Dynamic fluorescence studies of β-glycosidase mutants from Sulfolobus solfataricus: Effects of single mutations on protein thermostability

Proteins: Structure, Function and Genetics

Volumn 51, Issue 1, 2003, Pages 10-20

  Bismuto, Ettore ^a   Febbraio, Ferdinando ^b   Limongelli, Simona ^b   Briante, Raffaella ^b   Nucci, Roberto ^b  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b CNR   (Italy)

Author keywords

Archaea; Frequency domain fluorometry; Multiple sequence alignment; Protein flexibility; Thermostability

Indexed keywords

AMINO ACID; CYSTEINE; GLYCOSIDASE;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARCHAEBACTERIUM; ARTICLE; CIRCULAR DICHROISM; ENZYME STRUCTURE; FLUORESCENCE; FLUOROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; SULFOLOBUS SOLFATARICUS; THERMOSTABILITY; ULTRAVIOLET RADIATION; UNINDEXED SEQUENCE;

ADAPTATION, PHYSIOLOGICAL; AMINO ACID SEQUENCE; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENZYME STABILITY; FLUORESCENCE; FLUOROMETRY; GLUCOSIDASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; TEMPERATURE; TRYPTOPHAN;

ARCHAEA; SULFOLOBUS; SULFOLOBUS SOLFATARICUS;

EID: 0037375789     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.10317     Document Type: Article

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