Manipulation of the phenotype of immortalised rat hepatocytes by different culture configurations and by dimethyl sulphoxide

Human and Experimental Toxicology

Volumn 19, Issue 5, 2000, Pages 309-317

  Grant, M H ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

Collagen substrata; Culture configuration; Dimethyl sulphoxide; Immortalised hepatocytes

Indexed keywords

COLLAGEN; CYTOCHROME P450; DIMETHYL SULFOXIDE; ETHOXYRESORUFIN DEETHYLASE; GLUTATHIONE TRANSFERASE; ISOENZYME; TESTOSTERONE; XENOBIOTIC AGENT;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; DEALKYLATION; DNA SYNTHESIS; FILM; GEL; IN VITRO STUDY; LIVER CELL; LIVER CELL CULTURE; MALE; METABOLITE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RAT; RNA SYNTHESIS; TESTOSTERONE METABOLISM; TOXICITY TESTING; XENOBIOTIC METABOLISM;

ANIMALIA;

EID: 0033866364     PISSN: 09603271     EISSN: None     Source Type: Journal    
DOI: 10.1191/096032700678815936     Document Type: Article

References (4)

1. Grant MH et al. Studies on the maintenance of cytochromes P450 and b5, monoxygenases and cytochrome reductases in primary cultures of rat hepatocytes. FEBS Letters 1985; 190: 99-103.
2. Paine AJ. The maintenance of cytochrome P450 in rat hepatocyte culture: some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system. Chemico-Biological Interactions 1990; 74: 1-31.
3. MacDonald C, Willett B. The immortalisation of rat hepatocytes by transfection with SV40 sequences. Cytotechnology 1997; 23: 161-170.
4. Yin L, MacDonald C, Grant MH. Electroporation conditions for retention of rat hepatocyte viability. The Genetic Engineer and Biotechnologist 1996; 16: 27-34.