The influence of glycosaminoglycans and crosslinking agents on the phenotype of hepacytes cultured on collagen gels

Human and Experimental Toxicology

Volumn 22, Issue 2, 2003, Pages 65-71

  Kataropoulou, Margarita ^a   Henderson, Catherine ^a   Grant, Helen ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

Collagen crosslinking; Cytochrome P450; Extracellular matrix; Glutathione S transferases; Glycosaminoglycans; Primary hepatocyte culture

Indexed keywords

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; 1,6 HEXANEDIAMINE; ACETOACETIC ACID DERIVATIVE; CARBOXYFLUORESCEIN DIACETATE; CHONDROITIN 6 SULFATE; COLLAGEN GEL; COLLAGENASE; CROSS LINKING REAGENT; CYTOCHROME P450; GLUTATHIONE TRANSFERASE; GLYCOSAMINOGLYCAN; TESTOSTERONE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DEATH; CELL FREE SYSTEM; CELL ISOLATION; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBUNIT; IMMUNOBLOTTING; IN VITRO STUDY; LIVER CELL CULTURE; NONHUMAN; PERFUSION; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STEROID HYDROXYLATION; TIME; TISSUE SPECIFICITY; XENOBIOTIC METABOLISM;

ANIMALS; CARBODIIMIDES; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CHONDROITIN SULFATES; COLLAGEN; CROSS-LINKING REAGENTS; CULTURE MEDIA; CYTOCHROME P-450 ENZYME SYSTEM; DIAMINES; DRUG COMBINATIONS; GELS; GLUTATHIONE TRANSFERASE; HEPATOCYTES; MALE; PHENOTYPE; RATS; RATS, SPRAGUE-DAWLEY;

ANIMALIA;

EID: 0037307444     PISSN: 09603271     EISSN: None     Source Type: Journal    
DOI: 10.1191/0960327103ht320oa     Document Type: Article

References (38)

1. Rojkind M et al. Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes. J Cell Biol 1980; 87: 255-63.
2. Bissell DM, Arenson DM, Maher JJ, Roll FJ. Support of cultured hepatocytes by a laminin-rich gel. Evidence for a functionally significant subendothelial matrix in normal rat liver. J Clin Invest 1987; 79: 801-12.
3. Bucher N, Robinson G, Farmer S. Effects of extracellular matrix on hepatocyte growth and gene expression: implications for hepatic regeneration and the repair of liver injury. Semin Liver Dis 1990; 10: 11-19.
4. Fujita M, Spray DC, Choi H, Saez JC, Watanabe T, Rosenberg LC, Hertzberg EL, Reid LM. Glycosaminoglycans and proteoglycans induce gap junction expression and restore transcription of tissue-specific mRNAs in primary liver cultures. Hepatology 1987; 7: 1-9.
5. Guguen-Guillouzo C, Clement B, Baffet G, Beaumont C, Morel-Chany E, Glaise D, Guillouzo A. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp Cell Res 1983; 143: 47-54.
6. Dunn JCY, Yarmush ML, Koebe H-G, Tompkins RG. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J 1989; 3: 174-77.
7. Dunn JCY, Tompkins RG, Yarmush ML. Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol Prog 1991; 7: 237-45.
8. Michalopoulos G, Pitot HC. Primary culture of parenchymal liver cells on collagen membranes. Exp Cell Res 1975; 94: 70-78.
9. Spence JT et al. Regulation of gene expression in primary cultures of adult rat hepatocytes on collagen gels. Ann N Y Acad Sci USA 1980; 349: 99-110.
10. Lindblad WJ, Schuetz EG, Redford KS, Guzelian PS. Hepatocellular phenotype in vitro is influenced by biophysical features of the collagenous substratum. Hepatology 1991; 13: 282-88.
11. Dunn JCY, Tompkins RG, Yarmush ML. Hepatocytes in collagen sandwich: evidence for transcriptional and translational regulation. J Cell Biol 1992; 116: 1043-53.
12. Kern A, Bader A, Pichlmayr R, Sewing K-F. Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochem Pharmacol 1997; 54: 761-72.
13. Bader A et al. Use of organotypical cultures of primary hepatocytes to analyze drug biotransformation in man and animals. Xenobiotica 1994; 24: 623-33.
14. Nakamura T, Nakayama Y, Ichihara. A. Reciprocal modulation of growth and liver functions of mature rat hepatocytes in primary culture by an extract of hepatic plasma membranes. J Biol Chem 1984; 259: 8056-58.
15. Spray DC, Fujita M, Saez JC, Choi H, Watanabe T, Hertzberg E, Rosenberg LC, Reid LM. Proteoglycans and glycosaminoglycans induce gap junction synthesis and function in primary liver cultures. J Cell Biol 1987; 105: 541-51.
16. Burke JF, Yannas IV, Quinby WC, Bondoc CC, Jung WK. Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg 1981; 194: 413-28.
17. Louie LK, Yannas IV, Hsu HP, Spector M. Healing of tendon defects implanted with a porous collagen-GAG matrix: histological evaluation. Tissue Eng 1997; 3: 187-95.
18. Nehrer S, Breinan HA, Ramappa A, Hsu HP, Minas T, Shrtkroff S, Sledge CB, Yannas IV, Spector M. Chondrocyte-seeded collagen matrices implanted in a chondral defect in a canine model. Biomaterials 1998; 19: 2313-28.
19. Menard C, Mitchell S, Spector M. Contractile behaviour of smooth muscle actin-containing osteoblasts in collagen-GAG matrices in vitro: implant-related cell contraction. Biomaterials 2000; 21: 1867-77.
20. Hayes JD, Pulford DJ. The glutathione-S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol 1995; 30: 445-600.
21. Vandenberghe Y, Fosiers A, Rogiers V, Vercruysse A. Changes in expression and 'de novo' synthesis of glutathione-S-transferase subunits in cultured adult rat hepatocytes. Biochem Pharmacol 1990; 39: 685-90.
22. Power S, Sinha C, Webber M, Manson M, Neal GE. Transformation related expression of glutathione-S-transferase P in rat liver cells. Carcinogenesis 1987; 8: 797-801.
23. Vandenberge Y, Ratanasavanh D, Glaise D, Guillouzo A. Influence of medium composition and culture conditions on glutathione-S-transferase activity in adult rat hepatocytes during culture. In vitro Cell Dev Biol 1988; 24: 281-88.
24. Wortelboer HM, Do Kruif CA, Van Iersel AAJ, Falke HE, Noordhoek J, Blaauboer BJ. The isoenzyme pattern of cytochrome P450 in rat hepatocytes in primary culture, comparing different enzyme activities in microsomal incubations and in intact monolayers. Biochem Pharmacol 1990; 40: 2525-34.
25. Nyberg SL, Shatford RA, Payne WD, Hu W-S, Cerra FB. Staining with fluorescein diacetate correlates with hepatocyte function. Biotechnol Histochem 1993; 68: 56-63.
26. Elsdale T, Bard J. Collagen substrata for studies on cell behaviour. J Cell Biol 1972; 54: 626-37.
27. Moldeus P, Hogberg J, Orrenius S. Isolation and use of liver cells. Method Enzymol 1978; 52: 60-71.
28. 5 monooxygenases and cytochrome reductases in primary cultures of hepatocytes. FEBS Lett 1985; 190: 99-103.
29. Habig WH, Jakoby WB. Assays for the differentiation of glutathione-S-transferases. Method Enzymol 1981; 77: 398-405.
30. Hayes JD, Mantle TJ. Use of immunoblot techniques to discriminate between the glutathione-S-transferase Yf, Yk, Ya, Yn/Yb and Yc subunits and to study their distribution in extrahepatic tissues. Biochem J 1986; 233: 779-88.
31. 2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1. J Biol Chem 1994; 269: 20707-17.
32. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-75.
33. Sonderfan AJ, Parkinson A. Inhibition of steroid 5 alpha-reductase and its effects on testosterone hydroxylation by rat liver microsomal cytochrome P450. Arch Biochem Biophys 1988; 265: 208-18.
34. Grant MH, Anderson K, McKay G, Wills M, Henderson C, MacDonald C. Manipulation of the phenotype of immortalized rat hepatocytes by different culture configurations and by dimethylsulphoxide. Hum Exp Toxicol 2000; 19: 309-17.
35. Koda JE, Bernfeld M. Heparan sulphate proteoglycans from mouse mammary epithelial cells. J Biol Chem 1984; 259: 11763-70.
36. Hanthamrongwit M, Reid WH, Grant MH. Chondroitin-6-sulphate incorporated into collagen gels for the growth of human keratinocytes: the effect of crosslinking agents and diamines. Biomaterials 1996; 17: 775-80.
37. Lin KH, Hino H, Maeda S, Inagaki H, Airat JV, Saito T. Albumin synthesis by rat hepatocytes cultured on collagen gels is sustained specifically by heparin. Exp Cell Res 1995; 219: 717-21.
38. San Antonio JD, Lander AD, Karnovsky MJ, Slayter HS. Mapping the heparin-binding sites on type I collagen monomers and fibrils. J Cell Biol 1994; 125: 1179-88.