Breast milk fractions solubilize Fe(III) and enhance iron flux across Caco-2 cell

Journal of Nutrition

Volumn 133, Issue 2, 2003, Pages 449-455

  Serfass, Robert E ^a   Reddy, Manju B ^b  

^a University of Texas Medical Branch School of Medicine   (United States)

^b IOWA STATE UNIVERSITY   (United States)

Author keywords

Breast milk; Caco 2 cells; Inductively coupled plasma mass spectrometry; Iron absorption

Indexed keywords

FERRIC ION; FERROUS ASCORBATE; PANCREATIN; PEPSIN A;

ARTICLE; BILE; BIOAVAILABILITY; BREAST MILK; CELL STRAIN CACO 2; CENTRIFUGATION; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; IRON ABSORPTION; IRON TRANSPORT; MASS SPECTROMETRY; SCINTILLATION COUNTING; SOLUBILIZATION; ULTRAFILTRATION; WHEY;

EID: 0037317357     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/133.2.449     Document Type: Article

References (26)

1. Saarinen, U. M., Siimes, M. A. & Dallman, P. R. (1977) Iron absorption in infants: high bioavailability of breast milk iron as indicated by the extrinsic tag method of iron absorption and by the concentration of serum ferritin. J. Pediatr. 91: 36-39.
2. 58Fe labeled supplement. Pediatr. Res. 38: 373-378.
3. Abrams, S. A., Wen, J. & Stuff, J. E. (1996) Absorption of calcium, zinc and iron from breast milk by five-to-seven-month-old infants. Pediatr. Res. 39: 384-390.
4. McMillan, J. A., Oski, F. A., Lourie G., Tomarelli, R. M. & Landaw, S. A. (1977) Iron absorption from human milk, simulated human milk and proprietary formulas. Pediatrics 60: 896-900.
5. Lönnerdal, B. (1984) Iron and breast milk. In: Iron Nutrition in Infancy and Childhood (Stekel, A., ed.), pp. 95-117. Raven Press, New York.
6. Fransson, G. & Lönnerdal B. (1983) Distribution of trace elements and minerals in human and cow's milk. Pediatr. Res. 17: 912-915.
7. Davidsson, L., Kastenmayer, P., Yuen, M., Lönnerdal, B. & Hurrell, R. F. (1994) Influence of lactoferrin on iron absorption from human milk in infants. Pediatr. Res. 35: 117-124.
8. Alvarez-Hernandez, X., Nichols, G. M. & Glass, J. (1991) Caco-2 cell line: a system for studying intestinal iron transport across epithelial cell monolayers. Biochem. Biophys. Acta 1070: 205-208.
9. Alvarez-Hernandez, X., Smith, M. & Glass, J. (1998) The effect of apotransferrin on iron release from Caco-2 cells, an intestinal epithelial cell line. Blood 91: 3974-3979.
10. Halleux, C. & Schneider, Y. (1994) Iron absorption by Caco-2 cells cultivated in serum-free medium as in vitro model of the human intestinal epithelial barrier. J. Cell. Physiol. 158: 17-28.
11. Han, O., Failla, M. L., Hill, A. D., Morris, E. R. & Smith, J. C., Jr. (1994) Inositol phosphates inhibit uptake and transport of iron and zinc by human intestinal cell line. J. Nutr. 124: 580-587.
12. Han, O., Failla, M. L., Hill, A. D., Morris, E. R. & Smith, J. C., Jr. (1995) Reduction of Fe(III) is required for uptake of nonheme iron by Caco-2 cells. J. Nutr. 125: 1291-1299.
13. Han, O., Failla, M. L. & Smith, J. C., Jr. (1997) Transferrin-iron and proinflammatory cytokines influence iron status and apical iron transport efficiency of Caco-2 intestinal cell line. Nutr. Biochem. 8: 585-591.
14. Arrendondo, M., Orellana, A., Garate, M. A. & Núñez, M. T. (1997) Intracellular iron regulates iron absorption and IRP activity in intestinal epithelial (Caco-2) cells. Am. J. Physiol. 273 (2 Pt. 1): G275-G280.
15. Núñez, M. T., Núñez-Millacura, C., Beltran, M., Tapia, V. & Alvarez-Hernandez, X. (1997) Apotransferrin and holotransferrin undergo different endocytic cycles in intestinal epithelial (Caco-2) cells. J. Biol. Chem. 27: 19425-19428.
16. Han, O., Fleet, J. C. & Wood, R.J. (1999) Reciprocal regulation of HFE and Nramp2 gene expression by iron in human intestinal cells. J. Nutr. 129: 98-104.
17. Au, A. P. & Reddy, M. B. (2000) Caco-2 cells can be used to assess human iron bioavailability from a semipurified meal. J. Nutr. 130: 1329-1339.
18. Hamosh, M., Ellis, L. A., Pollock, D. R., Henderson, T. R. & Hamosh, P. (1996) Breastfeeding and the working mother: effect of time and temperature of short-term storage on proteolysis, lipolysis, and bacterial growth in milk. Pediatrics 97: 492-498.
19. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
20. Glahn, R. P., Wien, E. M., Van Campen, D. R. & Miller, D.D. (1996) Caco-2 cell iron uptake from meat and casein digests parallels in vivo studies: use of a novel in vitro method for rapid estimation of iron bioavailability, J. Nutr. 126: 332-339.
21. Kuchler, R.J. (1972) Design of chromatographic procedures. In: Manometric & Biochemical Techniques, 5th ed. (Umbreit, W. W., Burris, R. H. & Stauffer, J. F., eds.), p. 336. Burgess Publishing, Minneapolis.
22. Swain, J. H., Tabatabal, L. B. & Reddy, M. B. (2002) Histidine content of low-molecular-weight beef proteins influences nonheme iron bioavailability in Caco-2 cells. J. Nutr. 132: 245-251.
23. Glahn, R. P., Lai, C., Hsu, J., Thompson, J. F., Guo, M. & Van Campen, D. R. (1998) Decreased citrate improves iron availability from infant formula: application of an in vitro digestion/Caco2 cell culture model. J. Nutr. 12: 257-264.
24. Schümann, K., Elsenhans, B., Hunder, G., Strugala, G. & Forth, W. (1993) Impact of chelators on the bioavailability of iron after its uptake into the mucosa. In: Bioavailability '93 Nutritional, Chemical, and Food Processing Implications of Nutrient Availability Proceedings (Schlemmer, U., ed.), pp. 33-37. Bundesforschungsanstalt für Ernährung, Karlsruhe.
25. Heine, W. E., Klein, P. D. & Reeds, P. J. (1991) The importance of α-lactalbumin in infant nutrition. J. Nutr. 121: 277-283.
26. Van Hekken, D. L. & Thompson, M. P. (1992) Application of PhastSystem to the resolution of bovine milk proteins on urea-polyacrylamide gel electrophoresis. J. Dairy Sci. 75: 1204-1210.