A strategy for in vivo screening of subtilisin E reaction specificity in E. coli periplasm

Biotechnology and Bioengineering

Volumn 78, Issue 7, 2002, Pages 761-769

  Sroga, Grazyna E ^a   Dordick, Jonathan S ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Cell based kinetic microtiter assays; E. coil periplasmic expression; Subtilisins

Indexed keywords

BACTERIA; BIOASSAY; CATALYST ACTIVITY; CELL CULTURE; CELLS; CULTIVATION; ENZYMES; ESCHERICHIA COLI; MUTAGENESIS;

PERIPLASMIC FRACTIONS;

BIOTECHNOLOGY;

AMIDASE; ANILIDE; ARABINOSE; ESTER DERIVATIVE; ESTERASE; MUTANT PROTEIN; N ACETYLPHENYLALANYL 4 NITROPHENYL ESTER; N SUCCINYLALANYLALANYLPROLYLPHENYLALANYL 4 NITROANILIDE; N,N DIMETHYLFORMAMIDE; SUBTILISIN; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOPLASM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ESCHERICHIA COLI; IN VIVO STUDY; MICROTITER PLATE ASSAY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN STABILITY; STEADY STATE; TEMPERATURE DEPENDENCE; WESTERN BLOTTING; WILD TYPE;

AMIDOHYDROLASES; ARABINOSE; CATALYSIS; ENZYME STABILITY; ESCHERICHIA COLI; ESTERASES; GENE EXPRESSION; GENE EXPRESSION REGULATION; MODELS, GENETIC; PERIPLASM; SENSITIVITY AND SPECIFICITY; SUBSTRATE SPECIFICITY; SUBTILISINS; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0037199161     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10269     Document Type: Article

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