DiscernArray™ technology: A cell-free method for the generation of protein arrays from PCR DNA

Journal of Immunological Methods

Volumn 274, Issue 1-2, 2003, Pages 265-270

  He, Mingyue ^a   Taussig, Michael J ^b  

^a Discerna Ltd   (United Kingdom)

^b BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

Cell free expression; Luciferase; Protein arrays; Protein immobilisation; Single chain antibody fragment

Indexed keywords

PROTEIN; DNA; IMMUNOGLOBULIN FRAGMENT; LUCIFERASE;

ARTICLE; CELL FREE SYSTEM; DNA MICROARRAY; IN VITRO STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN IMMOBILIZATION; PROTEIN SYNTHESIS; SURFACE PROPERTY; ANTIBODY SPECIFICITY; BIOTECHNOLOGY; GENETICS; HUMAN; IMMUNOLOGY; METHODOLOGY; PROTEIN MICROARRAY; TIME;

ANTIBODY SPECIFICITY; BIOTECHNOLOGY; DNA; HUMANS; IMMUNOGLOBULIN FRAGMENTS; LUCIFERASES; POLYMERASE CHAIN REACTION; PROTEIN ARRAY ANALYSIS; TIME FACTORS;

EID: 0037332583     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(02)00521-5     Document Type: Article

References (9)

1. Chekulayeva M.N., Kurnasov O.V., Shirokov V.A., Spirin A.S. Continuous-exchange cell-free protein-synthesizing system: synthesis of HIV-1 antigen Nef. Biochem. Biophys. Res. Commun. 280:2001;914-917.
2. He, M., Taussig, M.J., 2000. Functional protein arrays. International Patent Application (PCT/GB01/03657).
3. He M., Taussig M.J. Single step generation of protein arrays from DNA by cell-free expression and in situ immobilization (PISA method). Nucleic Acids Res. 29:2001;e73.
4. He M., Menges M., Groves M.A.T., Corps E., Liu H., Bruggemann M., Taussig M.J. Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display. J. Immunol. Methods. 231:1999;105-117.
5. Madin K., Sawasaki T., Ogasawara T., Endo Y. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc. Natl. Acad. Sci. U. S. A. 97:2000;559-564.
6. Pandey A., Mann M. Proteomics to study genes and genomes. Nature. 405:2000;837-846.
7. Robinson C.R., Sauer R.T. Optimizing the stability of single-chain proteins by linker length and composition mutagenesis. Proc. Natl. Acad. Sci. U. S. A. 95:1998;5929-5934.
8. Stevens R.C. Design of high-throughput methods of protein production for structural biology. Struct. Fold. Des. 8:2000;R177-R185.
9. Taussig M.J., Groves M.A., Menges M., Liu H., He M. Monoclonal Antibodies: A Practical Approach. 2000;91-109 Oxford Univ. Press, Oxford, UK.