Characterisation of the genes encoding Aa1 isoforms from the scorpion Androctonus australis

Toxicon

Volumn 41, Issue 1, 2003, Pages 115-119

  Legros, Christian ^a,b   Bougis, Pierre E ^a   Martin Eauclaire, Marie France ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

^b UNIVERSITÉ D EVRY VAL D ESSONNE   (France)

Author keywords

cDNA; Gene; Potassium channel; Scorpion toxin

Indexed keywords

COMPLEMENTARY DNA; ISOPROTEIN; PROTEIN AATX1; PROTEIN AATX2; PROTEIN PRECURSOR; SCORPION VENOM; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID COMPOSITION; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; EXON; GENETIC ANALYSIS; GENETIC CODE; INTRON; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCORPION; SEQUENCE HOMOLOGY;

ANIMALS; BASE SEQUENCE; DNA PRIMERS; ISOMERISM; MOLECULAR SEQUENCE DATA; NEUROTOXINS; POLYMERASE CHAIN REACTION; SCORPION VENOMS; SCORPIONS;

ANDROCTONUS AUSTRALIS; SCORPIONES;

EID: 0037213243     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(02)00212-X     Document Type: Article

References (22)

1. Aiyar J., Withka J.M., Rizzi J.P., Singleton D.H., Andrews G.C., Lin W., Boyd J., Hanson D.C., Simon M., Dethlefs B., Lee C.L., Hall J.E., Gutman G.A., Chardy K.G. Topology of the pore-region of a K+ channel revealed by the NMR-derived structures of scorpion toxins. Neuron. 15:1995;1169-1181.
2. Becerril B., Corona M., Mejia M.C., Martin B.M., Lucas S., Bolivar F., Possani L.D. The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron. FEBS Lett. 335:1993;6-8.
3. 1H-NMR. Charybdotoxin possesses a structural motif found in other scorpion toxins. Eur. J. Biochem. 196:1991;19-28.
4. Bougis P.E., Rochat H., Smith L.A. Precursors of Androctonus australis scorpion neurotoxins. Structures of precursors, processing outcomes, and expression of a functional recombinant toxin II. J. Biol. Chem. 264:1989;19259-19265.
5. Breathnach R., Chambon P. Organization and expression of eukaryotic split genes coding for proteins. Annu. Rev. Biochem. 50:1981;349-383.
6. Ceard B., Martin-Eauclaire M., Bougis P.E. Evidence for a position-specific deletion as an evolutionary link between long- and short-chain scorpion toxins. FEBS Lett. 494:2001;246-248.
7. Corona M., Zurita M., Possani L.D., Becerril B. Cloning and characterization of the genomic region encoding toxin IV-5 from the scorpion Tityus serrulatus Lutz and Mello. Toxicon. 34:1996;251-256.
8. Delabre M.L., Pasero P., Marilley M., Bougis P.E. Promoter structure and intron-exon organization of a scorpion alpha-toxin gene. Biochemistry. 34:1995;6729-6736.
9. Froy O., Sagiv T., Poreh M., Urbach D., Zilberberg N., Gurevitz M. Dynamic diversification from a putative common ancestor of scorpion toxins affecting sodium, potassium, and chloride channels. J. Mol. Evol. 48:1999;187-196.
10. Gmachl M., Kreil G. The precursors of the bee venom constituents apamin and MCD peptide are encoded by two genes in tandem which share the same 3′-exon. J. Biol. Chem. 270:1995;12704-12708.
11. John T.R., Smith L.A., Kaiser I.I. Genomic sequences encoding the acidic and basic subunits of Mojave toxin: unusually high sequence identity of non-coding regions. Gene. 139:1994;229-234.
12. Laraba-Djebari F., Legros C., Crest M., Ceard B., Romi R., Mansuelle P., Jacquet G., van Rietschoten J., Gola M., Rochat H., Bougis P.E., Martin-Eauclaire M.-F. The kaliotoxin family enlarged. Purification, characterization, and precursor nucleotide sequence of KTX2 from Androctonus australis venom. J. Biol. Chem. 269:1994;32835-32843.
13. Legros C., Pollmann V., Knaus H.G., Farrell A.M., Darbon H., Bougis P.E., Martin-Eauclaire M.F., Pongs O. Generating a high affinity scorpion toxin receptor in KcsA-Kv1.3 chimeric potassium channels. J. Biol. Chem. 275:2000;16918-16924.
14. Martin M.F., Rochat H., Marchot P., Bougis P.E. Use of high performance liquid chromatography to demonstrate quantitative variation in components of venom from the scorpion Androctonus australis Hector. Toxicon. 25:1987;569-573.
15. + channel. Biochemistry. 35:1996;6181-6187.
16. Nakashima K., Ogawa T., Oda N., Hattori M., Sakaki Y., Kihara H., Ohno M. Accelerated evolution of Trimeresurus flavoviridis venom gland phospholipase A2 isozymes. Proc. Natl Acad. Sci. USA. 90:1993;5964-5968.
17. Pisciotta M., Coronas F.I., Bloch C., Prestipino G., Possani L.D. Fast K(+) currents from cerebellum granular cells are completely blocked by a peptide purified from Androctonus australis Garzoni scorpion venom. Biochim. Biophys. Acta. 1468:2000;203-212.
18. + channel selectivity filter by mutant cycle-based structure analysis. Neuron. 16:1996;131-139.
19. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
20. Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA. 74:1977;5463-5467.
21. Tytgat J., Chandy K.G., Garcia M.L., Gutman G.A., Martin-Eauclaire M.F., van der Walt J.J., Possani L.D. A unified nomenclature for short-chain peptides isolated from scorpion venoms: alpha-KTx molecular subfamilies. Trends Pharmacol. Sci. 20:1999;444-447.
22. + channel: pharmacological characterization and localization in rat brain. FEBS Lett. 501:2001;31-36.