Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp

European Journal of Biochemistry

Volumn 270, Issue 6, 2003, Pages 1117-1127

  Zahner, Daniel ^a   Eckhardt, Uta ^a   Petzinger, Ernst ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Aspartate; Bile acids; Glutamate; Membrane protein; P loop

Indexed keywords

AMINO ACID; ASPARTIC ACID; BILE ACID; COMPLEMENTARY DNA; COMPLEMENTARY RNA; CYSTEINE; GLUTAMIC ACID; MEMBRANE PROTEIN; PLASMID DNA; POLYPEPTIDE; TAUROCHOLIC ACID; THREONINE;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; DNA SEQUENCE; GENE EXPRESSION; IMMUNOFLUORESCENCE MICROSCOPY; ION EXCHANGE; MEMBRANE TRANSPORT; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN TRANSPORT; RAT; SITE DIRECTED MUTAGENESIS; XENOPUS LAEVIS;

ANIMALS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CHOLAGOGUES AND CHOLERETICS; CYSTEINE; GENES, REPORTER; HUMANS; IMMUNOHISTOCHEMISTRY; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OOCYTES; ORGANIC ANION TRANSPORTERS, SODIUM-DEPENDENT; PROTEIN STRUCTURE, SECONDARY; RATS; SEQUENCE ALIGNMENT; SODIUM; SYMPORTERS; TAUROCHOLIC ACID; THREONINE; XENOPUS LAEVIS;

ANIMALIA; XENOPUS LAEVIS;

EID: 0037351144     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03463.x     Document Type: Article

References (46)

