메뉴 건너뛰기




Volumn 12, Issue 9, 1996, Pages 859-868

Sticky-end polymerase chain reaction method for systematic gene disruption in Saccharomyces cerevisiae

Author keywords

Function analysis; Gene disruption; Saccharomyces cerevisiae; Yeast

Indexed keywords

ARTICLE; CHROMOSOME 14; CONTROLLED STUDY; GENE DISRUPTION; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

EID: 0030037147     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199607)12:9<859::AID-YEA978>3.0.CO;2-Q     Document Type: Article
Times cited : (31)

References (36)
  • 1
    • 0023392267 scopus 로고
    • A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains
    • Alani, E., Cao, L. and Kleckner, N. (1987). A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116, 541-545.
    • (1987) Genetics , vol.116 , pp. 541-545
    • Alani, E.1    Cao, L.2    Kleckner, N.3
  • 2
    • 0027237665 scopus 로고
    • A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
    • Baudin, A., Ozier, K., Denouel, A., Lacroute, F. and Cullin, C. (1993). A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucl. Acids Res. 21, 3329-3330.
    • (1993) Nucl. Acids Res. , vol.21 , pp. 3329-3330
    • Baudin, A.1    Ozier, K.2    Denouel, A.3    Lacroute, F.4    Cullin, C.5
  • 3
    • 0021668558 scopus 로고
    • A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
    • Boeke, J., Lacroute, F. and Fink, G. (1984). A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197, 345-346.
    • (1984) Mol. Gen. Genet. , vol.197 , pp. 345-346
    • Boeke, J.1    Lacroute, F.2    Fink, G.3
  • 5
    • 0028215472 scopus 로고
    • Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae
    • Burns, N., Grimwade, B., Ross-Masdonald, et al. (1994). Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8, 1087-1105.
    • (1994) Genes Dev. , vol.8 , pp. 1087-1105
    • Burns, N.1    Grimwade, B.2    Ross-Masdonald3
  • 6
    • 0020971317 scopus 로고
    • β Galactosidase gene fusions for analyzing expression in Escherichia coli and yeast
    • Casadaban, M., Martinez-Arias, A., Shapira, S. and Chow, J. (1983). β Galactosidase gene fusions for analyzing expression in Escherichia coli and yeast. Methods Enzymol. 100, 293-308.
    • (1983) Methods Enzymol. , vol.100 , pp. 293-308
    • Casadaban, M.1    Martinez-Arias, A.2    Shapira, S.3    Chow, J.4
  • 7
    • 23444431611 scopus 로고
    • Green fluorescent protein as a marker for gene expression
    • Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. and Prasher, D. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
    • (1994) Science , vol.263 , pp. 802-805
    • Chalfie, M.1    Tu, Y.2    Euskirchen, G.3    Ward, W.4    Prasher, D.5
  • 8
    • 8944254284 scopus 로고
    • Improved PCR cloning
    • Costa, G. and Weiner, M. (1995). Improved PCR cloning. Strategies 7, 8.
    • (1995) Strategies , vol.7 , pp. 8
    • Costa, G.1    Weiner, M.2
  • 9
    • 0027953554 scopus 로고
    • Multi-purpose vectors designed for the fast generation of N- or C-terminal epitope-tagged proteins
    • Cullin, C. and Minvielle-Sebastia, L. (1994). Multi-purpose vectors designed for the fast generation of N- or C-terminal epitope-tagged proteins. Yeast 10, 105-112.
    • (1994) Yeast , vol.10 , pp. 105-112
    • Cullin, C.1    Minvielle-Sebastia, L.2
  • 10
    • 0025840490 scopus 로고
    • Gene transfer with subsequent removal of the selection gene from the host genome
    • Dale, E. and Ow, D. (1991). Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 88, 10558-10562.
    • (1991) Proc. Natl. Acad. Sci. USA , vol.88 , pp. 10558-10562
    • Dale, E.1    Ow, D.2
  • 11
    • 0027940725 scopus 로고
    • A genetic screen to isolate genes regulated by the yeast CCAAT-box binding protein Hap2p
    • Dang, V., Valens, M., Bolotin-Fukuhara, M. and Daignan-Fornier, B. (1994). A genetic screen to isolate genes regulated by the yeast CCAAT-box binding protein Hap2p. Yeast 10, 1273-1283.
    • (1994) Yeast , vol.10 , pp. 1273-1283
    • Dang, V.1    Valens, M.2    Bolotin-Fukuhara, M.3    Daignan-Fornier, B.4
  • 12
    • 0028301199 scopus 로고
    • Complete DNA sequence of yeast chromosome XI
    • Dujon, B. et al. (1994). Complete DNA sequence of yeast chromosome XI. Nature 369, 371-378.
