Expression of uracil DNA glycosylase (UDG) does not affect cellular sensitivity to thymidylate synthase (TS) inhibition

European Journal of Cancer

Volumn 39, Issue 3, 2003, Pages 378-387

  Welsh, S J ^b   Hobbs, S ^a   Aherne, G W ^a  

^a INSTITUTE OF CANCER RESEARCH   (United Kingdom)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

Raltitrexed; Thymidylate synthase; Uracil DNA glycosylase; ZD9331

Indexed keywords

PLEVITREXED; RALTITREXED; THYMIDYLATE SYNTHASE; THYMIDYLATE SYNTHASE INHIBITOR; URACIL DNA GLYCOSYLTRANSFERASE;

ARTICLE; CELL DEATH; CELL GROWTH; CELL LINE; CELL VIABILITY; DNA DAMAGE; ENZYME ACTIVITY; ENZYME INHIBITION; GROWTH INHIBITION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANTIMETABOLITES, ANTINEOPLASTIC; ANTINEOPLASTIC AGENTS; CELL SURVIVAL; COMET ASSAY; DNA DAMAGE; DNA GLYCOSYLASES; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; HUMANS; INHIBITORY CONCENTRATION 50; LUNG NEOPLASMS; N-GLYCOSYL HYDROLASES; QUINAZOLINES; THIOPHENES; THYMIDYLATE SYNTHASE; TRANSFECTION; TUMOR CELLS, CULTURED; URACIL-DNA GLYCOSIDASE;

EID: 0037304541     PISSN: 09598049     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-8049(02)00610-X     Document Type: Article

References (45)