1. +/bile acid cotransport system. Proc. Natl Acad. Sci. USA 88, 10629-10633.
2. +/bile acid cotransporter. J. Clin. Invest. 93, 1326-1331.
3. Hagenbuch, B. & Meier, P.J. (1996) Sinusoidal (basolateral) bile salt uptake systems of hepatocytes. Semin. Liver Dis. 16, 129-136.
4. Wong, M.H., Oelkers, P., Craddock, A.L. & Dawson, P.A. (1994) Expression cloning and characterization of the hamster ileal sodium-dependent bile acid transporter. J. Biol. Chem. 269, 1340-1347.
5. Wong, M.H., Oelkers, P. & Dawson, P.A. (1995) Identification of a mutation in the ileal sodium-dependent bile acid transporter gene that abolishes transport activity. J. Biol. Chem. 270, 27228-27234.
6. Shneider, W.L., Dawson, P.A., Christie, D.-M., Hardikar, W., Wong, M.H. & Suchy, F.J. (1995) Cloning and molecular characterisation of the ontogeny of rat ileal sodium dependent bile acid transporter. J. Clin. Invest. 95, 745-754.
7. Lazarides, K.N., Pham, L., Tietz, P., Marinelli, P.C., Levine, S., Dawson, P.A. & LaRusso, N.F. (1997) Rat cholangiocytes absorb bile acids at their apical domain via the ileal sodium-dependent bile acid transporter. J. Clin. Invest. 100, 2714-2721.
8. Lazarides, K.N., Tietz, P., Wu, T., Kip, S., Dawson, P.A. & LaRusso, N.F. (2000) Alternatic splicing of the rat sodium/bile acid transporter changes ist cellular localization and transport properties. Proc. Natl Acad. Sci. USA 97, 11092-11097.
9. Cattori, V., Eckhardt, U. & Hagenbuch, B. (1999) Molecular cloning and functional characterization of two alternatively spliced Ntcp isoforms from mouse liver. Biochim. Biophys. Acta 1445, 154-159.
10. Hagenbuch, B. (1997) Molecular properties of hepatic uptake systems for bile acids and organic anions. J. Membrane Biol. 160, 1-8.
11. +coupled bile salt transport in isolated rat hepatocytes. Am. J. Physiol. 265, G73-G80.
12. + exchanger. Eur. J. Biochem. 268, 4674-4685.
13. ++ exchanger. J. Biol. Chem. 23, 13385-13391.
14. Lanyi, J.K. (1997) Mechanism of ion transport across membranes. Bacteriorhodopsin as a prototype for proton pumps. J. Biol. Chem. 272, 31209-31212.
15. Martin, S., Botto, J.M., Vincent, J.P. & Mazella, J. (1999) Pivotal role of an aspartate residue in sodium sensitivity and coupling to G proteins ot neurotensin receptors. Mol. Pharmacol. 55, 210-215.
16. +-coupled proline uptake. Biochemistry 36, 4631-4636.
17. +/solute symport in prokaryotes. Biochim. Biophys. Acta 1505, 131-143.
18. Poolman, B., Knol, J., van der Does, C., Henderson, P.J.F., Liang, W.-J., Leblanc, G., Pourcher, T. & Mus-Veteau, I. (1996) Cation and sugar selectivity determinats in novel family of transport proteins. Molec. Microbiol. 19, 911-922.
19. Blumrich, M. & Petzinger, E. (1990) Membrane transport of conjugated and unconjugated bile acids into hepatocytes is susceptible to SH-blocking reagents. Biochim. Biophys. Acta 1029, 1-12.
20. Blumrich, M. & Petzinger, E. (1993) Two distinct types of SH-groups are necessary for bumetanide and bile acid uptake into isolated rat hepatocytes. Biochim. Biophys. Acta 1149, 278-284.
21. Hallén, S., Fryklund, J. & Sachs, G. (2000) Inhibition of the human sodium/bile acid cotransporters by side-specific methanethiosulfonate sulfhydryl reagents: substrate-controlled accessibility of site of inactivation. Biochemistry 39, 6743-6750.
22. Mukhopadhayay, S., Ananthanarayanan, M., Stieger, B., Meier, P.J., Suchy, F.J. & Anwer, M.S. (1998) Sodium taurocholate cotransporting polypeptide is a serine, threonine phosphorprotein and is dephosphorylated by cyclic AMP. Hepatology 28, 1629-1636.
23. +/taurocholate cotransport by cyclic AMP. J. Biol. Chem. 268, 17743-17741.
24. Stengelin, S., Becker, W., Maier, M., Noll, R. & Kramer, W. (1998) Rabbit cDNA encoding hepatic sodium-dependent bile acid transporter. GeneBank accession number AJ131361.
25. Saeki, T., Motoba, K., Furukawa, H., Kirifiji, K., Kanamoto, R. & Iwami, K. (1999) Characterisation, cDNA cloning, and functional expression of mouse ileal sodium-dependent bile acid transporter. J. Biochem. (Tokyo) 125, 846-851.
