Applicability of tetrazolium salts for the measurement of respiratory activity and viability of groundwater bacteria

Journal of Microbiological Methods

Volumn 52, Issue 1, 2003, Pages 47-58

  Hatzinger, Paul B ^a   Palmer, Patrick ^b   Smith, Richard L ^c   Peñarrieta, Cecilia T ^c   Yoshinari, Tadashi ^b  

^a The Shaw Group Inc   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c DENVER FEDERAL CENTER   (United States)

Author keywords

Bacteria; CTC; ETS activity; Groundwater; INT; Tetrazolium salts; XTT

Indexed keywords

2 (4 IODOPHENYL) 3 (4 NITROPHENYL) 5 PHENYLTETRAZOLIUM; 5 CYANO 2,3 DITOLYLTETRAZOLIUM CHLORIDE; FORMAZAN; GROUND WATER; OXYGEN; SODIUM 3' [1 (PHENYLAMINO)CARBONYL] 3,4 TETRAZOLIUM BIS(4 METHOXY 6 NITRO)BENZENESULFONIC ACID; TETRAZOLIUM; UNCLASSIFIED DRUG;

AEROBIC METABOLISM; AQUIFER; ARTICLE; BACTERIAL COUNT; BACTERIAL STRAIN; BACTERICIDAL ACTIVITY; BACTERIUM; BACTERIUM CULTURE; BACTERIUM ISOLATE; CELL DENSITY; CELL VIABILITY; CONTROLLED STUDY; CRYSTAL; ELECTRON TRANSPORT; GAS CHROMATOGRAPHY; INCUBATION TIME; MICROBIAL ACTIVITY; MICROBIAL RESPIRATION; MICROSCOPY; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; SAND; TOXICITY;

BACTERIA, AEROBIC; ECOSYSTEM; INDICATORS AND REAGENTS; OXYGEN CONSUMPTION; TETRAZOLIUM SALTS; THIAZOLES;

BACTERIA (MICROORGANISMS); GADUS MORHUA; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0037208477     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00132-X     Document Type: Article

References (49)