    • (1994) Nature , vol.369 , pp. 371-378
    • Dujon, B.1
  • 13
    • 0027988522 scopus 로고
    • Transcript map of two regions from chromosome XI of Saccharomyces cerevisiae for interpretation of systematic sequencing results
    • Fairhead, C. and Dujon, B. (1994). Transcript map of two regions from chromosome XI of Saccharomyces cerevisiae for interpretation of systematic sequencing results. Yeast 10, 1403-1413.
    • (1994) Yeast , vol.10 , pp. 1403-1413
    • Fairhead, C.1    Dujon, B.2
  • 14
    • 0028586779 scopus 로고
    • Complete DNA sequence of yeast chromosome II
    • Feldmann, H. et al. (1994). Complete DNA sequence of yeast chromosome II. EMBO J. 13, 5795-5809.
    • (1994) EMBO J. , vol.13 , pp. 5795-5809
    • Feldmann, H.1
  • 15
    • 0028258384 scopus 로고
    • Approaching the function of new genes by detection of their potential upstream activation sequences in Saccharomyces cerevisiae: Application to chromosome III
    • Fondrat, C. and Kalogeropoulos, A. (1994). Approaching the function of new genes by detection of their potential upstream activation sequences in Saccharomyces cerevisiae: application to chromosome III. Curr. Genet. 25, 396-406.
    • (1994) Curr. Genet. , vol.25 , pp. 396-406
    • Fondrat, C.1    Kalogeropoulos, A.2
  • 16
    • 0002819495 scopus 로고
    • Higth efficiency transformation with lithium acetate
    • Johnston, J. R. (Eds), IRL Press, Oxford, UK
    • Gietz, R. and Woods, R. (1994). Higth efficiency transformation with lithium acetate. In Johnston, J. R. (Eds), Molecular Genetics of Yeast, A Practical Approach. IRL Press, Oxford, UK, pp. 121-134.
    • (1994) Molecular Genetics of Yeast, a Practical Approach , pp. 121-134
    • Gietz, R.1    Woods, R.2
  • 17
    • 0028954118 scopus 로고
    • Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure
    • Gietz, R., Schiestl, R., Willems, A. and Woods, R. (1995). Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11, 355-360.
    • (1995) Yeast , vol.11 , pp. 355-360
    • Gietz, R.1    Schiestl, R.2    Willems, A.3    Woods, R.4
  • 18
    • 0028771517 scopus 로고
    • Genes in search of a function
    • Goffeau, A. (1994). Genes in search of a function. Nature 369, 101-102.
    • (1994) Nature , vol.369 , pp. 101-102
    • Goffeau, A.1
  • 19
    • 3242793191 scopus 로고
    • Direct clone characterisation from plaques and colonies by the polymerase chain reaction
    • Gussow, D. and Clarckson, T. (1989). Direct clone characterisation from plaques and colonies by the polymerase chain reaction. Nucl. Acids Res. 17, 4000.
    • (1989) Nucl. Acids Res. , vol.17 , pp. 4000
    • Gussow, D.1    Clarckson, T.2
  • 20
    • 0025166077 scopus 로고
    • G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae
    • Hadfield, C., Jordan, B., Mount, R., Pretorius, G. and Burak, E. (1990). G418-resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae. Curr. Genet. 18, 303-313.
    • (1990) Curr. Genet. , vol.18 , pp. 303-313
    • Hadfield, C.1    Jordan, B.2    Mount, R.3    Pretorius, G.4    Burak, E.5
  • 21
    • 0028073797 scopus 로고
    • Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII
    • Johnston, M. et al. (1994). Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265, 2077-2082.
    • (1994) Science , vol.265 , pp. 2077-2082
    • Johnston, M.1
  • 22
    • 0029096027 scopus 로고
    • A method for performing precise alterations in yeast genome using a recyclable marker
    • Längle-Rouault, F. and Jacobs, E. (1995). A method for performing precise alterations in yeast genome using a recyclable marker. Nucl. Acids Res. 23, 3079-3081.
    • (1995) Nucl. Acids Res. , vol.23 , pp. 3079-3081
    • Längle-Rouault, F.1    Jacobs, E.2
  • 23
    • 0029042961 scopus 로고
    • Gene disruption with PCR products in Saccharomyces cerevisiae
    • Lorenz, M., Muir, R., Lim, E., McElver, J., Weber, S. and Heitman, J. (1995). Gene disruption with PCR products in Saccharomyces cerevisiae. Gene 158, 113-117.
    • (1995) Gene , vol.158 , pp. 113-117
    • Lorenz, M.1    Muir, R.2    Lim, E.3    McElver, J.4    Weber, S.5    Heitman, J.6
  • 24
    • 0029076323 scopus 로고
    • Sequencing analysis of 15.4 kb of chromosome XIV identifies the RPD3, PAS8 and KRE1 loci and five new open reading frames