1. Grem J.L. Fluorinated pyrimidines. Chabner B.A., Collins J.M. Cancer Chemotherapy, Principles and Practice. 1990;180-224 JB Lippincott Co, Philadelphia.
2. Gunasekara N.S., Faulds T. Raltitrexed - a review of its pharmacological properties and clinical efficacy in the management of advanced colorectal cancer. Drugs. 55:1998;423-435.
3. Jackman A.L., Kimbell R., Aherne G.W.et al. Cellular pharmacology and in vivo activity of a new anticancer agent, ZD9331. a water-soluble, nonpolyglutamatable, quinazoline-based inhibitor of thymidylate synthase Clin. Cancer Res. 3:1997;911-921.
4. Rees C., Beale P., Plummer R.et al. Phase I trial of ZD9331 given as a 30 minute infusion on days 1 and 8 with cycles repeated every 3 weeks. Ann. Oncol. S4:1998;628.
5. Aherne W., Hardcastle A., Ward E., Brown S.D., Jackman A. In vitro and in vivo pharmacology of the non-polyglutamated quinazoline antifolate thymidylate synthase (TS) inhibitor, ZD9331. Ann Oncol. 7:1996;89.
6. Webley S.D., Hardcastle A., Ladner R.D., Jackman A.L., Aherne G.W. Deoxyuridine triphosphatase (dUTPase) expression and sensitivity to the thymidylate synthase (TS) inhibitor ZD9331. Br. J. Cancer. 83:2000;792-799.
7. Webley S.D., Hardcastle A., Ladner R.D., Jackman A.C., Aherne G.W. The ability to accumulate deoxyuridine triphosphate and cellular response to thymidylate synthase (TS) inhibition. Br. J. Cancer. 85:2001;446-452.
8. Richardson C.C., Schildkraut C.L., Kornberg A. Studies on the replication of DNA by DNA polymerases. Cold Spring Harbor Symp. Quant. Biol. 28:1963;9-19.
9. Dianov G., Price A., Lindahl T. Generation of single-nucleotide repair patches following excision of uracil residues from DNA. Mol. Cell Biol. 12:1992;1605-1612.
10. Aherne G.W., Brown S.D. The role of uracil misincorporation in thymineless death. Jackman A.L. Antifolate Drugs in Cancer Therapy. 1999;409-422 Humana Press, Totowa NJ, USA.
11. El-Hajj H.H., Zhang H., Weiss B. Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli. J Bacteriol. 170:1988;1069-1075.
12. Gadsden M.H., McIntosh E.M., Game J.C., Wilson P.J., Haynes R.H. dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae. EMBO J. 12:1993;4425-4431.
13. Tye B.K., Nyman P.O., Lehman I.R., Hochhauser S., Weiss B. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Proc. Natl. Acad. Sci. 74:1977;154-157.
14. Hochhauser S.J., Weiss B. Escherichia coli mutants deficient in deoxyuridine triphosphatase. J. Bacteriol. 134:1978;157-166.
15. Goulian M., Bleile B., Tseng B.Y. The effect of methotrexate on levels of dUTP in animal cells. J Biol. Chem. 255:1980;10630-10637.
16. Goulian M., Bleile B., Tseng B.Y. Methotrexate induced misincorporation of uracil in DNA. Proc. Natl. Acad. Sci. USA. 77:1980;1956-1960.
17. Barclay B.J., Kunz B.A., Little J.G., Hayes R.H. Genetic and biochemical consequences of thymidylate stress. Can J Biochem. 60:1982;172-194.
18. Richards R.G., Sowers L.C., Laszlo J., Sedwick W.D. The occurrence and consequence of deoxyuridine in DNA. Adv. Enzyme Reg. 22:1986;157-185.
19. Canman C.E., Lawrence T.S., Shewach D.S., Tang H.Y., Maybaum J. Resistance to fluorodeoxyuridine induced DNA damage and cytotoxicity correlates with an elevation of deoxyuridine triphosphatase activity and failure to accumulate deoxyuridine triphosphate. Cancer Res. 53:1993;1-6.
20. Canman C.E., Radany E.H., Parsels L.A., Davis M.A., Lawrence T.S., Maybaum J. Induction of resistance to fluorodeoxyuridine cytotoxicity and DNA damage in human tumour cells by expression of Escherichia-coli deoxyuridine triphosphatase. Cancer Res. 54:1994;2296-2298.
21. Parsels L.A., Loney T.L., Maybaum J. Expression of E-coli dUTPase partially protects HT29 cells from DNA fragmentation and cytotoxicity induced by CB3717 or methotrexate. Proc. Am. Assoc. Cancer Res. 36:1995;406.
22. Parsels L.A., Parsels J.D., Wagner L.M., Loney T.L., Radany E.H., Maybaum J. Mechanism and pharmacological specificity of dUTPase-mediated protection from DNA damage and cytotoxicity in human tumour cells. Cancer Chemother. Pharmacol. 42:1998;357-362.
23. Dusseau C., Murray G.I., Keenan R.A., O'Kelly T., Krokan H.E., McLeod H.L. Analysis of uracil DNA glycosylase in human colorectal cancer. Int. J. Oncol. 18:2001;393-399.
