Development of an immunomagnetic separation-polymerase chain reaction (IMS-PCR) assay specific for Enterocytozoon bieneusi in water samples

Journal of Applied Microbiology

Volumn 94, Issue 2, 2003, Pages 273-279

  Sorel, N ^a   Guillot, E ^b   Thellier, M ^c   Accoceberry, I ^d   Datry, A ^c   Mesnard Rouiller, L ^a   Miegeville M ^a  

^a NANTES UNIVERSITY HOSPITAL   (France)

^b Ctr Tech et de Rech d'ONDEO Serv   (France)

^c PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^d BORDEAUX UNIVERSITY HOSPITAL   (France)

Author keywords

Enterocytozoon bieneusi; Immunomagnetic separation; Microsporidia; PCR; Water samples

Indexed keywords

CHAINS; DIAGNOSIS; MONOCLONAL ANTIBODIES; SCREENING;

'CURRENT; DETECTION METHODS; ENTEROCYTOZOA BIENEUSI; ENVIRONMENTAL OCCURRENCE; GASTROINTESTINAL DISEASE; HUMAN PATHOGENS; IMMUNOMAGNETIC SEPARATION; MICROSPORIDIA; SPECIES SPECIFICS; WATER SAMPLES;

POLYMERASE CHAIN REACTION;

MONOCLONAL ANTIBODY;

DETECTION METHOD; MAGNETIC TECHNIQUE; MICROBIAL POLLUTANT; SEPARATION; WATER TREATMENT;

DETECTION METHOD; IMMUNOASSAY; MICROORGANISM; POLYMERASE CHAIN REACTION;

ARTICLE; CONTROLLED STUDY; CROSS REACTION; ENTEROCYTOZOON; IMMUNOMAGNETIC SEPARATION; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WATER ANALYSIS; WATER SAMPLING;

ENCEPHALITOZOON; ENTEROCYTOZOON BIENEUSI; MICROSPORIDIA; PROTOZOA;

EID: 0037238259     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2003.01830.x     Document Type: Article

References (21)

1. Accoceberry, I., Thellier, M., Desportes-Livages, I., Achbarou, A., Biligui, S., Danis, M. and Datry, A. (1999) Production of monoclonal antibodies directed against the microsporidium Enterocytozoon bieneusi. Journal of Clinical Microbiology 37, 4107-4112
2. Bailenger, J. (1965) Recherche des parasites intestinaux microscopiques-Méthode de Bailenger. In Coprologie parasitaire et fonctionnelle ed. Drouillard, E. p. 81. Bordeaux, France.
3. Bicart-Sée, A., Massip, P., Linas, M.-D. and Datry, A. (2000) Successfull treatment with nitazoxanide of Enterocytozoon bieneusi microsporidiosis in a patient with AIDS. Antimicrobial Agents and Chemotherapy 44, 167-168.
4. Da Silva, A.J., Schwartz, D.A., Visvesvara, G.S., De Moura, H., Slemanda, S.B. and Pieniazek, N.J. (1996) Sensitive PCR diagnosis of infections by Enterocytozoon bieneusi (Microsporidia) using primers based on the region coding for small-subunit rRNA. Journal of Clinical Microbiology 34, 986-987.
5. Dowd, S.E., Gerba, C.P. and Pepper, I.L. (1998) Confirmation of the human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in water. Applied And Environmental Microbiology 64, 3332-3335.
6. Dowd, S.E., Gerba, C.P., Enriquez, F.J. and Pepper, I.L. (1998) PCR amplification and species determination of microsporidia in formalin-fixed feces after immunomagnetic separation. Applied and Environmental Microbiology 64, 333-336.
7. Enriquez, F.J., Ditrich, O., Palting, J.D. and Smith, K. (1997) Simple diagnosis of Encephalitozoon sp. microsporidial infections by using a panspecific antiexospore monoclonal antibody. Journal of Clinical Microbiology 35, 724-729.
8. Fedorko, D.P., Nelson, N.A. and Cartwright, C.P. (1995) Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. Journal of Clinical Microbiology 33, 1739-1741.
9. Fournier, S., Liguory, O., Santillana-Hayat, M., Guillot, E., Sarfati, C., Dumoutier, N., Molina, J.M. and Derouin, F. (2000) Detection of microsporidia in surface water: a one-year follow-up study. FEMS Immunology and Medical Microbiology 29, 95-100.
10. Gainzarain, J.C., Canut, A., Lozano, M., Labora, A., Carreras, F., Fenoy, S., Navajas, R., Pieniazek, N.J., Da Silva, A.J. and Del Aguila, C. (1998) Detection of Enterocytozoon bieneusi in two human immunodeficiency virus-negative patients with chronic diarrhea by polymerase chain reaction in duodenal biopsy specimens and review. Clinical Infectious Disease 27, 394-398.
11. Hallier-Soulier, S. and Guillot, E. (1999) An immunomagnetic separation polymerase chain reaction assay for rapid and ultra-sensitive detection of Cryptosporidium parvum in drinking water. FEMS Microbiology Letters 176, 285-289.
12. Hallier-Soulier, S. and Guillot, E. (2000) Detection of Cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction. Journal of Applied Microbiology 89, 5-10.
13. Hollister, W.S., Canning, E.U. and Anderson, C.L. (1996) Identification of microsporidia causing human disease. Journal of Eukaryotic Microbiology 43, 104S-105S.
14. Kokoskin, E., Gyorkos, T.W., Camus, A., Cedilotte, L., Purtill, T. and Ward, B. (1994) Modified technique for efficient detection of microsporidia. Journal of Clinical Microbiology 32, 1074-1075.
15. Kotler, D.P. (1995) Gastrointestinal manifestations of immunodeficiency infection. in Internal Medicine 40, 197-241.
16. Mahbubani, M.H., Schaefer, F.W. III, Jones, D.D. and Bej, A.K. (1998) Detection of Giardia in environmental waters by immuno-PCR amplification methods. in Microbiology 36, 107-113.
17. Molina, J.M., Goguel, J., Sarfati, C., Michiels, J.F., Desportes-Livage, I., Balkan, S., Chastang, C., Cotte, L., Maslo, C., Struxiano, A., Derouin, F. and Decazes, JM. (2000) Trial of oral fumagillin for the treatment of intestinal microsporidiosis in patients with HIV infection. ANRS 054 Study Group. Agence Nationale de Recherche sur le SIDA. AIDS 14, 1341-1348.
18. Sparfel, J.-M., Sarfati, C., Liguory, O., Caroff, B., Dumoutier, N., Gueglio, B., Billaud, E., Raffi, F., Molina, J.-M., Miégeville, M. and Derouin, F. (1997) Detection of microsporidia and identification of Enterocytozoon bieneusi in surface water by filtration followed by PCR. Journal of Eukaryotic Microbiology 44, 78S.
19. Van Gool, T., Snijders, F., Reiss, P., Eefting Schattenkrek, J.K., der Bergh-Weerman, M.A., Bartelsman, J.F.W.M., Bruins, J.J.M., Canning, E.U. and Dankert, J. (1993) Diagnosis of intestinal and disseminated microsporidia infections in patients with HIV by a new rapid fluorescence technique. Journal of Clinical Pathology 46, 694-699.
20. Weber R., Bryan R.T., Schwartz D.A. and Owen R.L. (1994) Human microsporidial infections. Clinical Microbiological Review 7, 426-461.
21. Wittner, M.H., Tanowitz, B. and Weiss, L.M. (1993) Parasitic infections in AIDS patients. Parasitological Disease 7, 569-586.