A fluorescence confocal assay to assess neuronal viability in brain slices

Brain Research Protocols

Volumn 2, Issue 2, 1998, Pages 99-108

  Monette, Robert ^a   Small, Daniel L ^a   Mealing, Geoff ^a   Morley, Paul ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Confocal microscopy; Fluorescence; Neuronal viability assay; Neuroprotection

Indexed keywords

CALCEIN; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM ION; ETHIDIUM;

ANIMAL CELL; ARTICLE; BRAIN HYPOXIA; BRAIN SLICE; CELL VIABILITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; EVOKED RESPONSE; HIPPOCAMPUS; HYPOGLYCEMIA; NEUROPROTECTION; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; BRAIN; CELL SURVIVAL; ELECTROPHYSIOLOGY; GLUCOSE; HIPPOCAMPUS; IMAGE PROCESSING, COMPUTER-ASSISTED; MALE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NEURONS; OXYGEN CONSUMPTION; RATS; RATS, WISTAR;

EID: 0031846572     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00020-2     Document Type: Article

References (46)

1. Armstrong D.L. The hippocampal tissue slice in animal models of CNS disorders. Neurosci. Biobehav. Rev. 15:1991;79-83.
2. Beckmann N., Bruttel K., Mir A., Rudin M. Noninvasive 3D MR microscopy as a tool in pharmacological research: application to a model of rheumatoid arthritis. Magn. Reson. Imaging. 13:1995;1013-1017.
3. Bickler P.E., Hansen B.M. α2-Adrenergic agonists reduce glutamate release and glutamate receptor-mediated calcium changes in hippocampal slices during hypoxia. Neuropharmacology. 35:1996;679-687.
4. Choi D.W. Excitotoxic cell death. J. Neurobiol. 23:1992;1261-1276.
5. Cubells J.F., Rayport S., Rajendran G., Sulzer D.J. Methamphetamine neurotoxicity involves vacuolation of endocytic organelles and dopamine-dependent intracellular oxidative stress. Neuroscience. 14:1994;2260-2271.
6. Erecinska M., Nelson D., Dagani F., Deas J., Silver I.A. Relations between intracellular ions and energy metabolism under acidotic conditions: a study with nigericin in synaptosomes, neurons, and C6 glioma cells. J. Neurochem. 61:1993;1356-1368.
7. Fairchild M.D., Parsons J.E., Wasterlain C.G., Rinaldi P.C., Wallis R.A. A hypoxic injury potential in the hippocampal slice. Brain Res. 453:1988;357-361.
8. Gahwiler B.H. Organotypic monolayer cultures of nervous tissue. J. Neurosci. Methods. 4:1981;329-342.
9. Gahwiler B.H. Organotypic cultures of neural tissue. Trends Neurosci. 11:1988;484-489.
10. Gaugain B., Barbet J., Oberlin R., Roques B.P., Le Pecq J.B. DNA bifunctional intercalators. I. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer Biochemistry. 17:1978;5071-5078.
11. Gaugain B., Barbet J., Capelle N., Roques B.P., Le Pecq J.B. DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer Biochemistry. 17:1978;5078-5088.
12. Glazer A.N., Peck K., Mathies R.A. A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels. Proc. Natl. Acad. Sci. USA. 87:1990;3851-3855.
13. G.G. Glenner, Tetrazolium salts, in: R.D. Little (Ed.), H.J. Conn's Biological Stains, 8th ed., Williams and Wilkins, Baltimore, MD, 1969, pp. 154-162.
14. M.P. Goldberg, U. Strasser, L.L. Dugan, Techniques for assessing neuroprotective drugs in vitro, in: A.R. Green, A.J. Cross (Eds.), Neuroprotective Agents and Cerebral Ischemia, Academic Press, San Diego, CA, 1997, pp. 69-93.
15. A.R. Green, A.J. Cross (Eds.), Neuroprotective Agents and Cerebral Ischemia, Academic Press, San Diego, CA, 1997.
16. Gross J., Ungethum U., Andreeva N., Heldt J., Priem F., Marschhausen G., Altmann T. Glutamate-induced efflux of protein, neuron-specific enolase and lactate dehydrogenase from a mesencephalic cell culture. Eur. J. Clin. Chem. Clin. Biochem. 34:1996;305-310.
17. R.P. Haugland, Assays for cell viability, proliferation and function, in: M.T.Z. Spence (Ed.), Molecular Probes Handbook of Fluorescent Probes and Research Chemicals, 6th ed., Molecular Probes, Eugene, OR, 1996, pp. 366-398.
18. Kauppinen R.A., Kokko H., Williams S.R. Detection of mobile proteins by proton nuclear magnetic resonance spectroscopy in the guinea pig brain ex vivo and their partial purification. J. Neurochem. 58:1992;967-974.
19. Kawai S., Yonetani M., Nakamura H., Okada Y. Effects of deprivation of oxygen and glucose on the neural activity and the level of high energy phosphates in the hippocampal slices of immature and adult rat. Dev. Brain Res. 48:1989;11-18.
