Biochemical and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans

FEMS Microbiology Letters

Volumn 214, Issue 1, 2002, Pages 127-132

  Inoue, Hiroyuki ^a   Tamura, Takashi ^a   Ehara, Nagisa ^a   Nishito, Akira ^a   Nakayama, Yumi ^a   Maekawa, Makiko ^a   Imada, Katsumi ^b   Tanaka, Hidehiko ^a   Inagaki, Kenji ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Acidithiobacillus thiooxidans; Coenzyme specificity; Decarboxylating dehydrogenase; Enzyme purification; Isocitrate dehydrogenase

Indexed keywords

ACONITATE HYDRATASE; AMINO ACID; BACTERIAL ENZYME; CELL EXTRACT; ISOCITRATE DEHYDROGENASE (NAD); NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; POLYPEPTIDE; SUCCINYL COENZYME A SYNTHETASE;

ACIDITHIOBACILLUS THIOOXIDANS; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BINDING SITE; BIOCHEMISTRY; CONTROLLED STUDY; DECARBOXYLATION; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION; GENE LOCATION; GENE STRUCTURE; MOLECULAR CLONING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN ANALYSIS; REGULATORY MECHANISM;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; GAMMAPROTEOBACTERIA; ISOCITRATE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; NAD; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

ACIDITHIOBACILLUS; ACIDITHIOBACILLUS THIOOXIDANS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 0037183391     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(02)00857-1     Document Type: Article

References (17)

1. Hurley J.H., Thorsness P.E., Ramalingam V., Helmers N.H., Koshland D.E. Jr., Stroud R.M. Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase. Proc. Natl. Acad. Sci. USA. 86:1989;8635-8639.
2. Imada K., Sato M., Tanaka N., Katsube Y., Matsuura Y., Oshima T. Three-dimensional structure of a highly thermostable enzyme, 3-isopropylmalate dehydrogenase of Thermus thermophilus at 2.2 Å resolution. J. Mol. Biol. 222:1991;725-738.
3. Imada K., Inagaki K., Matsunami H., Kawaguchi H., Tanaka H., Tanaka N., Namba K. Structure of 3-isopropylmalate dehydrogenase in complex with 3-isopropylmalate at 2.0 Å resolution: the role of Glu88 in the unique substrate-recognition mechanism. Structure. 6:1998;971-982.
4. Chen R., Greer A., Dean A.M. A highly active decarboxylating dehydrogenase with rationally inverted coenzyme specificity. Proc. Natl. Acad. Sci. USA. 92:1995;11666-11670.
5. + specificity-reversal mutant. Biochemistry. 35:1996;5670-5678.
6. Miyazaki K., Oshima T. Co-enzyme specificity of 3-isopropylmalate dehydrogenase from Thermus thermophilus HB8. Protein Eng. 7:1994;401-403.
7. Hampton M.L., Hanson R.S. Regulation of isocitrate dehydrogenase from Thiobacillus thiooxidans and Pseudomonas fluorescens. Biochem. Biophys. Res. Commun. 36:1969;296-305.
8. Smith A.J., London J., Stanier R.Y. Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. J. Bacteriol. 94:1967;972-983.
9. 2 assimilation via the reductive tricarboxylic acid cycle in an obligatory autotrophic, aerobic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus. Arch. Microbiol. 141:1985;198-203.
10. Kawaguchi H., Inagaki K., Matsunami H., Nakayama Y., Tano T., Tanaka H. Purification and characterization of 3-isopropylmalate dehydrogenase from Thiobacillus thiooxidans. J. Biosci. Bioeng. 90:2000;459-461.
11. Eikmanns B.J., Rittmann D., Sahm H. Cloning, sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme. J. Bacteriol. 177:1995;774-782.
12. Saito M., Miura K. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta. 72:1963;619-629.
13. Miyazaki K. Isocitrate dehydrogenase from Thermus aquaticus YT1: purification of the enzyme and cloning, sequencing, and expression of the gene. Appl. Environ. Microbiol. 62:1996;4627-4631.
14. Cvitkovitch D.G., Gutierrez J.A., Bleiweis A.S. Role of the citrate pathway in glutamate biosynthesis by Streptococcus mutans. J. Bacteriol. 179:1997;650-655.
15. Dean A.M., Golding G.B. Protein engineering reveals ancient adaptive replacements in isocitrate dehydrogenase. Proc. Natl. Acad. Sci. USA. 94:1997;3104-3109.
16. + complexes. Biochemistry. 30:1991;8671-8678.
17. +-dependent isocitrate dehydrogenase. Cloning, nucleotide sequence, and disruption of the ICDH2 gene from Saccharomyces cerevisiae. J. Biol. Chem. 266:1991;22199-22205.