26. Stengelin, S., Apel, S., Becker, W., Maier, M., Rosenberger, J., Wess, G. & Kramer, W. (1997) Ileal sodium/bile acid cotransporter; direct submission, GeneBank accession number Q28727.
27. Craddock, A.L., Love, M.W., Daniel, R.W., Kirby, L.C., Walters, H.C., Wong, M.H. & Dawson, P.A. (1998) Expression and transport properties of the human ileal and renal sodium-dependent bile acid transporter. Am. J. Physiol. 274, G157-G169.
28. Eckhardt, U., Horz, J.A., Petzinger, E., Stüber, W., Reers, M., Dickneite, G., Daniel, H., Wagener, M., Hagenbuch, B., Stieger, B. & Meier, P.J. (1996) The peptide-based thrombin inhibitor CRC 220 is a new substrate of the basolateral rat liver organic anion-transporting polypeptide. Hepatology 24, 380-384.
29. Pearson, R.B. & Kemp, B.E. (1991) Proteinkinase phosphorylation site sequences and consensus specificity motifs. Tabulations. Methods Enzymol. 200, 62-82.
30. Gerloff, T., Stieger, B., Hagenbuch, B., Madon, J., Landmann, L., Roth, J., Hofmann, A.F. & Meier, P.J. (1998) The sister of P-glycoprotein represents the canalicular bile salt export pump of mammalian liver. J. Biol. Chem. 273, 10046-10050.
31. Bahar, R. & Stolz, A. (1999) Bile acid transport. Gastroenterol. Clin. North Am. 28, 27-58.
32. Petzinger, E. (1994) Transport of organic anions in the liver. Rev. Physiol. Biochem. Pharmacol. 123, 47-211.
33. Eckhardt, U., Schroeder, A., Stieger, B., Höchli, M., Landmann, L., Tynes, R., Meier, P.J. & Hagenbuch, B. (1999) Polyspecific uptake of the hepatic organic anion transporter Oatpl in stably transfected CHO cells. Am. J. Physiol. 276, G1037-G1042.
34. Trauner, M., Meier, P.J. & Boyer, J.L. (1998) Molecular pathogenesis of cholestasis. N. Engl. J. Med. 339, 1217-1227.
35. Kullak-Ublick, G.A., Beuers, U. & Paumgartner, G. (2000) Hepatobiliary transport. J. Hepatol. 32 (Suppl. 1), 3-18.
36. Strautnieks, S.S., Kagalwalla, A.F., Tanner, M.S., Knisely, A.S., Bull, L., Freimer, N., Kocoshis, S.H., Gardiner, R.M. & Thompson, R.J. (1997) Identification of a locus for progressive familial intrahepatic cholestasis PFIC2 on chromosome 2q24. Am. J. Hum. Genet. 61, 630-632.
37. Shneider, B.L., Fox, V.L., Schwarz, K.B., Watson, C.L., Ananthanarayanan, M., Thevananther, S., Christie, D.M., Hardikar, W., Setchell, K.D.R., Mieli-Vergani, G., Suchy, F.J. & Mowat, A.P. (1997) Hepatic basolateral sodium-dependent bile acid transporter expression in two unusual cases of hypercholanemia and in extrahepatic biliary atresia. Hepatology 25, 1176-1183.
38. Gartung, C., Ananthanarayanan, M., Rahman, M., Shuele, S., Nundy, S., Soroka, C. & Stolz, A. (1996) Down-regulation of expression and function of the rat liver Na/bile acid cotransporter in extrahepatic cholestasis. Gastroeneteology 110, 199-209.
39. Oelkers, P., Kirby, L.C., Heubi, J.E. & Dawson, P.A. (1997) Primary bile acid malabsorption caused by mutations in the ileal sodium-dependent bile acid transporter gene (SLC10A2). J. Clin. Invest. 99, 1880-1887.
40. Lidofsky, S.D., Fitz, J.G., Weisiger, R.A. & Scharschmidt, B.F. (1993) Hepatic taurocholate uptake is electrogenic and influenced by transmembrane potential difference. Am. J. Physiol. 264 (Gastrointest. Liver Physiol. 27), G478-G485.
41. Kirsch, G.E., Pascual, J.M. & Shieh, C.C. (1995) Functional role of a conserved aspartate in the external mouth of voltage-gated potassium channels. Biophys. J. 68, 1804-1813.
42. +/bile acid cotransporter. Importance of the cytoplasmic tail to function and plasma membrane targeting. J. Biol. Chem. 276, 6825-6833.
43. Sun, A.Q., Ananthanarayanan, M., Soroka, C.J., Thevananther, S., Shneider, B.L. & Suchy, F.J. (1998) Sorting of rat liver ileal sodium-dependent bile acid transporters in poilarized epithelial cells. Am. J. Physiol. 275, G1045-G1055.
44. +-coupled bile acid transporters. Yale J. Biol. Med. 70, 331-340.
45. Edmondson, J.W., Miller, B.A. & Lumeng, L. (1985) Effect of glucagon on hepatic taurocholate uptake: relationship to membrane potential. Am. J. Physiol. 249 (Gastrointest. Liver Physiol. 12), G427-G433.
46. Weinman, S.A., Carruth, M.W. & Dawson, P.A. (1998) Bile acid uptake via human apical sodium-bile acid cotransporter is electrogenic. J. Biol. Chem. 273, 34691-34695.