20. Koh J.Y., Choi D.W. Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. J. Neurosci. Methods. 20:1987;83-90.
21. Kolber M.A., Quinones R.R., Gress R.E., Henkart P.A. Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF). J. Immunol. Methods. 108:1988;255-264.
22. J. Kraicer, S.J. Dixon (Eds.), Measurement and Manipulation of Intracellular Ions, Methods in Neurosciences, Vol. 27, Academic Press, New York, 1995.
23. Krnjevic K., Morris M.E., Ropert N. Changes in free calcium ion concentration recorded inside hippocampal pyramidal cells in situ. Brain Res. 374:1986;1-11.
24. 2+ channels as drug targets for the treatment of epilepsy or ischemic stroke. Neuropharmacology. 36:1997;107-113.
25. MacManus J.P., Rasquinha I., Black M.A., Laferriere N.B., Monette R., Walker T., Morley P. Glutamate treated rat cortical neuronal cultures die in a way different from the classical apoptosis induced by staurosporine. Exp. Cell Res. 233:1997;310-320.
26. Moody G.J. Mechanistic studies of ion-selective electrodes. J. Biomed. Eng. 7:1985;183-195.
27. Nabetani M., Okada Y., Kawai S., Nakamura H. Neural activity and the levels of high energy phosphates during deprivation of oxygen and/or glucose in hippocampal slices of immature and adult rats. Int. J. Dev. Neurosci. 13:1995;3-12.
28. 31P) NMR spectroscopic studies in rats. Magn. Reson. Med. 18:1991;9-14.
29. Nishizaki T., Okada Y. Effects of excitatory amino acids on the oxygen consumption of hippocampal slices from the guinea pig. Brain Res. 452:1988;11-20.
30. J.B. Pawley, Handbook of Biological Confocal Microscopy, Plenum Press, New York, 1990.
31. Periasamy N., Armijo M., Verkman A.S. Picosecond rotation of small polar fluorophores in the cytosol of sea urchin eggs. Biochemistry. 30:1991;11836-11841.
32. Pringle A.K., Benham C.D., Sim L., Kennedy J., Jannotti F., Sundstrom L.E. Selective N-type calcium channel antagonist omega conotoxin MVIIA is neuroprotective against hypoxic neurodegeneration in organotypic hippocampal-slice cultures. Stroke. 27:1996;2124-2130.
33. Rudin M., Beckmann N., Mir A., Sauter A. In vivo magnetic resonance imaging and spectroscopy in pharmacological research: assessment of morphological, physiological and metabolic effect of drugs. Eur. J. Pharm. Sci. 3:1995;255-264.
34. Schurr A., Payne R.S., Heine M.F., Rigor B.M. Hypoxia, excitotoxicity, and neuroprotection in the hippocampal slice preparation. J. Neurosci. Methods. 59:1995;129-138.
35. A. Schurr, M.B. Rigor (Eds.), Brain Slices in Basic and Clinical Research, CRC Press, Boca Raton, FL, 1995.
36. Schwartz L.M., Osborne B.A. Cell death. Methods Cell Biol. 46:1995;XV-XVIII.
37. Small D.L., Monette R., Buchan A.M., Morley P. Identification of calcium channels involved in neuronal injury in rat hippocampal slices subjected to oxygen and glucose deprivation. Brain Res. 753:1997;209-218.
38. Small D.L., Monette R., Chakravarthy B., Durkin J., Barbe G., Mealing G., Morley P., Buchan A.M. Mechanisms of 1S,3R-ACPD-induced neuroprotection in rat hippocampal slices subjected to oxygen and glucose deprivation. Neuropharmacology. 35:1996;1037-1048.
39. Small D.L., Monette R., Mealing G., Buchan A.M., Morley P. Neuroprotective effects of ω-Aga-IVA against in vitro ischaemia in the rat hippocampal slice. NeuroReport. 6:1995;1617-1620.
40. J.A. Stamford (Ed.), Monitoring Neuronal Activity. A Practical Approach, IRL Press, Oxford, 1992.
41. Strasser U., Fisher G. Quantitative measurement of neuronal degeneration in organotypic hippocampal cultures after combined oxygen/glucose deprivation. J. Neurosci. Methods. 57:1995;177-186.
42. Straus F.H., Cheronis N.D., Straus E. Demonstration of reducing systems in neoplasms and living mammalian tissues by triphenyltetrazolium chloride. Science. 108:1948;113.
43. Tanimoto M., Okada Y. The protective effect of hypothermia on hippocampal slices from guinea pig during deprivation of oxygen and glucose. Brain Res. 417:1987;239-246.
44. Vanderkooi J.M., Erecinska M., Silver I.A. Oxygen in mammalian tissue: methods of measurement and affinities of various reactions. Am. J. Physiol. 260:1991;C1131-C1150.
45. Watson G.B., Lopez O.T., Charles V.D., Lanthorn T.H. Assessment of long-term effects of transient anoxia on metabolic activity of rat hippocampal slices using triphenyltetrazolium chloride. J. Neurosci. Methods. 53:1984;203-208.
46. 51Cr-labeled targets for natural killer cytotoxicity assay. J. Immunol. Methods. 122:1989;15-24.