The yeast two-hybrid system for identifying protein-protein interactions

Journal of Pathology

Volumn 199, Issue 1, 2003, Pages 4-7

  Coates, P J ^a   Hall, P A ^b  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Protein interactions; Transcription factor; Two hybrid assay; Yeast genetics

Indexed keywords

ARTICLE; CELL ACTIVITY; HUMAN; MOLECULAR SIZE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; TWO HYBRID SYSTEM;

ANIMALS; DNA; DNA, CIRCULAR; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; PROTEINS; RNA; RNA, MESSENGER; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC; TWO-HYBRID SYSTEM TECHNIQUES; YEASTS;

EID: 0037218918     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/path.1267     Document Type: Article

References (27)

1. Oliver S. Guilt-by-association goes global. Nature 2000; 403: 601-603.
2. Schmidt MHH, Broll R, Bruch H-P, Bogler O, Duchrow M. The proliferation marker pKi-67 organizes the nucleolus during the cell cycle depending on Ran and cyclin B. J Pathol 2003; 199: 18-27.
3. Fields S, Song O. A novel genetic system to detect protein-protein interactions. Nature 1989; 340: 245-247.
4. Chien CT, Bartel PL, Sternglanz R, Fields S. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci U S A 1991; 88: 9578-9582.
5. Gyuris J, Golemis E, Chertkov H, Brent R. Cdi. a human G1 and S-phase protein phosphatase that associates with Cdk2. Cell 1993; 75: 791-803.
6. Estojak J, Brent R, Golemis EA. Correlation of two-hybrid affinity data with in vitro measurements. Mol Cell Biol 1995; 15: 5820-5829.
7. Iwabuchi K, Li B, Bartel P, Fields S. Use of the two-hybrid system to identify the domain of p53 involved in oligomerization. Oncogene 1993; 8: 1692-1696.
8. Hall PA, Kearsey JM, Coates PJ, Norman DG, Warbrick E, Cox LS. Characterisation of the interaction between PCNA and Gadd45. Oncogene 1995; 10: 2427-2433.
9. Li JJ, Herskowitz I. Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. Science 1993; 262: 1870-1874.
10. Alexander MK, Bourns BD, Zakian VA. One-hybrid systems for detecting protein-DNA interactions. Methods Mol Biol 2001; 177: 241-259.
11. Tirode F, Malaguti C, Romero F, Attar R, Camonis J, Egly JM. A conditionally expressed third partner stabilizes or prevents the formation of a transcriptional activator in a three-hybrid system. J Biol Chem 1997; 272: 22995-22999.
12. Putz U, Skehel P, Kuhl D. A tri-hybrid system for the analysis and detection of RNA-protein interactions. Nucleic Acids Res 1996; 24: 4838-4840.
13. Licitra EJ, Liu JO. A three-hybrid system for detecting small ligand-protein receptor interactions. Proc Natl Acad Sci U S A 1996; 93: 12817-12821.
14. Vidal M, Brachmann RK, Fattaey A, Harlow E, Boeke JD. Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proc Natl Acad Sci U S A 1996; 93: 10315-10320.
15. Vidal M, Legrain P. Yeast forward and reverse 'n'-hybrid systems. Nucleic Acids Res 1999; 27: 919-929.
16. Bartel P, Chien C-T, Sternglanz R, Fields S. Elimination of false positives that arise in using the two-hybrid system. Biotechniques 1993; 14: 920-924.
17. Hengen PN. False positives from the yeast two-hybrid system. Trends Biochern Sci 1997; 22: 33-34.
18. Warbrick E. Two's company, three's a crowd: the yeast two hybrid system for mapping molecular interactions. Structure 1997; 5: 13-17.
19. Golemis EA, Serebriski I, Law SF. The yeast two-hybrid system: criteria for detecting physiologically significant protein-protein interactions. Curr Issues Mol Biol 1999; 1: 31-45.
20. Serebriski I, Estojak J, Berman M, Golemis EA. Approaches to detecting false positives in yeast two-hybrid systems. Biotechniques 2000; 28: 332-336.
21. Luo Y, Bataolo A, Zhou H, Zhu L. Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system. Biotechniques 1997; 22: 350-352.
22. Uetz P, Giot L, Cagney G, et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature 2000; 403: 623-627.
23. Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci U S A 2001; 98: 4569-4574.
24. Kim SK. Http://C.elegans: mining the functional genomic landscape. Nature Rev Genet 2001; 2: 681-689.
25. Tucker CL, Gera JF, Uetz P. Towards an understanding of complex protein networks. Trends Cell Biol 2001; 11: 102-106.
26. von Meering C, Krause R, Snel B, et al. Comparative assessment of large-scale data sets of protein-protein interactions. Nature, 2002; 417: 399-403.
27. Xenarios I, Fernandez E, Salwinski L, et al. DIP: the database of interacting proteins: 2001 update. Nucleic Acids Res 2001; 29: 239-241.