18.
4. Edmunds LH. Techniques of myocardial revascularization. In: Edmunds LH, ed. Cardiac surgery in the adult. New York: McGraw-Hill, 1997:481-534.
5. Thatte HS, Khuri SK. The coronary artery bypass conduit: I. Intraoperative endothelial injury and its implication on graft patency. Ann Thorac Surg 2001;72:S2245-52.
6. Sellke FW, Boyle EM Jr, Verrier ED. Endothelial cell injury in cardiovascular surgery: the pathophysiology of vasomotor dysfunction. Ann Thorac Surg 1996;62:1222-8.
7. Anastasiou N, Allen S, Paniagua R, et al. Altered endothelial and smooth muscle cell reactivity caused by University of Wisconsin preservation solution in human saphenous vein. J Vasc Surg 1997;25:713-21.
8. Wagner R. Intimal protection of bypass-veins during intraoperative storage in blood or Euro-Collins solution: the role of medium, temperature, and time. J Thorac Cardiovasc Surg 1990;38:151-6.
9. Denk W, Strickler JH, Webb WW. Two-photon laser scanning fluorescence microscopy. Science 1990;248:73-6.
10. Masters BR, So PT, Gratton E. Multi-photon excitation fluorescence microscopy and spectroscopy of in vivo human skin. Biophys J 1997;72:2405-12.
11. Centonze VE, White JG. Multi-photon excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J 1998;75:2015-24.
12. Goetz RM, Thatte HS, Prabhakar P, et al. Estradiol induces the calcium-dependent translocation of endothelial nitric oxide synthase. Proc Natl Acad Sci 1999;96:2788-93.
13. Nakatsubo N, Kojima H, Kikuchi K, et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins. FEBS Lett 1998;427:263-6.
14. Biswas KS, Thatte HS, Najjar SF, et al. Multi-photon microscopy in the evaluation of human saphenous vein. J Surg Res 2001;95:37-43.
15. Monette R, Small DL, Mealing G, et al. A fluorescence confocal assay to assess neuronal viability, in brain slices. Brain Res Protoc 1998;2:99-108.
16. Hahnel C, Somodi S, Weiss DG, et al. The keratocyte network of human cornea: a three-dimensional study using confocal laser scanning fluorescence microscopy. Cornea 2000;19:185-93.
17. Angelini GD, Christie MI, Bryan AJ, Lewis MJ. Surgical preparation impairs release of endothelium derived relaxing factor from human saphenous veins. Ann Thorac Surg 1989;48:417-21.
18. Loscalzo J, Welch G. Nitric oxide and its role in the cardiovascular system. Prog Cardiovasc Dis 1995;38:87-104.
19. Michel T, Feron OJ. Nitric oxide synthase: which, where, how and why? J Clin Invest 1997;100:2146-52.
20. Siow RC, Sato H, Leake DS. Vitamin C protects human arterial smooth muscle cells against atherogenic lipoproteins: effects of antioxidant vitamin C and E on oxidized LDL induced adaptive increases in cystine transport and glutathione. Arterioscler Thromb Vasc Biol 1998;18:1662-70.
21. Dimmeler S, Zeiher AM. Endothelial cell apoptosis in angiogenesis and vessel regression. Cir Res 2000;87:434-9.
22. Cavallari N, Abebe W, Mingoli A, et al. Functional and morphological evaluation of canine veins following preservation in different storage media. J Surg Res 1997;68: 106-15.
23. Tomasian D, Keaney JF, Vita JA. Antioxidants and bioactivity of endothelial derived nitric oxide. Cardiovasc Res 2000;47: 426-35.
24. Hofman H, Schmidt HH. Thiol dependence of nitric oxide synthase. Biochemistry 1995;34:13443-52.
25. Heller R, Munscher-Paulig F, Grabner R. L-Ascorbic acid potentiates nitric oxide synthesis in endothelial cells. J Biol Chem 1999;274:8254-60.