Human neutrophils isolated from peripheral blood contain Ku protein but not DNA-dependent protein kinase

International Journal of Biochemistry and Cell Biology

Volumn 35, Issue 1, 2003, Pages 86-94

  Kurosawa, Aya ^a,b   Shinohara, Ken Ichi ^a   Watanabe, Fumiaki ^a   Shimizu Saito, Keiko ^a   Koiwai, Osamu ^b   Yamamoto, Kohtaro ^a   Teraoka, Hirobumi ^a  

^a Tokyo Medical and Dental University ^*  (Japan)

^b TOKYO UNIVERSITY OF SCIENCE   (Japan)

Author keywords

DNA dependent protein kinase; Ku protein; Neutrophil; Non homologous end joining; Protease inhibitor

Indexed keywords

DNA DEPENDENT PROTEIN KINASE; ISOENZYME; ISOPROTEIN; KU ANTIGEN; MESSENGER RNA; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PROTEIN; PROTEIN P470; PROTEINASE INHIBITOR; UNCLASSIFIED DRUG; CELL NUCLEUS ANTIGEN; DNA BINDING PROTEIN; HELICASE; NUCLEAR PROTEIN; PRKDC PROTEIN, HUMAN; PROTEIN SERINE THREONINE KINASE; XRCC5 PROTEIN, HUMAN;

ARTICLE; CELL ISOLATION; CELL NUCLEUS; CONTROLLED STUDY; DNA METABOLISM; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; NEUTROPHIL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; CHEMISTRY; DIMERIZATION; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY;

ANTIGENS, NUCLEAR; CELL NUCLEUS; DIMERIZATION; DNA HELICASES; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; FLOW CYTOMETRY; GENE EXPRESSION; HUMANS; NEUTROPHILS; NUCLEAR PROTEINS; POLY(ADP-RIBOSE) POLYMERASES; PROTEASE INHIBITORS; PROTEIN-SERINE-THREONINE KINASES;

EID: 0037212682     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1357-2725(02)00123-1     Document Type: Article

References (33)

