Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance

Journal of Antimicrobial Chemotherapy

Volumn 51, Issue 2, 2003, Pages 247-255

  Yoshida, Reiko ^a   Kuwahara Arai, Kyoto ^a   Baba, Tadashi ^a   Cui, Longzhu ^a   Richardson, Judith F ^b   Hiramatsu, Keiichi ^a  

^a JUNTENDO UNIVERSITY   (Japan)

^b CENTRAL PUBLIC HEALTH LABORATORY   (United Kingdom)

Author keywords

Autolysis; mecA; MRSA; PBP2; PBP2

Indexed keywords

BACTERIAL ENZYME; CLAVULANIC ACID; METICILLIN; OXACILLIN; PENICILLIN BINDING PROTEIN; PENICILLINASE; TEICOPLANIN; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATE; CELL WALL; CONTROLLED STUDY; CORRELATION ANALYSIS; CULTURE MEDIUM; ELECTRON MICROSCOPY; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC CODE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; REVIEW; UNITED KINGDOM;

ACETYLGLUCOSAMINE; ANTI-BACTERIAL AGENTS; AUTOLYSIS; BACTERIAL PROTEINS; BETA-LACTAMASES; BLOTTING, WESTERN; CARRIER PROTEINS; CELL WALL; HEXOSYLTRANSFERASES; IN SITU HYBRIDIZATION; METHICILLIN; METHICILLIN RESISTANCE; MICROBIAL SENSITIVITY TESTS; MICROSCOPY, ELECTRON; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; PENICILLIN-BINDING PROTEINS; PENICILLINASE; PENICILLINS; PEPTIDYL TRANSFERASES; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 0037323798     PISSN: 03057453     EISSN: None     Source Type: Journal    
DOI: 10.1093/jac/dkg036     Document Type: Review

References (38)

