Long PCRs of transposon in the structural analysis of genes encoding acquired glycopeptide resistance in enterococci

BioTechniques

Volumn 24, Issue 3, 1998, Pages 432-437

  Haaheim, H ^a   Dahl, K H ^a   Simonsen, G S ^a,b   Olsvik, O ^a,b   Sundsfjord, A ^a,b  

^a UNIVERSITY OF TROMSØ   (Norway)

^b UNIVERSITY HOSPITAL OF NORTH NORWAY   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; GLYCOPEPTIDE;

ANTIBIOTIC RESISTANCE; ARTICLE; ENTEROCOCCUS; GENE CLUSTER; GENE STRUCTURE; GENETIC HETEROGENEITY; NONHUMAN; POLYMERASE CHAIN REACTION; TRANSPOSON; AGAR GEL ELECTROPHORESIS; BACTERIAL GENE; DRUG EFFECT; GENETICS; HUMAN; METHODOLOGY;

ANIMALIA; BACTERIA (MICROORGANISMS); ENTEROCOCCUS; PROKARYOTA;

DNA TRANSPOSABLE ELEMENTS; DRUG RESISTANCE, MICROBIAL; ELECTROPHORESIS, AGAR GEL; ENTEROCOCCUS; GENES, BACTERIAL; GLYCOPEPTIDES; HUMANS; POLYMERASE CHAIN REACTION;

EID: 0031594570     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98243st02     Document Type: Article

References (20)

1. Aarestrup, F. 1995. Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms. Microb. Drug Res. 1:255-257.
2. Arthur, M., C. Molinas, F. Depardieu and P. Courvalin. 1993. Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147. J. Bacteriol. 175:117-127.
3. Bates, J., J.Z. Jordens and D.T. Griffiths. 1994. Farm animals as a putative reservoir for vancomycin-resisiant enterococcal infection in man. J. Antimicrob. Chemother. 34:507-516.
4. Breslauer, K.J., R. Frank, H. Blocker and L.A. Markey. 1986. Predicting DNA duplex stability from the base sequence. Proc. Natl. Acad. Sci. USA 83:3746-3750.
5. Chadwick, P.R., N. Woodford, E.B. Kaczmarski, S. Gray, R.A. Barrell and B.A. Oppenheim. 1996. Glycopeptide-resistant enterococci isolated from uncooked meat. J. Antimicrob. Chemother. 38:908-909.
6. Dunne, W.M., Jr., B.S. Dunne and D. Smith. 1996. Watch out where the huskies go. ASM News 62:283.
7. B two-component regulatory system in Enterococcus faecalis V583. J. Bacteriol. 178:1302-1309.
8. Foord, O.S. and E.A. Rose. 1994. Long-distance PCR. PCR Methods Appl. 3:S149-S161.
9. Griffais, R., P.M. Andre and M. Thibon. 1991. K-tuple frequency in the human genome and polymerase chain reaction. Nucleic Acids Res. 19:3887-3891.
10. Handwerger, S., J. Skoble, L.F. Discotto and M.J. Pucci. 1995. Heterogeneity of the vanA gene cluster in clinical isolates of enterococci from the Northeastern United States. Antimicrob. Agents Chemother. 39:362-368.
11. Klare, I., H. Heier, H. Claus, G. Bohme, S. Marin, G. Seltmann, R. Hakenbeck, V. Antanassova and W. Witte. 1995. Enterococcus faecium strains with vanA-mediated high-level glycopeptide resistance isolated from animal foodstuffs and fecal samples of humans in the community. Microb. Drug Res. 1:265-272.
12. Kwok, S. 1990. Procedures to minimize PCR-product carry-over, p. 142-145. In M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (Eds.), PCR Protocols. A Guide to Methods and Applications, Academic Press, San Diego.
13. Leclerq, R. 1997. Enterococci acquire new kinds of resistance. Clin. Infect. Dis. 24 (Suppl 1):S80-S84.
14. Quintiliani, R., Jr. and P. Courvalin. 1996. Characterization of Tn1547, a composite transposon flanked by the IS16 and IS256-like elements, that confers vancomycin resistance in Enterococcus faecalis BM4281. Gene 172:1-8.
15. Sader, H.S., M.A. Pfaller, F.C. Tenover, R.J. Hollis and R.N. Jones. 1994. Evaluation and characterization of multiresistant Enterococcus faecium from 12 U.S. medical centers. J. Clin. Microbiol. 32:2840-2842.
16. Sahm, D., J. Kissinger, M.S. Gilmore, P.R. Murray, R. Mulder, J. Solliday and B. Clarke. 1989. In vitro susceptibility studies of vancomycin-resistant Enterococcus faecalis. Antimicrob. Agents Chemother. 33:1588-1591.
17. Van Belkum, A., N. van den Braak, R. Thomassen, H. Verburgh and H. Endtz. 1996. Vancomycin-resistant enterococci in cats and dogs. Lancet 348:1038-1039.
18. Van der Auwera, P., N. Pensart, V. Korten, B.E. Murray and R. Leclerq. 1996. Influence of oral glycopeptides on the fecat flora of human volunteers:selection of highly glycopeptide-resistant enterococci. J. Infect. Dis. 173:1129-1136.
19. Willey, B.M., A.J. McGeer, M.A. Ostrowski, B.N. Kreiswirth and D.E. Low. 1994. The use of molecular typing techniques in the epidemiologic investigation of resistant enterococci. Infect. Control Hosp. Epidemiol. 15:549-556.
20. Woodford, N., A.P. Watson and P.R. Chadwick. 1995. Investigation by long PCR (L-PCR) of the genetic elements mediating VanA glycopeptide resistance in enterococci from uncooked meat in South Manchester. In Abstracts of the XIIIth Lancefield International Symposium on Streptococci and Streptococcal Diseases, Abstract 285 bis. Paris, France.