1. Mimori T., Akizuki H., Yamagata S., Inada S., Yoshida S., Homma M. Characterization of a high-molecular-weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap. J. Clin. Invest. 68:1981;611-620.
2. Mimori T., Hardin J.A. Mechanism of interaction between Ku protein and DNA. J. Biol. Chem. 261:1986;10375-10379.
3. Dvir A., Peterson S.R., Knuth M.W., Lu H., Dynan W.S. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II. Proc. Natl. Acad. Sci. U.S.A. 89:1992;11920-11924.
4. Gottilieb T.M., Jackson S.P. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell. 72:1993;131-142.
5. Watanabe F., Teraoka H., Iijima S., Mimori T., Tsukada K. Molecular properties, substrate specificity and regulation of DNA-dependent protein kinase from Raji Burkitt's lymphoma cells. Biochim. Biophys. Acta. 1223:1994;255-260.
6. Hartley K.O., Gell D., Smith G.C., Zhang H., Divecha N., Connelly M.W., Admon A., Lees-Miller S.P., Anderson C.W., Jackson S.P. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell. 82:1995;849-856.
7. Taccioli G.E., Gottlieb T.M., Blunt T., Priestly A., Demengeot J., Mizuta R., Lehmann A.R., Alt F.W., Jackson S.P., Jeggo P.A. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science. 265:1994;1442-1445.
8. Peterson S.R., Kurimasa A., Shimura M., Dynan W.S., Bradbury E.M., Chen D.J. Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand break repair mutant mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 92:1995;3171-3174.
9. Blunt T., Finnie N.J., Taccioli G.E., Smith G.C.M., Demengeot J., Gottlieb T.M., Mizuta R., Varghese A.J., Alt F.W., Jeggo P.A. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine SCID mutation. Cell. 80:1995;813-823.
10. Nagasawa M., Watanabe F., Suwa A., Yamamoto K., Tsukada K., Teraoka H. Nuclear translocation of the catalytic component of DNA-dependent protein kinase upon growth stimulation in normal human T lymphocytes. Cell Struct. Funct. 22:1997;585-594.
11. Morio T., Hanissian S.H., Bacharier L.B., Teraoka H., Nonoyama S., Seki M., Kondo J., Nakano H., Lee S.H., Geha R.S., Yata J. Ku in cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb. Immunity. 11:1999;339-348.
12. Ajmani A.K., Satoh M., Reap E., Cohen P.L., Reeves W.H. Absence of autoantigen Ku in mature human neutrophils and human promyelocytic leukemia line (HL-60) cells and lymphocytes undergoing apoptosis. J. Exp. Med. 181:1995;2049-2058.
13. Bhatia M., Kirkland J.B., Meckling-Gill K.A. Modulation of poly(ADP-ribose) polymerase during neutrophilic and monocyte differentiation of promyelocytic (NB4) and myelocytic (HL-60) leukaemia cells. Biochem. J. 308:1995;131-137.
14. Teraoka H., Mikoshiba M., Takase K., Yamamoto K., Tsukada K. Reversible G1 arrest induced by dimethyl sulfoxide in human lymphoid cell lines: dimethyl sulfoxide inhibits IL-6-induced differentiation of SKW6-CL4 into IgM-secreting plasma cells. Exp. Cell. Res. 222:1996;218-224.
15. Trucco C., Oliver F.J., Murcia G.D., Murcia J.M. DNA repair defect in poly(ADP-ribose) polymerase-deficient cell lines. Nucleic Acids Res. 26:1998;2644-2649.
16. Schwartz J.L., Gaulden M.E. The relative contributions of B and T lymphocytes in the human peripheral blood mutagen test system as determined by cell survival, mitogenic stimulation, and induction of chromosome aberrations by radiation. Environ. Mutagen. 2:1980;473-485.
17. Terai C., Wasson D.B., Carrera C.J., Carson D.A. Dependence of cell survival on DNA repair in human mononuclear phagocytes. J. Immonol. 147:1991;4302-4306.
18. Masutani M., Nozaki T., Wakabayashi K., Sugimura T. Role of poly(ADP-ribose) polymerase in cell-cycle checkpoint mechanisms following γ-irradiation. Biochimie. 77:1995;462-465.
19. Tewari M., Quan L.T., O'Rourke K., Desnoyers S., Zeng Z., Beidler D.R., Poirier G.G., Salvesen G.S., Dixit V.M. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell. 81:1995;801-809.
20. 3H ] thymidine into human haemopoietic cell lines Int. J. Radiat. Biol. 76:2000;295-303.
21. Teraoka H., Yumoto Y., Watanabe F., Tsukada K., Suwa A., Enari M., Nagata S. CPP32/Yama/apopain cleaves the catalytic component of DNA-dependent protein kinase in the holoenzyme. FEBS Lett. 393:1996;1-6.
22. Lazebnik Y.A., Kaufmann S.H., Desnoyers S., Poirier G.G., Earnshaw W.C. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature. 371:1994;346-347.
23. Song Q., Lees-Miller S.P., Kumar S., Zhang N., Chan D.W., Smith G.C.M., Jackson S.P., Alnemri E.S., Litwack G., Khanna K.K., Lavin M.F. DNA-dependent protein kinase catalytic subunit: a target for an ICE-like protein in apoptosis. EMBO J. 15:1996;3238-3246.
24. Han Z., Malik K., Carter T., Reeves W.H., Whyche J.H., Hendrickson E.A. DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease. J. Biol. Chem. 271:1996;25035-25040.
25. Liles W.C., Kiener P.A., Ledbetter J.A., Aruffo A., Klebanoff S.J. Differential expression of Fas (CD95) and Fas ligand on normal human phagocytes: implications for the regulation of apoptosis in neutrophils. J. Exp. Med. 184:1996;429-440.
26. Watson R.W.G., Redmond H.P., Wang J.H., Condron C., Bouchier-Hayes D. Neutrophils undergo apoptosis following ingestion of Escherichia coli. J. Immunol. 156:1996;3986-3992.
27. Li G., Nelsen C., Hendrickson E.A. Ku86 is essential in human somatic cells. Proc. Natl. Acad. Sci. U.S.A. 91:2002;832-837.
28. Myung K., He D.M., Lee S.E., Hendrickson E.A. KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen locus is implicated in the control of DNA-dependent protein kinase activity. EMBO J. 16:1997;3172-3184.
29. Myung K., Braastad C., He D.M., Hendrickson E.A. KARP-1 is induced by DNA damage in a p53- and ataxia telangiectasia mutated-dependent fashion. Proc. Natl. Acad. Sci. U.S.A. 95:1998;7664-7669.
30. Tuteja N., Tuteja R., Ochem A., Taneja P., Huang N.W., Simoncsits A., Susic S., Rahman K., Marusic L., Chen J., Zhang J., Wang S., Pongor S., Falaschi A. Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen. EMBO J. 13:1994;4991-5001.
31. Giffin W., Torrance H., Rodda D.J., Prefontaine G.G., Pope L., Hache R.J. Sequence-specific DNA-binding by Ku autoantigen and its effects on transcription. Nature. 380:1996;265-268.
32. Roberts M.R., Han Y., Fienberg A., Hunihan L., Ruddle F.H. A DNA-binding activity, TRAC, specific for the TRA element of the transferring receptor gene copurifies with the Ku autoantigen. Proc. Natl. Acad. Sci. U.S.A. 91:1994;6354-6358.
33. Shore D. Telomeres: unsticky end. Science. 281:1998;1818-1819.