Cloning, localization, and axonemal function of tetrahymena centrin

Molecular Biology of the Cell

Volumn 14, Issue 1, 2003, Pages 251-261

  Guerra, Charles ^a   Wada, Yuuko ^a   Leick, Vagn ^b   Bell, Aaron ^a   Satir, Peter ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CENTRIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; AXONEME; CALCIUM TRANSPORT; CHEMOTAXIS; CONTROLLED STUDY; ENZYME ACTIVITY; EUKARYOTIC FLAGELLUM; IN VITRO STUDY; KINETOSOME; MICROTUBULE; MOLECULAR CLONING; MUCOCILIARY TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SEQUENCE HOMOLOGY; TETRAHYMENA THERMOPHILA;

AMINO ACID SEQUENCE; ANIMALS; AXONS; BASE SEQUENCE; CALCIUM; CALCIUM-BINDING PROTEINS; CHROMOSOMAL PROTEINS, NON-HISTONE; EF HAND MOTIFS; MOLECULAR SEQUENCE DATA; PHYLOGENY; TETRAHYMENA; TUBULIN;

EUKARYOTA; INVERTEBRATA; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

EID: 0037241834     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E02-05-0298     Document Type: Article

References (38)

1. Asai, D.J., and Forney, J.D. (2000). Tetrahymena thermophila. In: Methods in Cell Biology, vol. 62, San Diego, CA: Academic Press, 580 pps.
2. Brokaw, C.J., and Kamiya, R. (1987). Bending patterns of Chlamydomonas flagella IV. Mutants with defects in inner and outer dynein arms indicate differences in dynein arm function. Cell Motil. Cytoskel. 8, 68-75.
3. Chomczynski, P., and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
4. Christensen, S.T., Guerra, C., Wada, Y., Valentin, T., Angeletti, R.H., Satir, P., and Hamasaki, T. (2001). A regulatory light chain of ciliary outer arm dynein in Tetrahymena thermophila. J. Biol. Chem. 276, 20048-20054.
5. Cohen, J., and Beisson, J. (1988). The Cytoskeleton. In: Paramecium, vol. 21, ed. H.D. Görtz, Heidelberg: Springer Verlag, 363-391.
6. Femino, A.M., Fay, F.S., Fogarty, K., and Singer, R.H. (1998). Visualization of single RNA transcripts in situ. Science 280, 585-590.
7. Gaertig, J., Thatcher, T.H., McGrath, K.E., Callahan, R.C., and Gorovsky, M.A. (1993). Perspectives on tubulin isotype function and evolution based on the observation that Tetrahymena thermophila microtubules contain a single alpha- and beta-tubulin. Cell Motil. Cytoskel. 25, 243-53.
8. 2+-binding proteins and contractility of the infraciliary lattice in Paramecium. Biol. Cell 71, 217-225.
9. Goodenough, U. (1983). Motile detergent-extracted cells of Tetrahymena and Chlamydomonas. J. Cell Biol. 96, 1610-1621.
10. Gorovsky, M.A. (1973). Macro- and micronuclei of Tetrahymena pyriformis: a model system for studying the structure and function of eukaryotic nuclei. J. Protozool. 20, 19-25.
11. Hamasaki, T., Holwill, M.E., Barkalow, K., and Satir, P. (1995). Mechanochemical aspects of axonemal dynein activity studied by in vitro microtubule translocation. Biophys. J. 69, 2569-2579.
12. 2+ -binding protein: homology in its protein sequence with CaM and the yeast CDC31 gene product. J. Cell Biol. 107, 133-140.
13. Jerka-Dziadosz, M., Jenkins, L.M., Nelson, E.M., Williams, N.E., Jaeckel-Williams, R., and Frankel, J. (1995). Cellular polarity in ciliates: persistence of global polarity in a disorganized mutant of Tetrahymena thermophila that disrupts cytoskeletal organization. Dev. Biol. 169, 644-661.
14. Klotz, C., Garreau de Loubresse, N., Ruiz, F., and Beisson, J. (1997). Genetic evidence for a role of centrin-associated proteins in the organization and dynamics of the infraciliary lattice in Paramecium. Cell Motil. Cytoskel. 38, 172-186.
15. Laoukili, J., Perret, E., Middendorp, S., Houcine, O., Guennou, C., Marano, F., Bornens, M., and Tournier, F. (2000). Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro. J. Cell Sci. 113, 1355-1364.