24. Wardleworth J.M., Boyle F.T., Barker R.J.et al. ZD9331, the design and syntheses of a novel non-polyglutamatable thymidylate synthase inhibitor. Ann. Oncol. 5(Suppl. 5):1994;247.
25. Marsham P.R., Hughes L.R., Jackman A.L.et al. Quinazoline antifolate thymidylate synthase inhibitors. heterocyclic benzoyl ring modifications J Med. Chem. 34:1991;1594-1605.
26. Olsen L.C., Aasland R., Whittwer C.U., Kroken H.E., Helland D.E. Molecular cloning of human uracil-DNA glcosylase, a highly conserved DNA repair enzyme. EMBO J. 8:1989;3121-3125.
27. Muller-Weeks S., Mastran B., Caradonna S. The nuclear form of the highly conserved human uracil-DNA glcosylase is an Mr 36,000 phosphoprotein. J. Biol. Chem. 273:1998;21909-21917.
28. Hobbs S., Jitrapakdee S., Wallace J.C. Development of a bicistronic vector driven by the human polypeptide chain elongation factor 1alpha promoter for creation of stable mammalian cell lines that express very high levels of recombinant proteins. Biochem. Biophys. Res. Commun. 252:1998;368-372.
29. Caradonna S.J., Cheng Y. Uracil DNA-glycosylase. purification and properties of this enzyme isolated from blast cells of acute myelocytic leukemia patients J. Biol. Chem. 255:1980;2293-2300.
30. Ladner R.D., McNulty D.E., Carr S.A., Roberts G.D., Caradonna S.J. Characterisation of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase. J Biol. Chem. 271:1996;7745-7751.
31. Slupphaug G., Markusson F.H., Olsen L.C.et al. Nuclear and mitochondrial forms of human uracil-DNA glycosylase are encoded by the same gene. Nucleic Acids Res. 21:1993;2579-2584.
32. Walsh M.J., Shue G., Spidoni K., Kapoor A. E2F - 1 and a cyclin-like DNA repair enzyme, uracil-DNA glycosylase provide evidence for an autoregulatory mechanism for transcription. J. Biol. Chem. 270:1995;5289-5298.
33. Houghton J.A., Harwood F.G., Tilman D.M. Thymineless death in colon carcinoma cells is mediated via Fas signalling. Proc. Natl. Acad. Sci. USA. 94:1997;8144-8149.
34. Curtin N.J., Harris A.L., Aherne G.W. Mechanisms of cell death following thymidylate synthase inhibition. 2 deoxyuridine 5 triphosphate accumulation, DNA damage, and growth inhibition following exposure to CB3717 and dipyridamole Cancer Res. 51:1991;2346-2352.
35. Matsui S., Arredondo M.A., Wrzosek C., Rustum Y.M. DNA damage and p53 induction do not cause ZD1694-induced cell cycle arrest in human colon carcinoma cells. Cancer Res. 56:1996;4712-4723.
36. Nicholl I.D., Nealon K., Kenny M.K. Reconstitution of human base excision repair with purified proteins. Biochemistry. 36:1997;7557-7566.
37. Sedwick W.D., Kurtler M., Brown O.E. Antifolate induced misincorporation of deoxyuridine monophosphate into DNA. inhibition of high molecular weight DNA synthesis in human lymphoblastoid cells Proc. Natl. Acad. Sci. USA. 78:1981;917-921.
38. Ingraham H.A., Dickey L., Goulian M. DNA fragmentation and cytotoxicity from increased cellular deoxyuridylate. Biochemistry. 25:1986;3225-3230.
39. McIntosh E.M., Haynes R.H. dUTP pyrophosphatase as a potential target for chemotherapeutic drug development. Acta Biochim. Pol. 44:1997;159-172.
40. Taylor A.F., Weiss B. Role of exonuclease III in the base excision repair of uracil-containing DNA. J. Bacteriol. 151:1982;351-357.
41. Warner H.R., Duncan B.K., Garret C., Neuhard J. Synthesis and metabolism of uracil-containing deoxyribonucleic acid in Escherichia coli. J Bacteriol. 145:1981;687-695.
42. Nilsen H., Haushalter K.A., Robins P., Barnes D.E., Verdine G.L., Lindahl T. Excision of deaminated cytosine from the vertebrate genome: role of the SMUG1 uracil-DNA glycosylase EMBO J. 20:2001;4278-4286.
43. Neddermann P., Jiricny J. Efficient removal of uracil from G.U. mispairs by the mismatch-specific thymine DNA glycosylase from HeLa cells. Proc. Natl. Acad. Sci. USA. 91:1994;1642-1646.
44. Hendrich B., Hardelande U., Ng H.H., Jiricny J., Bird A. The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites. Nature. 401:1999;301-304.
45. Muller S., Caradonna S. Isolation and characterisation of a human cDNA encoding uracil-DNA glycosylase. Biochim. Biophys. Acta. 1088:1991;197-207.