Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose

Analytical Biochemistry

Volumn 236, Issue 1, 1996, Pages 101-106

  McMahan, Scott A ^a   Burgess, Richard R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING SITES; CHEMICAL DETECTION; COENZYMES; ESCHERICHIA COLI; MONOCLONAL ANTIBODIES; NICKEL COMPOUNDS; NITROCELLULOSE; REAGENTS;

BIFUNCTIONAL COMPOUNDS; COORDINATION SITES; HIS-TAGGED PROTEINS; HISTIDINE-TAGGED PROTEINS; HORSE-RADISH PEROXIDASE; NITRILOTRIACETIC ACID; ONE-STEP REACTIONS; SINGLE-STEP SYNTHESIS;

BIOSYNTHESIS;

BIOTIN; CHELATING AGENT; DODECYL SULFATE SODIUM; HISTIDINE; HORSERADISH PEROXIDASE; NICKEL; NITRILOTRIACETIC ACID; PYROXYLIN; REAGENT; RNA POLYMERASE; STREPTAVIDIN;

ANIMAL CELL; ARTICLE; CHEMOLUMINESCENCE; DISSOCIATION; ESCHERICHIA COLI; HELA CELL; HUMAN; HUMAN CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; SYNTHESIS; YEAST CELL;

EID: 0029996505     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0137     Document Type: Article

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