1. Jevons, M. P. (1961). 'Celbenin'-resistant staphylococci. British Medical Journal 1, 124-5.
2. Reynolds, P. E. & Brown, D. F. J. (1985). Penicillin-binding proteins of beta-lactam-resistant strains of Staphylococcus aureus. FEBS Letters 192, 28-32.
3. Utsui, Y. & Yokota, T. (1985). Role of an altered penicillin-binding protein in methicillin-and cephem-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 28, 397-403.
4. Chambers, H. F., Hartman, B. J. & Tomasz, A. (1985). Increased amounts of a novel penicillin-binding protein in a strain of methicillin-resistant Staphylococcus aureus exposed to nafcillin. Journal of Clinical Investigation 76, 325-31.
5. Hiramatsu, K. (1995). Molecular evolution of MRSA. Microbiology and Immunology 39, 531-43.
6. Ito, T., Katayama, Y., Asada, K., Mori, N., Tsutsumimoto, K., Tiensasitorn, C. & Hiramatsu, K. (2001). Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 45, 1323-36.
7. Ito, T., Katayama, Y. & Hiramatsu, K. (1999). Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrobial Agents and Chemotherapy 43, 1449-58.
8. Matsuhashi, M., Song, M. D., Ishino, F., Wachi, M., Doi, M., Inoue, M. et al. (1986). Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus. Journal of Bacteriology 167, 975-80.
9. Hiramatsu, K., Kihara, H. & Yokota, T. (1992). Analysis of borderline-resistant strains of methicillin-resistant Staphylococcus aureus using polymerase chain reaction. Microbiology and Immunology 36, 445-53.
10. Murakami, K., Minamide, W., Wada, K., Nakamura, E., Teraoka, H. & Watanabe, S. (1991). Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. Journal of Clinical Microbiology 29, 2240-4.
11. Ubukata, K., Nakagami, S., Nitta, A., Yamane, A., Kawakami, S., Sugiura, M. et al. (1992). Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products. Journal of Clinical Microbiology 30, 1728-33.
12. McDougal, L. K. & Thornsberry, C. (1986). The role of β-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins. Journal of Clinical Microbiology 23, 832-9.
13. Tomasz, A., Drugeon, H. B., de Lencastre, F. H., Jabes, D., McDougal, L. & Bille, J. (1989). New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Antimicrobial Agents and Chemotherapy 33, 1869-74.
14. Hackbarth, C. J., Kocagoz, T., Kocagoz, S. & Chambers, H. F. (1995). Point mutation in Staphylococcus aureus PBP2 gene affect penicillin-binding kinetics and are associated with resistance. Antimicrobial Agents and Chemotherapy 39, 103-6.
15. Chambers, H. F., Sachdeva, M. J. & Hackbarth, C. J. (1994). Kinetics of penicillin binding proteins of Staphylococcus aureus. Biochemical Journal 301, 139-44.
16. Berger-Bächi, B., Strässle, A. & Kayser, F. H. (1989). Natural methicillin resistance in comparison with that selected by in-vitro drug exposure in Staphylococcus aureus. Journal of Antimicrobial Chemotherapy 23, 179-88.
17. Tonin, E. & Tomasz, A. (1986). β-Lactam-specific resistant mutants of Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 30, 577-83.
18. Suzuki, E., Hiramatsu, K. & Yokota, T. (1992). Survey of methicillin-resistant strains of coagulase-negative staphylococci for mecA gene distribution. Antimicrobial Agents and Chemotherapy 36, 429-34.
19. Tomasz, A., Nachman, S. & Leaf, H. (1991). Stable classes of phenotypic expression in methicillin-resistant clinical isolates of staphylococci. Antimicrobial Agents and Chemotherapy 35, 124-9.
20. Kuwahara-Arai, K., Kondo, N., Hori, S., Tateda-Suzuki, E. & Hiramatsu. (1996). Suppression of methicillin resistance in a mecA-containing pre-methicillin-resistant Staphylococcus aureus strain is caused by the mecl-mediated repression of PBP 2′ production. Antimicrobial Agents and Chemotherapy 40, 2680-5.
21. Hanaki, H., Kuwahara-Arai, K., Boyle-Vavra, S., Daum, R. S., Labischinski, H. & Hiramatsu, K. (1998). Activated cell-wall synthesis is associated with vancomycin resistance in methicillin-resistant Staphylococcus aureus clinical strains Mu3 and Mu50. Journal of Antimicrobial Chemotherapy 42, 199-209.
22. Hiramatsu, K. (2001). Vancomycin-resistant Staphylococcus aureus: a new model of antibiotic resistance. Lancet Infectious Diseases 1, 147-55.
23. Hiramatsu, K., Suzuki, E., Takayama, H., Katayama, Y. & Yokota, T. (1990). Role of penicillinase plasmids in the stability of the mecA gene in methicillin-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 34, 600-4.
24. Katayama, Y., Ito, T. & Hiramatsu, K. (2000). A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 44, 1549-55.
25. Ross, G. W. & O'Callaghan, C. H. (1975). β-Lactamase assays. In Methods in Enzymology (Hash, J. H., Ed.), Vol. 43, pp. 69-85. Academic Press, New York, NY, USA.
26. Murakami, K., Fujimura, T. & Doi, M. (1994). Nucleotide sequence of the structural gene for the penicillin-binding protein 2 of Staphylococcus aureus and the presence of a homologous gene in other staphylococci. FEMS Microbiology Letters 117, 131-6.
27. Shockman, G. D., Conover, M. J., Kolb, J. J., Riley, L. S. & Toennies, G. (1961). Nutritional requirements for bacterial cell wall synthesis. Journal of Bacteriology 81, 44-50.
28. Hiramatsu, K., Cui, L., Kuroda, M. & Ito, T. (2001). The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends in Microbiology 9, 486-93.
29. Kondo, I. & Yoshizawa, Y. (1985). Transformation in the staphylococcal system. In The Staphylococci (Jeijaszewicz, J., Ed.), Zentralblatt Bacteriologische Supplement 14, pp. 175-6. Gustav Fischer Verlag, Stuttgart.
30. Sugai, M., Akiyama, T., Komatsuzawa, H., Miyake, Y. & Suginaka, H. (1990). Characterization of dodecylsulphate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis. Journal of Bacteriology 172, 6494-8.
31. Gustafson, J. E., Berger-Bächi, B., Strässle, A. & Wilkinson, B. J. (1992). Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 36, 566-72.
32. Gustafson, J. E. & Wilkinson, B. J. (1989). Lower autolytic activity in a homogeneous methicillin-resistant Staphylococcus aureus strain compared to derived heterogeneous-resistant and susceptible strains. FEMS Microbiology Letters 59, 107-12.
33. Maki, H., Yamaguchi, T. & Murakami, K. (1994). Cloning and characterization of a gene affecting the methicillin resistance level and the autolysis rate in Staphylococcus aureus. Journal of Bacteriology 176, 4993-5000.
34. Mani, N., Baddour, L. M., Offutt, D. Q., Vijaranakul, U., Nadakavukaren, M. J. & Jayaswal, R. K. (1994). Autolysis-defective mutant of Staphylococcus aureus: pathological considerations, genetic mapping, and electron microscopic studies. Infection and Immunity 62, 1406-9.
35. Moreira, B., Boyle-Vavra, S., deJonge, B. L. M. & Daum, R. S. (1997). Increased production of penicillin-binding protein 2, increased detection of other penicillin-proteins, and decreased coagulase activity associated with glycopeptide resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 41, 1788-93.
36. Shales, D. M., Shales, J. H., Viccent, S., Etter, L., Fey, P. D. & Goering, R. V. (1993). Teicoplanin-resistant Staphylococcus aureus expresses a novel membrane protein and increases expression of penicillin-binding protein 2 complex. Antimicrobial Agents and Chemotherapy 37, 2432-7.
37. Cui, L., Murakami, H., Kuwahara-Arai, K., Hanaki, H. & Hiramatsu, K. (2000). Contribution of a thickened cell wall and its glutamine nonamidated component to the vancomycin resistance expressed by Staphylococcus aureus Mu50. Antimicrobial Agents and Chemotherapy 44, 2276-85.
38. Kuroda, M., Ohta, T., Uchiyama, I., Baba, T., Yuzawa, H., Kobayashi, I. et al. (2001). Whole genome sequencing of methicillin-resistant Staphylococcus aureus. Lancet 357, 1225-40.