1. Barbaro S.E., Albrechtsen H.J., Jensen B.K., Mayfield C.I., Barker J.F. Relationships between aquifer properties and microbial populations in the Borden Aquifer. Geomicrobiol. J. 12:1994;203-219.
2. Bensaid A., Thierie J., Penninckx M. The use of tetrazolium salt XTT for the estimation of biological activity of activated sludge cultivated under steady-state and transient regimes. J. Microbiol. Methods. 40:2000;255-263.
3. Bhupathiraju V.K., Hernandez M., Landfear D., Alvarez-Cohen L. Application of tetrazolium dyes as an indicator of viability in anaerobic bacteria. J. Microbiol. Methods. 37:1999;231-243.
4. Blenkinsopp S.A., Lock M.A. The measurement of electron transport system activity in river biofilms. Water Res. 24:1990;441-445.
5. Bone T.L., Balkwill D.L. Morphological and cultural comparison of microorganisms in surface soil and subsurface sediments at a pristine study site in Oklahoma. Microb. Ecol. 16:1988;49-64.
6. Braun-Howland E.B., Danielsen S.A., Nierzwicki-Bauer S.A. Development of a rapid method for detecting bacterial cells in situ using 16S rRNA-targeted probes. BioTechniques. 13:1992;928-933.
7. Casida L.E. Jr., Klein D.A., Somtoro T. Soil dehydrogenase activity. Soil Sci. 98:1964;371-376.
8. Christensen J.P., Owens T.G., Devol A.H., Packard T.T. Respiration and physiological state in marine bacteria. Mar. Biol. 55:1980;267-276.
9. Coallier J., Prevost M., Rompre A., Duchesne D. The optimization and application of two direct viable count methods for bacteria in distributed drinking water. Can. J. Microbiol. 40:1994;830-836.
10. del Giorgio P.A., Scarborough G. Increase in the proportion of metabolically active bacteria along gradients of enrichment in freshwater and marine plankton: implications for estimates of bacterial growth and production rates. J. Plankton Res. 17:1995;1905-1924.
11. Griebe T., Schaule G., Wuertz S. Determination of microbial respiratory and redox activity in activated sludge. J. Ind. Microbiol. Biotech. 19:1997;118-122.
12. Harvey R.W., George L. Growth determinations for unattached bacteria in a contaminated aquifer. Appl. Environ. Microbiol. 53:1987;2992-2996.
13. Harvey R.W., Smith R.L., George L. Effect of organic contamination upon microbial distributions and heterotrophic uptake in a Cape Cod, Mass., aquifer. Appl. Environ. Microbiol. 48:1984;1197-1202.
14. Kawai M., Yamaguchi N., Nasu M. Rapid enumeration of physiologically active bacteria in purified water used in the pharmaceutical manufacturing process. J. Appl. Microbiol. 86:1999;496-504.
15. Kenner R.A., Ahmed S.I. Correlation between oxygen utilization and electron transport activity in marine phytoplankton. Mar. Biol. 33:1975;129-133.
16. Kim C.W., Koopman B., Bitton G. INT-dehydrogenase activity test for assessing chlorine and hydrogen peroxide inhibition of filamentous pure cultures and activated sludge. Water Res. 28:1994;1117-1121.
17. LeBlanc, D.R., 1984. Sewage plume in a sand and gravel aquifer, Cape Cod, Massachusetts. U.S. Geological Survey Water-Supply, Paper 2218. U.S. Geological Survey, Washington, D.C.
18. Lisle J.T., Pyle B.H., McFeters G.A. The use of multiple indices of physiological activity to access viability in chlorine disinfected Escherichia coli O157:H7. Lett. Appl. Microbiol. 29:1999;42-47.
19. Matson E.A., Buck J.D. Community electron transport of procaroytes in euryoxic estuarine sediment. Mar. Ecol., Prog. Ser. 41:1987;71-78.
20. Metge D.W., Brooks M.H., Smith R.L., Harvey R.W. Effect of treated sewage contamination upon bacterial energy charge, adenine nucleotides, and DNA content in a sandy aquifer on Cape Cod. Appl. Environ. Microbiol. 59:1993;2304-2310.
21. Newell S.Y. Modification of the gelatin-matrix method for enumeration of respiring bacterial cells for use with salt-marsh water samples. Appl. Environ. Microbiol. 47:1984;873-875.
22. Olanczuk-Neyman K.M., Vosjan J.H. Measuring respiratory electron-transport activity in marine phytoplankton. J. Mar. Res. 29:1977;235-244.
23. Owens T.G., King F.D. The measurement of respiratory electron-transport-system activity in marine zooplankton. Mar. Biol. 30:1975;27-36.
24. Packard T.T. The measurement of respiratory electron transport activity in marine phytoplankton. J. Mar. Res. 29:1971;235-244.
25. Packard T.T., Williams P.L.B. Rates of respiratory oxygen consumption and electron transport in surface seawater from the northwest Atlantic Ocean. Oceanol. Acta. 4:1981;351-358.
