Fluorescence detection and quantitation of recombinant proteins containing oligohistidine tag sequences directly in sodium dodecyl sulfate-polyacrylamide gels

Electrophoresis

Volumn 24, Issue 4, 2003, Pages 599-610

  Hart, Courtenay ^a   Schulenberg, Birte ^a   Diwu, Zhenjun ^a   Leung, Wai Yee ^a   Patton, Wayne F ^a  

^a MOLECULAR PROBES INC   (United States)

Author keywords

Fluorescence; Histidine tag; Recombinant proteins

Indexed keywords

AMINO ACIDS; ARGON LASERS; FLUOROPHORES; GELS; HARMONIC GENERATION; NONLINEAR OPTICS; RECOMBINANT PROTEINS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

EMISSION MAXIMA; FLUORESCENCE DETECTION; FLUORESCENCE EMISSION; FUSION PROTEINS; HISTIDINE TAGS; NITRILOTRIACETIC ACID; OLIGO-HISTIDINE; OLIGOHISTIDINE TAG; POLYACRYLAMIDE GELS; SODIUM DODECYL SULPHATE;

SAPPHIRE;

ALKALINE PHOSPHATASE; DODECYL SULFATE SODIUM; DYE; HISTIDINE DERIVATIVE; HYBRID PROTEIN; NICKEL; NITRILOTRIACETIC ACID; POLYACRYLAMIDE GEL; REAGENT; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN; STREPTAVIDIN;

AMINO ACID SEQUENCE; ARGON LASER; ARTICLE; CELL LYSATE; CONJUGATION; CONTROLLED STUDY; DIAGNOSTIC IMAGING; DIAGNOSTIC KIT; ELECTROBLOTTING; FLUORESCENCE ANALYSIS; FLUORESCENCE SPECTROSCOPY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN GEL BLOT ANALYSIS; SENSITIVITY ANALYSIS; STANDARDIZATION; ULTRAVIOLET A RADIATION; ULTRAVIOLET B RADIATION;

EID: 0037296872     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200390070     Document Type: Article

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