Comparison of pulsed-field gel electrophoresis (PFGE) and two different polymerase chain reactions (PCRs) for species identification of Borrelia burgdorferi sensu lato strains

Wiener Klinische Wochenschrift

Volumn 114, Issue 13-14, 2002, Pages 551-556

  Šimenc, Janez ^a   Ružić Sabljić, Eva ^a   Strle, Franc ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Borrelia burgdorferi; Polymerase chain reaction; Pulsed field gel electrophoresis; Species identification

Indexed keywords

DNA FRAGMENT; GENE PRODUCT; RESTRICTION ENDONUCLEASE; RNA 16S;

ARTICLE; BACTERIUM ISOLATION; BORRELIA AFZELII; BORRELIA BURGDORFERI; BORRELIA GARINII; CONTROLLED STUDY; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; SPECIES DIFFERENTIATION; SPECIES IDENTIFICATION; ANIMAL; CLASSIFICATION; COMPARATIVE STUDY; GENETIC HETEROGENEITY; GENETICS; HUMAN; LYME DISEASE; METHODOLOGY; MICROBIOLOGY; SLOVENIA; SPECIES DIFFERENCE; TICK;

ANIMAL; BORRELIA BURGDORFERI; BORRELIA BURGDORFERI GROUP; COMPARATIVE STUDY; ELECTROPHORESIS, GEL, PULSED-FIELD; GENETIC HETEROGENEITY; HUMAN; LYME DISEASE; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SLOVENIA; SPECIES SPECIFICITY; TICKS; ANIMALS; HUMANS;

EID: 0037205669     PISSN: 00435325     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Phylogenetic analysis of the genus Borrelia: A comparison of north American and European isolates of Borrelia burgdorferi
2. Lyme borreliosis
3. Clinical findings for patients with Lyme borreliosis causing by Borrelia burgdorferi sensu lato with genotypic and phenotypic similarities to strain 25015
4. European reservoir hosts of Borrelia burgdorferi sensu lato
5. Lyme disease-a tick born spirochetosis?
6. Molecular typing of Borrelia burgdorferi sensu lato: Taxonomic, epidemiological and clinical implications
7. Lyme borreliosis in Slovenia
8. Association between different clinical manifestations of Lyme disease and different species of Borrelia bugdorferi sensu lato
9. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis
10. Characterization of Borrelia burgdorferi sensu lato strains isolated from patients with skin manifestations of Lyme borreliosis residing in Slovenia
11. Molecular characterization of Borrelia burgdorferi sensu lato strains by pulsed-field gel electrophoresis
12. Genomic fingerprinting of Borrelia burgdorferi sensu lato by pulsed-field gel electrophoresis
13. Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis
14. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons
15. Molecular typing of Borrelia burgdorferi sensu lato by PCR-restriction fragment length polymorphism analysis
16. Common ancestry of Borrelia burgdorferi sensu lato strains from north America and Europe
17. Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources
18. Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent
19. Linear chromosomes of Lyme disease spirochetes: Genetic diversity and conservation of gene order
20. Genetic diversity of Borrelia burgdorferi in Lyme disease patiens as determined by culture versus direct PCR with clinical specimens
21. The promises and pitfalls of PCR
22. Infection of small mammals with Borrelia burgdorferi sensu lato in Slovenia as determined by polymerase chain reaction (PCR)
23. Genotypic and phenotypic characterization of Borrelia burgdorferi sensu lato strains isolated from human blood
24. Sequence analysis of the ribosomal RNA operon of the Lyme disease spirochete, Borrelia burgdorferi
25. Three species of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii, and B. garinii) identified from cerebrospinal fluid isolates by pulsed-field gel electrophoresis and PCR
26. Simultaneus presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients