Generation of disruption cassettes in vivo using a PCR product and Saccharomyces cerevisiae

Journal of Microbiological Methods

Volumn 52, Issue 1, 2003, Pages 141-145

  Zaragoza, Oscar ^a,b  

^a INSTITUTO DE INVESTIGACIONES BIOMÉDICAS ALBERTO SOLS   (Spain)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Disruption cassette; Homologous recombination; PCR; Saccharomyces cerevisiae

Indexed keywords

OLIGONUCLEOTIDE;

BASE PAIRING; FUNGAL GENE; GENE AMPLIFICATION; GENE CASSETTE; GENE DISRUPTION; GENETIC RECOMBINATION; IN VIVO STUDY; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVIEW; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037208466     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00154-9     Document Type: Article

References (16)

1. Bell W., Klaasen P., Ohnacker M., Boller T., Herweijer M., Schoppink P., van der Zee P., Wiemkem A. Characterization of the 56 kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation. Eur. J. Biochem. 209:1992;951-959.
2. Blázquez M.A., Lagunas R., Gancedo C., Gancedo J.M. Trehalose-6-phosphate, a new regulator of yeast glycolysis that inhibits hexokinases. FEBS Lett. 329:1993;51-54.
3. Duno M., Bendixen C., Krejci L., Thomsen B. Targeted deletions created in yeast vectors by recombinational excision. Nucleic Acids Res. 27:1999;e1.
4. Hill J.E., Myers A.M., Koerner T.J., Tzagoloff A. Yeast/E. coli shuttle vectors with multiple unique restrictions sites. Yeast. 2:1986;163-167.
5. Ito H., Fukada Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:1983;163-168.
6. Maftahi M., Gaillardin C., Nicaud J.M. Sticky-end polymerase chain reaction method for systematic gene disruption in Saccharomyces cerevisiae. Yeast. 12:1996;859-868.
7. Nikawa J., Kawabata M. PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae. Nucleic Acids Res. 26:1998;860-861.
8. Queiros O., Casal M., Moradas-Ferreira P., Leao C. PCR-based method for the construction of gene disruption cassettes in yeast. Biotechniques. 31:2001;264-266.
9. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. 1989;Cold Spring Harbor Laboratories, Cold Spring Harbor, NY.
10. Storici F., Lewis L.K., Resnick M.A. In vivo site-directed mutagenesis using oligonucleotides. Nat. Biotechnol. 19:2001;773-776.
11. van den Berg M.A., Steensma H.Y. Expression cassettes for formaldehyde and fluoroacetate resistance, two dominant markers in Saccharomyces cerevisiae. Yeast. 13:1997;551-559.
12. Wach A., Brachat A., Pohlmann R., Philippsen P. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10:1994;1793-1808.
13. Wach A., Brachat C., Rebischung C., Steiner C., Pokorni K., Heesen S., Philippsen P. PCR-based gene targeting in Saccharomyces cerevisiae. Methods Microbiol. 26:1998;67-81.
14. Wang H., Le Clainche A., Le Dall M.T., Wache Y., Pagot Y., Belin J.M., Gaillardin C., Nicaud J.M. Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane-utilizing yeast Yarrowia lipolytica. Yeast. 14:1998;1373-1386.
15. Willis D., Cook R., Shimer G. Jr., Cottarel G. A system for deletion of Candida glabrata genes and complementation that is amenable to high-throughput. Yeast. 18:2001;S328.
16. Zaragoza O., Blázquez M.A., Gancedo C. Disruption of the Candida albicans TPS1 gene encoding trehalose-6-P synthase impairs formation of hyphae and decreases infectivity. J. Bacteriol. 180:1998;3809-3815.