Identification of a novel insertion sequence in vanB2-containing Enterococcus faecium

Letters in Applied Microbiology

Volumn 36, Issue 3, 2003, Pages 186-190

  Lee, Wee Gyo ^a,b   Kim, W ^a  

^a Ajou University School of Medicine   (South Korea)

^b Ajou University Medical Center   (South Korea)

Author keywords

Enterococcus faecium; Insertion sequence; IS3 family; Transposase; vanB2

Indexed keywords

BACILLI;

ENTEROCOCCUS FAECIUM; EPIDEMIOLOGICAL STUDIES; INSERTION SEQUENCES; IS3 FAMILY; OPEN READING FRAME; PCR AMPLIFICATION; SEQUENCE ANALYSIS; TRANSPOSASE; VANB2;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA;

BACTERIUM;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA EXTRACTION; ENTEROCOCCUS FAECIUM; EPIDEMIOLOGY; GENE AMPLIFICATION; GENE INSERTION; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; ENTEROCOCCUS FAECIUM; INSERTION SEQUENCES; POSIBACTERIA; UNIDENTIFIED INSERTION SEQUENCE;

EID: 0037223424     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.2003.01292.x     Document Type: Article

References (14)

1. Carias, L.L., Rudin, S.D., Donskey, C.J. and Rice, L.B. (1998) Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate. Journal of Bacteriology 180, 4426-4434.
2. Dahl, K.H., Lundblad, E.W., Røkenes, T.P., Olsvik, Ø. and Sundsfjord, A. (2000) Genetic linkage of the vanB2 gene cluster to Tn5382 in vancomycin-resistant enterococci and characterization of two novel insertion sequences. Micorbiology 146, 1469-1479.
3. Fischetti, V.A., Novick, R.P., Ferretti, J.J., Portnoy, D.A. and Rood, J.I. (2000) Gram-Positive Pathogens. Washington, DC: American Society for Microbiology.
4. Garnier, F., Taourit, S., Glaser, P., Courvalin, P. and Galimand, M. (2000) Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp. Microbiology 146, 1481-1489.
5. Haaheim, H., Dahl, K.E., Simonsen, G.S., Olsvik, Ø. and Sundstjord, A. (1998) Long PCRs of transposons in the structural analysis of genes encoding acquired glycopeptide resistance in enterococci. BioTechniques 24, 432-437.
6. Huang, G., Zhang, L. and Birch R.G. (2000) Rapid amplification and cloning of Tn5 flanking fragments by inverse PCR. Applied Microbiology 31, 149-153.
7. Lee, W. G., Jernigan, J.A., Racheed, J.K., Anderson, G.J. and Tenover, F.C. (2001) Possible horizontal transfer of the vanB2 gene among genetically diverse strains of vancomycin-resistant Enterococcus faecium in a Korean hospital. Journal of Clinical Microbiology 39, 1165-1168.
8. Mahillon, J. and Chandler, M. (1998) Insertion sequences. Microbiology and Molecular Biology Reviews 62, 725-774.
9. McGregor, K.F., Nolan, C., Young, H.K., Palepou, M.F., Tysall, L. and Woodford, N. (2001) Prevalence of the vanB2 gene cluster in VanB glycopeptide-resistant enterococci in the United Kingdom and the Republic of Ireland and its association with a Tn5382-like element. Antimicrobial Agents and Chemotherapy 45, 367-368.
10. Patel, R., Uhl, J.R., Kohner, P., Hopkins, M.K., Steckelberg, J.M., Kline, B. and Cockerill III, F.R. (1998) DNA sequence variation within vanA, vanB, vanC-1, and vanC2/3 genes of clinical Enterococcus isolates. Antimicrobial Agents and Chemotherapy 42, 202-205.
11. Pitcher, D.G., Saunders, N.A. and Owen, R.J. (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Letters in Applied Microbiology 8, 151-156.
12. Quintiliani Jr, R. and Courvalin, P. (1996) Characterization of Tn1547, a composite transposon flanked by the IS16 and IS256-like elements, that confers vancomycin resistance in Enterococcus faecalis BM428. Gene 172, 1-8.
13. Trieu-Cuot, P., Carlier, D. and Couvalin, P. (1988) Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli. Journal of Bacteriology 170, 4388-4391.
14. Woodford, N., McNamara, E., Smyth, E. and George, R.C. (1992) High-level resistance to gentamicin in Enterococcus faecium. Journal of Antimicrobial Chemotherpy 29, 395-403.