Bidirectional DNA unwinding by a ternary complex of T antigen, nucleolin and topoisomerase I

EMBO Reports

Volumn 4, Issue 3, 2003, Pages 263-268

  Seinsoth, Stephanie ^a,b   Uhlmann Schiffler, Heike ^a   Stahl, Hans ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; DNA; DNA TOPOISOMERASE; DOUBLE STRANDED DNA; HELICASE; HOLOENZYME; NUCLEOLIN; SINGLE STRANDED DNA; T ANTIGEN; TUMOR ANTIGEN; UNCLASSIFIED DRUG; VIRUS DNA; VIRUS LARGE T ANTIGEN;

ANTIGEN STRUCTURE; ARTICLE; BIOCHEMISTRY; BIOGENESIS; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DNA REPLICATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RIBOSOME; SIMIAN VIRUS 40; WESTERN BLOTTING; ANIMAL CELL; DNA DENATURATION; DNA REPLICATION ORIGIN; DRUG DNA BINDING; ELECTRON MICROSCOPY; FEMALE; HUMAN; HUMAN CELL; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, VIRAL, TUMOR; CELL LINE; CERCOPITHECUS AETHIOPS; COS CELLS; DNA; DNA HELICASES; DNA TOPOISOMERASES, TYPE I; HELA CELLS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PEPTIDE FRAGMENTS; PHOSPHOPROTEINS; RECOMBINANT PROTEINS; RNA-BINDING PROTEINS; SIMIAN VIRUS 40; SPODOPTERA; TRANSFECTION;

DNA VIRUSES; SIMIAE; SIMIAN VIRUS 40;

EID: 0037366704     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.embor.embor770     Document Type: Article

References (29)

1. Belenguer, P., Caizergues-Ferrer, M., Labbe, J.C., Doree, M. & Amalric, F. (1990) Mitosis-specific phosphorylation of nucleolin by p34cdc2 protein kinase. Mol. Cell. Biol., 10, 3607-3618.
2. Belgrader, P., Siegel, A.J. & Berezney, R. (1991) A comprehensive study on the isolation and characterization of the HeLa S3 nuclear matrix. J. Cell Sci., 98, 281-291.
3. Bullock, P.A. (1997) The initiation of simian virus 40 DNA replication in vitro. Crit. Rev. Biochem. Mol. Biol., 32, 503-568.
4. Cook, P.R. (1999) The organization of replication and transcription. Science, 284, 1790-1795.
5. Daniely, Y. & Borowiec, J.A. (2000) Formation of a complex between nucleolin and replication protein A after cell stress prevents initiation of DNA replication. J. Cell Biol., 149, 799-810.
6. Dickinson, L.A. & Kohwi-Shigematsu, T. (1995) Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol., 15, 456-465.
7. Du, Y.C. & Stillman, B. (2002) Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis. Cell, 109, 835-848.
8. Falaschi, A. (2000) Eukaryotic DNA replication: a model for a fixed double replisome. Trends Genet., 16, 88-92.
9. Gai, D., Roy, R., Wu, C. & Simmons, D.T. (2000) Topoisomerase I associates specifically with simian virus 40 large-T-antigen double hexamer-origin complexes. J. Virol., 74, 5224-5232.
10. Ginisty, H., Sicard, H., Roger, B. & Bouvet, P. (1999) Structure and functions of nucleolin. J. Cell Sci., 112, 761-772.
11. Gotzmann, J., Eger, A., Meissner, M., Grimm, R., Gerner, C., Sauermann, G. & Foisner, R. (1997) Two-dimensional electrophoresis reveals a nuclear matrix-associated nucleolin complex of basic isoelectric point. Electrophoresis, 18, 2645-2653.
12. Haluska, P.J., Saleem, A., Edwards, T.K. & Rubin, E.H. (1998) Interaction between the N-terminus of human topoisomerase I and SV40 large T antigen. Nucleic Acids Res., 26, 1841-1847.
13. Hanakahi, L.A., Dempsey, L.A., Li, M.J. & Maizels, N. (1997) Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1. Proc. Natl Acad. Sci. USA, 94, 3605-3610.
14. Herendeen, D. & Kelly, T.J. (1996) in SV40 DNA Replication (ed. Blow, J.J.) 29-65. Oxford University Press, NewYork, USA.
15. Kel, A.E., Kel-Margoulis, O.V., Farnham, P.J., Bartley, S.M., Wingender, E. & Zhang M.Q. (2001) Computer-assisted identification of cell cycle-related genes: new targets for E2F transcription factors. J. Mol. Biol., 309, 99-120.
16. Labib, K. & Diffley, J.F. (2001) Is the MCM2-7 complex the eukaryotic DNA replication fork helicase? Curr. Opin. Genet. Dev., 11, 64-70.
17. Ramsperger, U. & Stahl, H. (1995) Unwinding of chromatin by the SV40 large T antigen DNA helicase. EMBO J., 14, 3215-3225.
18. Scheffner, M., Knippers, R. & Stahl, H. (1989) RNA unwinding activity of the SV40 large T antigen. Cell, 57, 955-963.
19. Schirmbeck, R. & Deppert, W. (1991) Structural topography of simian virus 40 DNA replication. J. Virol., 65, 2578-2588.
20. Simmons, D.T., Melendy, T., Usher, D. & Stillman, B. (1996) Simian virus 40 large T antigen binds to topoisomerase I. Virology, 222, 365-374.
21. Smelkova, N.V. & Borowiec, J.A. (1997) Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity. J. Virol., 71, 8766-8773.
22. Srivastava, M., Fleming, P.J., Pollard, H.B. & Burns, A.L. (1989) Cloning and sequencing of the human nucleolin cDNA. FEBS Lett., 250, 99-105.
23. Strausfeld, U. & Richter, A. (1989) Simultaneous purification of DNA topoisomerase I and II from eukaryotic cells. Prep. Biochem., 19, 37-48.
24. Uhlmann-Schiffler, H., Seinsoth, S. & Stahl, H. (2002) Preformed hexamers of SV40 T antigen are active in RNA and origin-DNA unwinding. Nucleic Acids Res., 30, 3192-3201.
25. VanLoock, M.S., Alexandrov, A., Yu, X., Cozzarelli, N.R. & Egelman, E.H. (2002) SV40 large T antigen hexamer structure: domain organization and DNA-induced conformational changes. Curr. Biol., 12, 472-476.
26. Wang, Y., Guan, J., Wang, H., Wang, Y., Leeper, D. & Iliakis, G. (2001) Regulation of DNA replication after heat shock by replication protein a-nucleolin interactions. J. Biol. Chem., 276, 20579-20588.
27. Wessel, R., Schweizer, J. & Stahl, H. (1992) Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bi-directional unwinding from the viral origin of DNA replication. J. Virol., 66, 804-815.
28. Xu, X., Hamhouyia, F., Thomas, S.D., Burke, T.J., Girvan, A.C., McGregor, W.G., Trent, J.O., Miller, D.M. & Bates, P.J. (2001). Inhibition of DNA replication and induction of S phase cell cycle arrest by G-rich oligonucleotides. J. Biol. Chem., 276, 43221-43230.
29. Xue, Z., Shan, X., Lapeyre, B. & Melese, T. (1993) The amino terminus of mammalian nucleolin specifically recognizes SV40 T-antigen type nuclear localization sequences. Eur. J. Cell Biol., 62, 13-21.