Mechanism and pharmacological specificity of dUTPase-mediated protection from DNA damage and cytotoxicity in human tumor cells

Cancer Chemotherapy and Pharmacology

Volumn 42, Issue 5, 1998, Pages 357-362

  Parsels, Leslie A ^a   Parsels, Joshua D ^a   Wagner, Lois M ^a   Loney, Tania L ^a   Radany, Eric H ^a   Maybaum, Jonathan ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

DNA damage; dUTPase; Resistance; Thymidylate synthase; Uracil

Indexed keywords

10 PROPARGYL 5,8 DIDEAZAFOLIC ACID; CLADRIBINE; DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; FLOXURIDINE; METHOTREXATE; THYMIDYLATE SYNTHASE INHIBITOR;

ARTICLE; CELL STRAIN HT29; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; DRUG RESISTANCE; ENZYME ACTIVITY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANTINEOPLASTIC AGENTS; DNA DAMAGE; DNA FRAGMENTATION; DNA, NEOPLASM; DNA, SINGLE-STRANDED; ESCHERICHIA COLI; FOLIC ACID; HUMANS; METHOTREXATE; NUCLEIC ACID SYNTHESIS INHIBITORS; PYROPHOSPHATASES; QUINAZOLINES; THYMIDYLATE SYNTHASE; TUMOR CELLS, CULTURED;

EID: 0031691145     PISSN: 03445704     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002800050829     Document Type: Article

References (22)

1. Brown SD, Ladner R, Maybaum J, Aherne GW (1997) Deoxyuridine triphosphate (dUTP) accumulation: relevance to the cytotoxic effects of thymidylate synthase (TS) inhibitors (Abstract). Proc Am Assoc Cancer Res 38: 477
2. Brynoff K, Eliasson R, Reichard P (1978) Formation of Okazaki fragments in polyoma DNA synthesis caused by misin-corporation of uracil. Cell 13: 573
3. Canman CE, Tang H-Y, Normolle DP, Lawrence TS, Maybaum J (1992) Variations in patterns of DNA damage induced in human colorectal tumor cells by 5-fluorodeoxyuridine implications for mechanisms of resistance and cytotoxicity. Proc Natl Acad Sci USA 89: 10474
4. Canman CE, Lawrence TS, Shewach DS, Tang H-Y, Maybaum J (1993) Resistance to fluorodeoxyuridine-induced DNA damage and cytotoxicity correlates with an elevation of dUT-Pase activity and failure to accumulate dUTP. Cancer Res 53: 5219
5. Canman CE, Radany EH, Parse Is LA, Davis MA, Lawrence TS, Maybaum J (1994) Induction of resistance to fluorodeoxyuridine cytotoxicity and DNA damage in human tumor cells by expression of Escherichia coli deoxyuridinetriphosphatase. Cancer Res 54: 2296
6. Curtin NJ, Harris AL, Aherne GW (1991) Mechanism of cell death following thymidylate synthase inhibition: 2′-deoxyuridine-5′-triphosphate accumulation. DNA damage, and growth inhibition following exposure to CB3717 and dipyridamole. Cancer Res 51: 2346
7. el-Hajj HH, Zhang H, Weiss B (1988) Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli. J Bacteriol 170: 1069
8. Goulian M, Bleile B, Tseng BY (1980) The effect of methotrexate on levels of dUTP in animal cells. J Biol Chem 255: 10630
9. Goulian M, Bleile B, Tseng BY (1980) Methotrexate-induced misincorporation of uracil into DNA. Proc Natl Acad Sci USA 77: 1956
10. Grafstrom RH, Tseng BY, Goulian M (1978) The incorporation of uracil into animal cell DNA in vitro. Cell 15: 131
11. Guan KL, Dixon JE (1991) Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transterase. Anal Biochem 192: 262
12. Hakes DJ, Dixon JE (1992) New vectors for high level expression of recombinant proteins in bacteria. Anal Biochem 202: 293
13. Heidelberger C, Danenberg PV, Moran RG (1983) Fluoridated pyrimidmes and their nucleosides. Adv Enzymol Relat Areas Mol Biol 54: 58
14. Ingraham HA, Dickey, L Goulian M (1986) DNA fragmentation and cytotoxicity from increased cellular deoxyuridylate. Biochemistry 25: 3225
15. Kohn KW, Ewig AG, Erickson LC, Zwelling LA (1981) DNA repair, a laboratory manual of research procedures. Marcel Dekker, New York, p. 379
16. Li JC, Kaminskas E (1984) Accumulation of DNA strand breaks and methotrexate cytotoxicity. Proc Natl Acad Sci USA 81: 5694
17. Lindahl T (1979) DNA glycosylases, endonucleases for apurinic apyrimidinic sites, and base excision-repair. Prog Nucl Acid Res Mol Biol 22: 135
18. Markowitz D, Goff S, Bank A (1988) A safe pacakaging line for gene transfer: separating viral genes on two different plasmids. J Virol 62: 1120
19. Markowitz D, Goff S, Bank A (1988) Construction and use of a safe and efficient amphotropic packaging cell line. Virology 167: 400
20. Parsels LA, Zellars RC, Loney TL, Parsels JD, Clarke MF, Lawrence TS, Maybaum J (1997) Prevention of fluorodeoxyuridine-induced cytotoxicity and DNA damage in HT29 colon carcinoma cells by conditional expression of wild-type p53 phenotype. Mol Pharmacol 52: 600
21. Sedwick WD, Kutler M, Brown OE (1981) Antifolate-induced misincorporation of deoxyuridine monophosphate into DNA: inhibition of high molecular weight DNA synthesis in human lymphoblastoid cells. Proc Natl Acad Sci USA 78: 917
22. Washtien WL (1982) Thymidylate synthetase levels as a factor in 5-fluorodeoxyuridine and methotrexate cytotoxicity in gastrointestinal tumor cells. Mol Pharmacol 21: 723