16. Larsen, J., Barkalow, K., Hamasaki, T., and Satir, P. (1991). Structural and functional characterization of Paramecium dynein: initial studies. J. Protozool. 38, 55-61.
17. LeDizet, M., Beck, J.C., and Finkbeiner, W.E. (1998). Differential regulation of centrin genes during ciliogenesis in human tracheal epithelial cells. Am. J. Physiol. 275, L1145-L1156.
18. LeDizet, M., and Piperno, G. (1995). The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes. Mol. Biol. Cell 6, 697-711.
19. Leick, V., Koppelhus, U., and Rosenberg, J. (1994). Cilia-mediated oriented chemokinesis in Tetrahymena thermophila. J. Euk. Microbiol. 41, 546-553.
20. Lutz, W., Lingle, W.L., McCormick, D., Greenwood, T.M., and Salisbury, J.L. (2001). Phosphorylation of centrin during the cell cycle and its role in centriole separation preceeding centrosome duplication. J. Biol. Chem. 276, 20774-20780.
21. Maciejewski, J.J., Vacchiano, E.J., McCutcheon, S.M., and Buhse, H.E., Jr. (1999). Cloning and expression of a cDNA encoding a Vorticella convallaria spasmin: an EF hand calcium-binding protein. J. Euk. Microbiol. 46, 165-73.
22. Madeddu, L., Klotz, C., Le Caer, J.P., and Beisson, J. (1996). Characterization of centrin genes in Paramecium. Eur. J. Biochem. 238, 121-128.
23. Maihle, N.J., and Satir, B.H. (1980). Calmodulin in the ciliates Paramecium tetraurelia and Tetrahymena thermophila. Ann. NY Acad. Sci. 356, 408-409.
24. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
25. Onimaru, H., Ohki, K., Nozawa, Y., and Naitoh, Y. (1980). Electrical properties of Tetrahymena, a suitable tool for studies on membrane excitation. Proc. Jpn. Acad. 56, 538-543.
26. Routledge, L.M. (1978). Calcium-binding proteins in the vorticellid spasmoneme. J. Cell Biol. 77, 358-370.
27. Salisbury, J.L., Baron, A.T., Surek, B., and Melkonian, M. (1984). Stritiated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle. J. Cell Biol. 99, 962-970.
28. Salisbury, J.L. (1995). Centrin, centrosomes, and meiotic spindle bodies. Curr. Opin. Cell Biol. 7, 39-45.
29. Sanders, M.A., and Salisbury, J.L. (1989). Centrin-mediated microtubule severing during flagellar excision in Chlamydomonas reinhardtii. J. Cell Biol. 108, 1751-1760.
30. Satir, B., Sale, W.S., and Satir, P. (1976). Membrane renewal after dibucaine deciliation of Tetrahymena. Freeze-fracture technique, cilia, membrane structure. Exp. Cell Res. 97, 83-91.
31. Satir, P. (1998). Mechanisms of ciliary motility: an update. Eur. J. Protistol. 34, 267-272.
32. Schliwa, M., and van Blerkom, J. (1981). Structural interaction of cytoskeletal components. J. Cell Biol. 90, 222-235.
33. Takemasa, T., Ohnishi, K., Kobayashi, T., Takagi, T., Konishi, K., and Watanabe, Y. (1989). Cloning and sequencing of the gene for Tetrahymena calcium-binding 25-kDa protein (TCBP-25). J. Biol. Chem. 264, 19293-19301.
34. Takemasa, T., Takagi, T., Kobayashi, T., Konishi, K., and Watanabe, Y. (1990). The third calmodulin family protein in Tetrahymena. Cloning of the cDNA for Tetrahymena calcium-binding protein of 23 kDa (TCBP-23). J. Biol. Chem. 265, 2514-2517.
35. Towbin, H., Staehelin, T., and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
36. Villain, M. et al. (2000). De novo design of peptides targeted to the EF hands of calmodulin. J. Biol. Chem. 275, 2676-2685.
37. Wada, Y., Hamasaki, T., and Satir, P. (2000). Evidence for a novel affinity mechanism of motor-assisted transport along microtubules. Mol. Biol. Cell 11, 161-169.
38. Yang, P., Diener, D.R., Rosenbaum, J.L., and Sale, W.S. (2001). Localization of calmodulin and dynein light chain LC8 in flagellar radial spokes. J. Cell Biol. 153, 1315-1325.