Xylazine, an α 2 -adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitro

Methods and Findings in Experimental and Clinical Pharmacology

Volumn 25, Issue 1, 2003, Pages 5-10

  Cupic V ^a   Čolić, Miodrag ^b   Jandric D ^b   Milojkovic B ^b   Varagic, V M ^c  

^a UNIVERSITY OF BELGRADE   (Serbia)

^b UNIVERSITY OF DEFENCE   (Serbia)

^c UNIVERSITY OF BELGRADE   (Serbia)

Author keywords

Apoptosis; Signaling; T cell hybridoma; Thymocytes; 2 Adrenergic agonist

Indexed keywords

ANTIBODY; BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; CYCLOSPORIN; DNA FRAGMENT; FAS ANTIBODY; FAS LIGAND ANTIBODY; MEROCYANINE; PROPIDIUM IODIDE; T LYMPHOCYTE RECEPTOR; UNCLASSIFIED DRUG; XYLAZINE; YOHIMBINE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL ACTIVATION; CELL DEATH; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CROSS LINKING; DRUG EFFECT; DRUG MECHANISM; IN VITRO STUDY; MALE; MOUSE; NONHUMAN; RAT; THYMOCYTE;

EID: 0037223624     PISSN: 03790355     EISSN: None     Source Type: Journal    
DOI: 10.1358/mf.2003.25.1.772541     Document Type: Article

References (25)

1. Felten, D.L., Felten, S.Y., Bellinger, D.L. et al. Noradrenergic sympathetic neural interactions with the immune system: structure and function. Immunol Rev 1987, 100: 225-60.
2. Jetschmann, J.U., Benschop, R.J., Jacobs, R. et al. Expression and in-vivo modulation of α- and β-adrenoceptors on human natural killer (CD16+) cells. J Neuroimmunol 1997, 74: 159-64.
3. 2 -adrenergic receptor: A mechanism for selective modulation of T helper 1 cell cytokine production. J Immunol 1997, 159: 4857-867.
4. Bubien, J.K., Cornwell, T., Bradfor, A.L., Fuller, C.M., Du Vall, M.D., Benos, D.J. α-Adrenergic receptors regulate human lymphocyte amiloride-sensitive sodium channels. Am J Physiol 1998, 275: 702-10.
5. Nelson, C.J., Lysle, D.T. Severity, time, and β-adrenergic receptor involvement in surgery-induced immune alterations. J Surg Res 1998, 80: 115-22.
6. Ellis, J.E., Pedlow, S., Bains, J. Premedication with clonidine does not attenuate suppression of certain lymphocyte subsets after surgery. Anesth Analg 1998, 87: 1426-30.
7. Lockwood, L.L., Silbert, L.H., Laudenslager, M.L., Watkins, L.R., Maier, S.F. Anesthesia-induced modulation of in vivo antibody levels: A study of pentobarbital, chloral hydrate, methoxyflurane, halothane, and ketamine/xylazine. Anesth Analg 1993, 77: 769-74.
8. 2 -receptors. J Neuroimmunol 1995, 57: 27-34.
9. 2 adrenergic agonist, on rat spleen cells in culture. J Neuroimmunol 2001, 113: 19-29.
10. 2 -adrenergic agonist, modulates proliferaton of rat thymocytes in vivo and in vitro. Methods Find Exp Clin Pharmacol 2000, 22(7): 557-62.
11. Brander, G.C., Pugh, D.M., Bywater, R.J., Jenkins, W.L. Veterinary Applied Pharmacology and Therapeutics. Bailliere Tindal: London 1994.
12. Howard, H.L., McLauglin-Taylor, E., Hill, R.L. The effect of mouse euthanasia technique on subsequent lymphocyte proliferation and cell mediated lympholysis assays. Lab Anim Sci 1990, 40: 510-14.
13. Correa-Sales, C., Tosta, C.E., Rizzo, L.V. The effect of anesthesia with thiopental on T lymphocyte responses to antigen and mitogens in vivo and in vitro. Int J Immunopharmacol 1997, 117-28.
14. Gummert, J.F., Otto, G., Barten, M.J., Morris, R.E. Effect of anesthesia on a whole blood lymphocyte proliferation assay in the rat. Immunopharmacol Immunotoxicol 1999, 21: 267-76.
15. Popović, L., Čolić, M., Kosec D. Production and characterization of the rat thymic T-cell hybridomas. Vojnosanit Pregl 1994, 51: 56-9.
16. Popović, P., Čolić, M., Vuċević, D., Gašić, S., Pavićić, L. Comparison of signaling pathways involved in apoptosis of a thymocyte hybridoma triggered by a rat thymic medullary epithelial cell line, dexamethasone or T-cell receptor cross-linking. Immunol Lett 2000, 72: 83-91.
17. Mower, D.A., Peckham, D.W., Illera, V.A., Fishbaugh, J.K., Stuhz, L.L., Ashman, R.F. Decreased membrane phospholipid packing and decreased cell size precede DNA clevage in murine mouse B cell apoptosis. J Immunol 1994, 152: 4832-42.
18. Bergquist, J., Josefsson, E., Tarkowski A., Ekman, R., Ewing, A. Measurements of catecholamine-mediated apoptosis of immunocompetent cells by capilary electrophoresis. Electrophoresis 1997, 18: 1760-6.
19. Haberfeld, M., Johnson, R.Q., Ruben, L.N., Clothier, R.H., Shiigi, S. Adrenergic modulation of apoptosis in splenocytes of Xenopus laevis in vitro. Neuroimmunomodulation 1999, 6: 175-81.
20. Street, J.A., Web, J.G., Bright, P.S., Gaffney, T.E. Accumulation, subcelluar localization and release of propranolol from synaptosomes of rat cerebral cortex. J Pharmacol Exp Ther 1984, 229: 154-61.
21. Zacharchuk, C.M., Mercep, M., Chakraborti, P.K., Simons, S.S., Jr., Ashwell, J.D. Programmed T lymphocyte death. Cell activation- and steroid-induced pathways are mutually antagnoistic. J Immunol 1990, 145(12): 403-45.
22. Mower, D.A., Peckham, D.W., Illera, V.A., Fishbaugh, J.K., Stuhz, L.L., Ashman, R.F. Decreased membrane phospholipid packing and decreased cell size precede DNA clevage in murine mouse B cell apoptosis. J Immunol 1994, 152: 4832-42.
23. Yazdanbakhsh, K., Choi, J.W., Li, Y., Lau, L.F., Choi, Y. Cyclosporine blocks apoptosis by inhibiting the DNA binding activity of the transcription factor Nur77. Proc Natl Acad Sci USA 1995, 92(2): 437-41.
24. Scaffidi, C., Kirchhoff, S., Krammer, P.H., Peter, M.E. Apoptosis signaling in lymphocites. Curr Opin Immunol 1999, 11: 277-85.
25. Denis, F., Rheaume, E., Aouad, S.M., Alam, A., Sekaly, R.P., Cohen, L. The role of caspases in T cell development and the control of immune responses. Cell Mol Life Sci 1998, 54: 1005-19.