Localization of calmodulin and dynein light chain LC8 in flagellar radial spokes

Journal of Cell Biology

Volumn 153, Issue 6, 2001, Pages 1315-1325

  Yang, Pinfen ^a   Diener, Dennis R ^b   Rosenbaum, Joel L ^b   Sale, Winfield S ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b YALE UNIVERSITY   (United States)

Author keywords

Calcium; Calmodulin; Cilia; Dynein; Flagella

Indexed keywords

CALMODULIN; DYNEIN ADENOSINE TRIPHOSPHATASE;

ARTICLE; AXONEME; CHLAMYDOMONAS; ELECTRON MICROSCOPY; FLAGELLUM; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN ISOLATION;

ANIMALS; CALMODULIN; CARRIER PROTEINS; CHLAMYDOMONAS; CYSTEINE ENDOPEPTIDASES; DROSOPHILA PROTEINS; FLAGELLA; MULTIENZYME COMPLEXES; PROTEASOME ENDOPEPTIDASE COMPLEX;

CHLAMYDOMONAS;

EID: 0035844885     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.153.6.1315     Document Type: Article

References (71)

1. Calcium regulation of microtubule sliding in reactivated sea urchin sperm flagella
2. Dimerization of the highly conserved light chain shared by dynein and myosin V
3. Calcium control of waveform in isolated flagellar axonemes of Chlamydomonas
4. Regulation of sperm flagellar motility by calcium and cAMP-dependent phosphorylation
5. Control of flagellar bending: A new agenda based on dynein diversity
6. Modulation of the asymmetry of sea urchin sperm flagellar bending by calmodulin
7. Analysis of the movement of Chlamydomonas flagella: The function of the radial-spoke system is revealed by comparison of wild-type and mutant flagella
8. Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons
9. Flagellar radial spoke: A model molecular genetic system for studying organelle assembly
10. Assembly of flagellar radial spoke proteins in Chlamydomonas: Identification of the axoneme binding domain of radial spoke protein 3
11. Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii
12. The light chain composition of chicken brain myosin-Va: Calmodulin, myosin-II essential light chains, and 8-kDa dynein light chain/PIN
13. Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants
14. Flagellar radial spoke protein 3 is an A-kinase anchoring protein (AKAP)
15. Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella
16. Purification of calmodulin from Chlamydomonas: Calmodulin occurs in cell bodies and flagella
17. Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella
18. Structure of the soluble and in situ ciliary dyneins visualized by quick-freeze deep-etch microscopy
19. Regulation of flagellar dynein by an axonemal type-1 phosphatase in Chlamydomonas
20. Identification of the t complex-encoded cytoplasmic dynein light chain Tctex1 in inner arm 11 supports the involvement of flagellar dyneins in meiotic drive
21. Regulation of Chlamydomonas flagellar dynein by an axonemal protein kinase
22. Chlamydomonas reinhardtii: A model system for the genetic analysis of flagellar structure and motility
23. Radial spokes of Chlamydomonas flagella: Genetic analysis of assembly and function
24. Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for flagellar function
25. Production and specificity of monoclonal antibodies against calmodulin from Dictyostelium discoideum
26. PIN: An associated protein inhibitor of neuronal nitric oxide synthase
27. Submicromolar levels of calcium control the balance of beating between the two flagella in demembranated models of Chlamydomonas
28. The dynein microtubule motor
29. Outer arm dynein light chains from Chlamydomonas flagella have cytoplasmic homologues
30. 2+-binding light chain within Chlamydomonas outer arm dynein
31. The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein
32. Cytoplasmic dynein ATPase activity is regulated by dynactin-dependent phosphorylation
33. The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes
34. Flagellar doublet microtubules: Fractionation of minor components and α-tubulin from specific regions of the A-tubule
35. The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif.
36. Flagellar mutants of Chlamydomonas: Studies of radial spoke defective strains by dikaryon and revertant analysis
37. Amino acid sequence of a novel calmodulin from the unicellular alga Chlamydomonas
38. Arrangement of inner dynein arms in wild-type and mutant flagella of Chlamydomonas
39. Characterization of a Chlamydomonas insertional mutant that disrupts flagellar central pair microtubule-associated structures
40. Rotation of the central pair microtubules in eukaryotic flagella
41. Calmodulin and the control of flagellar movement
42. A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT)
43. Purification and polypeptide composition of dynein ATPases from Chlamydomonas flagella
44. Two-dimensional separation of axonemal proteins
45. Axonemal adenosine triphosphatases from flagella of Chlamydomonas reinhardtii
46. Radial spokes of Chlamydomonas flagella: Polypeptide composition and phosphorylation of stalk components
47. Three distinct inner dynein arms in Chlamydomonas flagella: Molecular composition and location in the axoneme
48. The 9 + 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility
49. Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms
50. Class V myosins
51. Sequence motifs for calmodulin recognition
52. Switching mechanisms in the control of ciliary motility
53. Dynein arms are oscillating force generators
54. Regulation of dynein-driven microtubule sliding by the radial spokes in flagella
55. Structural and functional reconstitution of inner dynein arms in Chlamydomonas flagellar axonemes
56. The role of central apparatus components in flagellar motility and microtubule assembly
57. Mammalian nitric oxide synthases
58. Cilia with defective radial spokes: A cause of human respiratory disease
59. Similarities and dissimilarities between calmodulin and a Chlamydomonas flagellar protein
60. 2+-dependent waveform conversion in the flagellar axoneme of Chlamydomonas mutants lacking the central-pair/radial spoke system
61. The structural basis of ciliary bend formation: Radial spoke positional changes accompanying microtubule sliding
62. Molecular cloning and expression of flagellar radial spoke and dynein genes of Chlamydomonas
63. Molecular cloning and sequence analysis of the Chlamydomonas gene coding for radial spoke protein 3: Flagellar mutation pf-14 is an ochre allele
64. Isolation of Chlamydomonas flagella and flagellar axonemes
65. Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components
66. Vigorous beating of Chlamydomonas axonemes lacking central pair/radial spoke structures in the presence of salts and organic compounds
67. r 140,000 intermediate chain of Chlamydomonas flagellar inner arm dynein is a WD-repeat protein implicated in dynein arm anchoring
68. Casein kinase I is anchored on axonemal doublet microtubules and regulates flagellar dynein phosphorylation and activity
69. Protein phosphatases PP1 and PP2A are located in distinct positions in the Chlamydomonas flagellar axoneme
70. Effects of the central pair apparatus on microtubule sliding velocity in sea urchin sperm flagella
71. Structural organization, DNA sequence, and expression of the calmodulin gene