Sensitive PCR diagnosis of infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA

Journal of Clinical Microbiology

Volumn 34, Issue 4, 1996, Pages 986-987

  Da Silva, Alexandre J ^a,d   Schwartz, David A ^b   Visvesvara, Govinda S ^a   De Moura, Hercules ^c   Slemenda, Susan B ^a   Pieniazek, Norman J ^a  

^a NATL CENTER FOR INFECTIOUS DISEASES   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^d CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME RNA;

AIDS RELATED COMPLEX; ARTICLE; DIAGNOSTIC ACCURACY; MICROSPORA; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTOZOAL INFECTION;

EID: 0029876589     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.4.986-987.1996     Document Type: Article

References (10)

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2. Fedorko, D. P., N. A. Nelson, and C. P. Cartwright. 1995. Identification of microsporidia in stool specimens by using PCR and restriction endonucleases J Clin. Microbiol. 33:1739-1741.
3. Franzen, C., A. Müller, P. Hegener, B. Salzberger, P. Hartmann, G. Fätkenheuer, V. Dichl, and M. Schrappe. 1995. Detection of microsporidia (Enterocytozoon bieneusi) in intestinal biopsy specimens from human immunodeficiency virus-infected patients by PCR. J. Clin. Microbiol. 33:2294-2296.
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6. van Gool, T., W. S. Hollister, J. Eeftinck Schattenkerk, M. A. Van den Bergh Weerman, W. J. Terpstra, R. J. van Ketel, P. Reiss, and E. U. Canning. 1990. Diagnosis of Enterocytozoon bieneusi microsporidiosis in AIDS patients by recovery of spores from faeces. Lancet 336:697-698.
7. Visvesvara, G. S., G. J. Leitch, N. J. Pieniazek, A. J. da Silva, S. Wallace, S. B. Slemenda, R. Weber, D. A. Schwartz, L. Gorelkin, C. M. Wilcox, and R. T. Bryan. 1995 Short-term in vitro culture and molecular analysis of the microsporidian, Enterocytozoon bieneusi. J Eukaryot. Microbiol. 42:506-510.
8. Weber, R., R. T. Bryan, R. L. Owen, C. M. Wilcox, L. Gorelkin, and G. S. Visvesvara. 1992. Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates N. Engl. J. Med 326:161-166
9. Weber, R., R. T. Bryan, D. A. Schwartz, and R. L. Owen. 1994. Human microsporidial infections Clin. Microbiol. Rev 7:426-461.
10. Zhu, X., M. Wittner, H. B. Tanowitz, D. Kotler, A. Call, and L. M. Weiss. 1993. Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use ot the polymerase chain reaction J. Infect. Dis. 168:1570-1575.