    • Maftahi, M., Nicaud, J.-M., Levesque, H. and Gaillardin, C. (1995a). Sequencing analysis of 15.4 kb of chromosome XIV identifies the RPD3, PAS8 and KRE1 loci and five new open reading frames. Yeast 11, 567-572.
    • (1995) Yeast , vol.11 , pp. 567-572
    • Maftahi, M.1    Nicaud, J.-M.2    Levesque, H.3    Gaillardin, C.4
  • 25
    • 0029081693 scopus 로고
    • Sequencing analysis of 24.7 kb of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new ORFs
    • Maftahi, M., Nicaud, J.-M., Levesque, H. and Gaillardin, C. (1995b). Sequencing analysis of 24.7 kb of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new ORFs. Yeast 11, 1077-1085.
    • (1995) Yeast , vol.11 , pp. 1077-1085
    • Maftahi, M.1    Nicaud, J.-M.2    Levesque, H.3    Gaillardin, C.4
  • 26
    • 0029120399 scopus 로고
    • Micro-homology mediated PCR-targeting in Saccharomyces cerevisiae
    • Manivaskam, P., Weber, S. C., McElver, J. and Schiestl, R. H. (1995). Micro-homology mediated PCR-targeting in Saccharomyces cerevisiae. Nucl. Acids Res. 23, 2799-2800.
    • (1995) Nucl. Acids Res. , vol.23 , pp. 2799-2800
    • Manivaskam, P.1    Weber, S.C.2    McElver, J.3    Schiestl, R.H.4
  • 27
    • 0026572003 scopus 로고
    • The complete DNA sequence of yeast chromosome III
    • Oliver, S. G. et al. (1992). The complete DNA sequence of yeast chromosome III. Nature 357, 38-46.
    • (1992) Nature , vol.357 , pp. 38-46
    • Oliver, S.G.1
  • 28
    • 0025979877 scopus 로고
    • Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast
    • Rothstein, R. (1991). Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 194, 281-301.
    • (1991) Methods Enzymol. , vol.194 , pp. 281-301
    • Rothstein, R.1
  • 30
    • 0024799254 scopus 로고
    • High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
    • Schiestl, R. H. and Gietz, R. D. (1989). High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16, 339-346.
    • (1989) Curr. Genet. , vol.16 , pp. 339-346
    • Schiestl, R.H.1    Gietz, R.D.2
  • 31
    • 0025254446 scopus 로고
    • Application of the beta-glucuronidase gene fusion system to Saccharomyces cerevisiae
    • Schmitz, U., Lonsdale, D. and Jefferson, R. (1990). Application of the beta-glucuronidase gene fusion system to Saccharomyces cerevisiae. Curr. Genet. 17, 261-264.
    • (1990) Curr. Genet. , vol.17 , pp. 261-264
    • Schmitz, U.1    Lonsdale, D.2    Jefferson, R.3
  • 32
    • 0030051339 scopus 로고    scopus 로고
    • pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae
    • Schneider, B., Steiner, B., Seufert, W. and Futcher, A. (1996). pMPY-ZAP: a reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae. Yeast 12, 129-134.
    • (1996) Yeast , vol.12 , pp. 129-134
    • Schneider, B.1    Steiner, B.2    Seufert, W.3    Futcher, A.4
  • 33
    • 0028958228 scopus 로고
    • Construction of a marker gene cassette which is repeatedly usable for gene disruption in yeast
    • Toh-e, A. (1995). Construction of a marker gene cassette which is repeatedly usable for gene disruption in yeast. Curr. Genet 27, 293-297.
    • (1995) Curr. Genet , vol.27 , pp. 293-297
    • Toh-e, A.1
  • 34
    • 0028676232 scopus 로고
    • New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae
    • Wach, A., Brachat, A., Pohlmann, R. and Philippsen, P. (1994). New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793-1808.
    • (1994) Yeast , vol.10 , pp. 1793-1808
    • Wach, A.1    Brachat, A.2    Pohlmann, R.3    Philippsen, P.4
  • 35
    • 0029871347 scopus 로고    scopus 로고
    • PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in Saccharomyces cerevisiae
    • Wach, A. (1996). PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in Saccharomyces cerevisiae. Yeast 12, 259-266.
    • (1996) Yeast , vol.12 , pp. 259-266
    • Wach, A.1
  • 36
    • 0028794516 scopus 로고
    • Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C
    • Winston, F., Dollard, C. and Ricupero-Hovasse, S. L. (1995). Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55.
    • (1995) Yeast , vol.11 , pp. 53-55
    • Winston, F.1    Dollard, C.2    Ricupero-Hovasse, S.L.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.