26. Packard T.T., Garfield P.C., Martinez R. Respiration and respiratory enzyme activity in aerobic and anaerobic cultures of the marine denitrifying bacterium, Pseudomonas perfectomarinus. Deep-Sea Res. 30:1983;227-243.
27. Peñarrieta, C.T., 1998. Assessment of microbial oxygen consumption in a Cape Cod aquifer using a natural gradient tracer test and core incubations. MS thesis, University of Colorado, Boulder, CO.
28. Pickins J.F., Cherry J.A., Grisak G.E., Merritt W.F., Risto B.A. A multilevel device for groundwater sampling and piezometric monitoring. Groundwater. 16:1978;322-327.
29. Proctor L.M., Souza A.C. Method for enumeration of 5-cyano-2,3-ditoyl tetrazolium chloride (CTC)-active cells and cell-specific CTC activity of benthic bacteria in riverine, estuarine and coastal sediments. J. Microbiol. Methods. 43:2001;213-222.
30. Pyle B.H., Broadaway S.C., McFeters G.A. A rapid, direct method for enumerating respiring enterohemorrhagic Escherichia coli O157:H7 in water. Appl. Environ. Microbiol. 61:1995;2614-2619.
31. Ramalho R., Cunha J., Teixeira P., Gibbs P.A. Improved methods for enumeration of heterotrophic bacteria in bottled mineral waters. J. Microbiol. Methods. 44:2001;97-103.
32. Reasoner D.J., Geldreich E.E. A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 49:1985;1-7.
33. Rodriguez G.G., Phipps D., Ishiguro K., Ridgway H.F. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1992;1801-1808.
34. Roslev P., King G.M. Application of a tetrazolium salt with a water-soluble formazan as an indicator of viability in respiring bacteria. Appl. Environ. Microbiol. 59:1993;2891-2896.
35. Schaule G., Flemming H.-C., Ridgeway H.F. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl. Environ. Microbiol. 59:1993;3850-3857.
36. Sherr B.F., del Giorgio P., Sherr E.B. Estimating abundance and single-cell characteristics of respiring bacteria via the redox dye CTC. Aquat. Microb. Ecol. 18:1999;117-131.
37. Smith J.J., McFeters G.A. Effects of substrates and phosphate on INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride) and CTC (5-cyano-2,3-ditolyl tetrazolium chloride) reduction in Escherichia coli. J. Appl. Bacteriol. 80:1996;209-215.
38. Smith R.L., Bohlke J.K., Revesz K.M., Yoshinari T., Hatzinger P.B., Peñarrieta C.T., Repert D.A. In situ assessment of the transport and microbial consumption of oxygen in ground water, Cape Cod, Massachusetts. Morganwalp D.W., Buxton H.T. Subsurface Contamination from Point Sources, vol. 3. U.S. Geological Survey Water-Resources Investigations Report 99-4018C. 1999;317-322.
39. Songster-Alpin M.S., Klotz R.L. A comparison of electron transport system activity in stream and beaver pond sediments. Can. J. Fish. Aquat. Sci. 52:1995;1318-1326.
40. Tabor P.S., Neihof R.A. Improved method for determination of respiring individual microorganisms in natural waters. Appl. Environ. Microbiol. 43:1982;1249-1255.
41. Trevors J.T. Dehydrogenase activity in soil: a comparison between the INT and TTC assay. Soil Biol. Biochem. 16:1984;673-674.
42. Trevors J.T., Mayfield C.I., Inniss W.E. Measurement of electron transport system (ETS) activity in soil. Microb. Ecol. 8:1982;163-168.
43. Ullrich S., Karrasch B., Hoppe H.-G., Jeskulke K., Mehrens M. Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride. Appl. Environ. Microbiol. 62:1996;4587-4593.
44. van Elsas J.D., Kastelein P., de Vries P.M., van Overbeek L.S. Effects of ecological factors on the survival and physiology of Ralstonia solanacearum bv. 2 in irrigation water. Can. J. Microbiol. 47:2001;842-854.
45. Vosjan J.H. Respiratory electron transport system activities in marine environments. Hydrobiol. Bull. 16:1982;61-68.
46. Winding A., Binnerup S.J., Sorensen J. Viability of indigenous soil bacteria assayed by respiratory activity and growth. Appl. Environ. Microbiol. 60:1994;2869-2875.
47. Yu F.P., McFeters G.A. Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes. J. Microbiol. Methods. 20:1994;1-10.
48. Zapico M.M., Vales S., Cherry J.A. A wireline piston core barrel for sampling cohesionless sand and gravel below the water table. Ground Water Monit. Rev. 7:1987;74-82.
49. Zimmermann R., Iturriaga R., Becker-Birck J. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl. Environ. Microbiol. 36:1978;926-935.