Protein estimation directly from SDS-PAGE loading buffer for standardization of samples from cell lysates or tissue homogenates before western blot analysis

BioTechniques

Volumn 31, Issue 3, 2001, Pages 478-482

  Chapdelaine, P ^a   Vignola, K ^a   Fortier, M A ^a  

^a Université Laval   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; ARTICLE; CELL LYSATE; COLORIMETRY; IMMUNOBLOTTING; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; STANDARDIZATION;

EID: 0034858142     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01313bm04     Document Type: Article

References (6)

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
2. Quantification of proteins dissolved in an electrophoresis sample buffer
3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
4. Protein estimation with the folin phenol reagent
5. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
6. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids